International meeting of the DFG priority program 1170 and the GBM study group “Protein Engineering and Design” Thursday, 17.09.09 14.00-14.15 Welcome addresses by N. Raffler (DFG) and R. Sterner Chair: R. Sterner 14.15-15.00 D. Hilvert: Coupling computation and evolution for enzyme design 15.00-15.45 R. Armstrong: Evolution of catalytic diversity in the VOC superfamily 15.45-16.15 Coffee 16.15-17.00 M. Danson: Catalytic promiscuity in the hyperthermophilic Archaea: from metabolic efficiency to engineering stereoselectivity 17.00-17.30 J. Reinstein: Tailoring molecular chaperones - in vivo and in vitro selection approaches for improved folding assistance 17.30-18.00 J. Pleiss: Molecular dynamics simulation of protein hydration 18.00-19.30 Dinner in the restaurant “Kneitinger” 19.30-20.15 A. Plückthun: Designing, evolving and exploiting protein stability Friday, 18.09.09 Chair: H. Kolmar 8.30-9.00 W. Wende: Generation of meganucleases of pre-defined specificity by rational and combinatorial strategies 9.00-9.30 A. Jeltsch: Directed evolution of DNA methyltransferases 9.30-10.00 S. Brakmann: Directed evolution of polymerases with reduced fidelity 10.00-10.30 Coffee 10.30-11.15 L. Loeb: Involvement of mutator DNA polymerases in carcinogenesis 11.15-11.45 A. Marx: Translesion synthesis across non-instructive abasic DNA sites by DNA polymerase 11.45-12.45 Lunch 12.45-13.30 S. Lutz: Making the lock fit the key - engineering kinases to phosphorylate nucleoside analogs for antiviral and cancer therapy 13.30-14.15 P. Bryan: A minimal sequence code for switching protein structure and function 14:15-14:30 J. Podtetenieff: A new artificial metalloenzyme: Creation of a copper binding site in a thermostable protein 14:30-15:00 Coffee 15.00-15.30 K. Müller: Evolutionary optimization of enzyme stability and of enzyme fragment folding for complementation assays 1 15.30-16.00 F. Schmid: Dimer formation of a stabilized G1 variant: A structural and energetic analysis Chair: F. Schmid 16.00-16.30 U. Bornscheuer: (Focused) directed evolution to understand relationships in α/β hydrolase-fold enzymes 16.30-17.00 H. Kolmar: Novel display strategies for high throughput screening of enzyme libraries 17.00-17.30 M. Stubbs: Directed evolution of the S1 subsite specificity of trypsin 17.30-19.00 Dinner in the restaurant “Kneitinger” 19.00-19.45 J. Gerlt: Predicting and discovering new reactions in the Enolase and RuBisCO superfamilies 19.45-20.30 F. Raushel: Annotating orphan enzymes Saturday, 19.09.09 Chair: A. Jeltsch 8.30-9.15 E. Westhof: Elementary modules of RNA architecture 9.15-10.00 J. Burke: GlmS ribozyme: Folding and catalysis 10.00-10.30 R. Hartmann: Insights into RNase P RNA structure and function by a "retro-evolution" approach 10.30-11.00 Coffee 11.00-11.30 M. Mörl: In vitro evolution of nucleotidyltransferases 11.30-12.00 J. Müller: Structure and function of new oligomeric protein species of human PAPS synthetases 12.00-12.30 A. Samland: Changing the substrate scope of transaldolase B towards nonphosphorylated compounds 12.30-13.30 Lunch 13.30-14.30 Meeting of SPP 1170 and Protein Engineering & Design study group members 14:30-16:30 Guided tour through the Old City of Regensburg Chair: A. Marx 16.30-17.00 B. Höcker: Evolutionary relationships beyond fold boundaries 17.00-17.30 M. Wilmanns: Catalytic promiscuity in an ancestral isomerase from Mycobacterium tuberculosis by active site metamorphosis 17.30-18.00 M. Bocola: Analysis and redesign of ()8-barrel stability and activity 18.00-18.30 R. Sterner: Activation of a thermostable enzyme by removal of Mg2+-inhibition and acceleration of product release 18.30 Dinner in the restaurant “Fürstliches Brauhaus” Sunday, 20.09.09 Departure after breakfast 2