MIT Biology Department
7.012: Introductory Biology - Fall 2004
Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. Claudette Gardel
7.012 Solutions to:
Applications of recombinant DNA technology
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ddG ddA ddT ddC
+
a)
5' C T G A A T T A C G T 3'
b) N o, T A 6's D N A sequence for gene K is different than that found on the note.
c) S ee above
d) T A 1 and T A 5. T heir D N A samples at the gene L locus do not match the sample found
on the bag.
e) T he strategy i s as fol l ows:
Y ou design the following two primers to PC R amplify position Z .
f)
i)
primer 1
primer 2
5'GTGCA3'
3'GCTGC5'
1: T A 2
2: T A 5
3: T A 1
4: T A 3 or T A 6
5: T A 4
6: T A 3 or T A 6
i i) C hi a must have commi tted thi s cri me. O nl y T A 6 and T A 3 match the sampl e
tak en f rom the note. T A 6 i s no l onger a suspect due to part a.