Luo et al. /G09090
Supplementary Information: Amino Acid Sequence and Homology Regions of PID
Protein
Following a large preparation, enough material of the p75 band was obtained for
peptide sequencing, and a total of three peptide sequences were obtained. One of the
peptide sequences, containing the amino acid sequence AQSLSPYTTSAN, was found in
an EST clone (AA928022). Using the cDNA sequence from this EST as a probe, we
isolated a full-length cDNA clone from a HeLa cDNA library that encodes a 668-amino
acid protein. Significantly, the other two peptide sequences (VWDPDNPLTDR and
STLVPQGGPVLC) are also found in the full length PID protein sequence. Interestingly,
a small region (residues 628-663) near the C-terminus contains a similar proline-rich
region found in the N-terminal region of p53 protein, which has been shown to play an
important role in p53-mediated functions. The functional consequence of the structural
similarity between these two proteins remains to be investigated.
Figure legend: Amino acid sequence and homology regions of PID protein. (a) Amino
acid sequence of PID. The three peptide sequences are underlined. (b) Schematic
representation of both MTA-1 homology regions and the proline-rich region on PID
protein. (c) Amino acid alignments between the PID proline-rich region (residues 628663) and the p53 proline-rich region (residues 63-98).
Luo et al. /G09090
a
1
61
121
181
241
301
361
421
481
541
601
661
maanmyrvgd
snarefeees
lekedcffys
pdnpltdrqi
ngydlakams
asivqfyymw
fqkgltcesc
hsrghlsrpe
rpyapinana
pinrnqlsqn
apnpvvfvat
nepivled
yvyfensssn
kqpgvseqqr
lvfdpvqktl
dqflvvarav
tlvpqggpvl
kttdryiqqk
httqsaqwya
aqslspytts
ikaecsirlp
rglggimvkr
kdtralrkal
pylvrrieel
hqlkhrelfl
ladqgeirvg
gtfaraldcs
crdemeewsa
rlkaaeadsk
wgppnmqcrl
anrakllakn
kaaktplkih
ayetmagagv
thlemrraar
nktangnvea
srqfeslpat
ckyqaeipdr
ssirqpslhm
seamlfeeal
lkqvyiptyt
cascwiywkk
rqtfllqttk
plvrlplati
pfsangrpla
rpnlplkvkp
kvvclfrrrd
hirgkcsvtl
lvegesdnrn
saaaasrdit
ekygkdfndi
kpnpnqiisv
ygglktptql
ltrlarrmcr
vkdlvaqapl
sgirsssqpa
tliavrppvp
issslnslad
lnetdilsqy
qqkmemkvwd
lfhamdtlqr
rqdflpwksl
gskpgmngag
egatrgttep
dllqprraar
kpktprgtkt
akrqklnpad
lpapshpast
b
1
668
MTA-1 Homology Region
Proline-Rich Region
c
PID
628
AARRPNLPLKVKPTLIAVRPPVPLPAPSHPASTNEP
663
p53
63
APRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVP
98