Enzyme Design - Substrate and Ligand Recognition,
Rational and Combinatorial Strategies
International meeting of the DFG priority program 1170
and the GBM study group “Protein Engineering & Design”
Evangelische Akademie Bad Herrenalb (September 29 – October 3, 2007)
Saturday, 29.09.07
17.30
Welcome address by N. Raffler (DFG), R. Sterner (SPP 1170) and A. Skerra
(GBM study group)
18.00
Dinner
Chair: A. Pingoud
19.30
L. Loeb (Seattle, USA): Random mutations in human cancers: origin and
consequences
20.15
F. Pâques: (Romainville, F): Meganucleases: when protein engineering meets
genome engineering
Sunday, 30.09.07
Chair: H. Kolmar
8.30-9.00
U. Schwaneberg (Bremen): Directed evolution of P450 BM-3 for alternative
cofactor systems
9.00-9.30
M. Reetz (Mühlheim): Methodology Development in Directed Evolution: Iterative
CASTing
9.30-10.00
U. Bornscheuer (Greifswald): Directed evolution to introduce promiscuous
activity into an esterase
10.00-10.30
Coffee Break
10.30-11.00
M. Pohl (Jülich): How thiamine-dependent enzymes control stereoselectivity
11.00-11.30
G. Sprenger (Stuttgart)/W.-D. Fessner (Darmstadt): Directed evolution of
transaldolase B from E. coli towards novel substrate specificities
11.30-12.00
H. Kolmar (Darmstadt)/K.-E. Jaeger (Düsseldorf/Jülich): Enantioselectivity
screen by two-colour-FACS
12.00-14.00
Lunch Break
Chair: R. Sterner
14.00-14.30
A. Skerra (München): The role of hypervariable loops for antigen recognition:
insights from structural analyses of engineered lipocalins
14:30-14:45
U. Binder (München): Strategies for oligomerisation and cell-surface display of
engineered lipocalins
14.45-15.00
B. Höcker (Tübingen): Evolution and design of ()8-barrels from flavodoxin-like
proteins
15.00-15.30
M. Wilmanns (Hamburg): Structure, function and selective inhibition of the
bisubstrate enzyme PriA
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15.30-16.00
M. Bocola (Regensburg): Analysis and redesign of ()8-barrel stability and
activity
16.00-16.30
Coffee Break
16.30-16.45
W. Gronwald (Regensburg): Verification and falsification of protein-protein
interactions by use of docking techniques
16.45-17.00
R. Merkl (Regensburg): Computational analysis of protein-protein interfaces
using multiple sequence alignments
17.00-17.30
J. Pleiss (Stuttgart): Structural and dynamic properties of conserved water
molecules in class A -lactamases
17.30-18.00
K. Müller (Freiburg): Exploiting trait linkage and evaluating non-immunogenicity
with -lactamase
18.00
Dinner
Chair: F.X. Schmid
19.30
D. Pompon (Gif-Sur-Yvette, F): Statistical approaches of function space analysis
in combinatorial libraries of mammalian cytochrome P450s
20.15
R. Glockshuber (Zürich, CH): Assembly of supra-molecular protein complexes:
type 1 pili from pathogenic E. coli strains as a model system
Monday, 01.10.07
8.30-9.15
E. Westhof (Strassburg, F): RNA evolution and the search for new ribozymes
and riboswitches
9.15-10.00
J. Burke (Burlington, USA): Constructing the active site of a small ribozyme
10.00-10.30
Coffee Break
10.30-11.00
F.X. Schmid (Bayreuth): Stabilisation of the phage gene-3-protein by in vitro
selection: molecular basis of the observed stabilizing effects
11.00-11.30
C. Lange (Halle): Design of highly stable artificial proteins by a tetrapeptide
fragment-based method
11.30-11.45
D. Hoffmann (Duisburg-Essen): Evolutionary optimization of conformationally
stable peptides
11.45-12.15
W. Haehnel (Freiburg): From combinatorial synthesis to the design of synthetic
proteins from first principle: challenging our understanding of protein folding
12.15-12.30
H. Decker (Mainz): Blue blood: New insights into evolution and catalysis
12.30-14.30
Lunch break
14.30-15.30
Meeting of SPP1170 and Protein Engineering & Design study group members
Chair: U. Schwaneberg
15.30-16.00
W. Hoehne (Berlin): Selection and optimization of peptide chitinase inhibitors
from a phage display library
16.00-16.30:
C. Buchholz (Langen): Retroviral particles as novel platform for protein display
and directed evolution
16.30-17.00
Coffee break
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17.00-17.15
R. Süßmuth (Berlin): Chemoenzymatic approach to the synthesis of new
cyclopeptides by enniatin synthetase
17.15-17.30
M. Schlapschy (München): A system for concomitant overexpression of four
periplasmic folding catalysts to improve secretory protein production in E. coli
17:30-17.45
M. Rubini (München): New tools for expanding the genetic code
17.45-18.15
K. Blank (Strassburg, F): Analysis of the evolution of enzyme conformations
during directed in vitro evolution
18.15
Dinner
Chair: A. Skerra
19.30
L. Riechmann (Cambridge, UK): Evolution of novel protein domains
20.15
A. Plückthun (Zürich, CH): The taming of the shrew: Evolving GPCRs
Tuesday, 02.10.07
8.30-9.15
A. Bommarius (Atlanta, USA): Beyond combinatorial protein engineering:
examples of biocatalyst improvement with data-driven protein engineering
9.15-10.00
D. Hilvert (Zürich, CH): Molecular diversity and catalysis
10.00-10.30
Coffee Break
10.30-11.00
R. Sterner, C. Seidel (Regensburg/Düsseldorf): Monomerisation of a homodimeric enzyme by rational design
11.00-11.30
H. Betat (Leipzig): Reanimation of a CCA adding enzyme
11.30-12.00
A. Jeltsch (Bremen): Directed evolution for changing the target specificity of
DNA methyltransferases and to map protein/protein interaction sites
12.00-14.30
Lunch Break
Chair: A. Pingoud
14.30-15.00
A. Marx (Konstanz): Modulation of DNA polymerase function by combinatorial
enzyme design
15.00-15.30
W. Wende/A. Pingoud (Giessen): Generation of homing endonucleases of predefined specificity by directed evolution
15.30-16.00
S. Brakmann (Dortmund): Generation of error-prone polymerase variants using
directed evolution
16.00-16.30
Coffee Break
16.30-17.00
F. Bordusa (Halle): Directed evolution of the S1 subsite specificity of trypsin
17.00-17.15
M. Cirefice (Penzberg): Engineering of a protease for diagnostic use
17.15-17.30
J. Mueller (Duisburg/Essen): A recently evolved mitochondrial targeting peptide
17.30-18.00
C. Zeilinger (Hannover): The challenge to handle membrane proteins – what we
learn from the physiological function of ion channels
18.00
Dinner
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Chair: R. Sterner
19.30
F. Raushel (College Station, USA): Annotating proteins of unknown function
20.15
J. Gerlt (Urbana-Champaign, USA): Discovering and predicting new functions in
the enolase superfamily
Wednesday, 03.10.07
Breakfast and departure
We thank the SeSaM Profit Center for financial support.
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