2014 MRC성과발표회
Abstract Information
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< Abstract Sample >
Systemic proteomic analysis for mouse microglia, astrocytes and secretomes
Youngsoo Kim
Departments of Biomedical Sciences and Biomedical Engineering, Seoul National University College
of Medicine, 103 Daehak-ro, Seoul 110-799 Korea
Neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease are related
with neuro-inflammation. Several study of the diseases had revealed that microglia plays a role in the
central nervous system (CNS) by either protective or damage to neuronal cells depending on their
state of activation. We are focusing on the detrimental activation of microglia which releases
neurotoxic molecules including nitric oxide (NO), or a series of pro-inflammatory cytokines mediated
neuronal cell loss in the brain region. Quantitative proteomic approach with stable isotope dimethyl
labeling was applied to identify the differentially expressed proteins (DEPs) on activated BV-2
microglia cell line with lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Thousands of proteins
were identified and quantified for the intracellular and the secreted proteins of mouse BV-2 cell line
by high-resolution LC-MS/MS.
Furthermore, the in-depth post-translational modification (PTM) proteome dataset for mouse
astrocytes were utilized to analyze of pathway associated the neurodegenerative diseases. Our data
represents a valuable resource for systematic functional analysis of potential new drug targets and
biomarkers for neurodegenerative diseases.
References
[1] Dohyun Han, Jonghwa Jin, Jiyoung Yu, Kyunggon Kim and Youngsoo Kim (2014) Integrated
approach using multistep enzyme digestion, TiO 2 enrichment, and database search for in-depth
phosphoproteomic profiling, Proteomics, DOI: 10.1002/pmic.201400102
[2] Dohyun Han, Jonghwa Jin, Jongmin Woo, Hophil Min, and Youngsoo Kim (2014) Proteomic
analysis of mouse astrocytes and their secretome by a combination of FASP and StageTipbased, high pH, reversed-phase fractionation, Proteomics, 14(13-14):1604-1609
[3] Dohyun Han, Sungyoon Moon, Yikwon Kim, Hophil Min, Youngsoo Kim (2014) Characterization of
the membrane proteome and N-glycoproteome in BV-2 mouse microglia by liquid
chromatography-tandem mass spectrometry, BMC Genomics, 15:95
Keywords: Proteomics, PTM, Neurodegenerative diseases, microglia, astrocyte
2014
MRC 성과발표회
Name : 김영수
Affiliation : 서울대학교 의과대학 의공학교실
Position : 교수
E-mail : biolab@snu.ac.kr
Field of Expertise :
Proteomics, biomarker development, molecular diagnostics
Education :
Year
Institute
Position
1978-1982
서울대학교 공과대학 화학생물공학부
공학사
1982-1984
KAIST 생명과학과
이학석사
1987-1992
University of Texas at Austin, Dept. of Chem/Biochem
PhD
Professional Experience : (less than 5 experiences)
Year
1992-1994
Institute
Yale University
Position
Post-doc
1994-1999
영남대학교
부교수
1999-2001
University of Washington, School of Medicine
staff
2002-현재
서울대학교 의과대학
교수
Selected Publications : (less than 5 papers)
[1] Sungyoon Moon, Dohyun Han, Yikwon Kim, Jonghwa Jin, Won-Kyung Ho, and Youngsoo Kim
(2014) Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic
beta-cells by affinity purification-mass spectrometry, Scientific Reports 4, Article number 4376
doi:10.1038/srep04376
[2] Dohyun Han, Sungyoon Moon, Yikwon Kim, Hophil Min, Youngsoo Kim (2014) Characterization of
the membrane proteome and N-glycoproteome in BV-2 mouse microglia by liquid chromatographytandem mass spectrometry, BMC Genomics, 15:95
[3] Jacob J. Kennedy, Susan E. Abbatiello, Kyunggon Kim, Ping Yan, Jeffrey R. Whiteaker, Chenwei
Lin, Jun Seok Kim, Yuzheng Zhang, Xianlong Wang, Richard G. Ivey1, Lei Zhao, Hophil Min, Youngju
Lee, Myeong-Hee Yu, Eun Gyeong Yang, Cheolju Lee4, Pei Wang, Henry Rodriguez, Youngsoo Kim*,
Steven A. Carr, Amanda G. Paulovich (2014) Demonstrating the feasibility of large-scale development
of standardized assays to quantify human proteins, Nature Methods, 11(2):149–155 (*Seoul site
correspondence)
[4] Yu Jin Hwang, Dohyun Han, Sun-Joon Min, Neil W. Kowall, Junghee Lee, Youngsoo Kim* and
Hoon Ryu (2014) ESET methylates UBF at K232/254 and regulates nucleolar heterochromatin
plasticity and rDNA transcription, Nucleic Acids Research, 42(3):1628-43
(*co-correspondence)