DNA Sequencing Request Form - DNA Sequencing and Analysis

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Central Michigan University - DNA Sequencing Request Form
Department of Biological Sciences – Room 208 Brooks Hall
Mount Pleasant, MI 48859 (989) 774-2577 FAX-(989) 774-3462
Contact name: Janet Miller [email protected]
*****Request forms may be submitted to [email protected]*******
Date:
Name:
Email address:
Department:
Account#:
Phone:
Please provide your combined template, water and primer in a clearly marked tube
at the following amounts and a final volume of 7.5 ul (6.22 ul if I am adding primer).
Primers available without additional fees: T7, SP6, M13F and M13R. Please specify if
the facility will be supplying primers.
Template
PCR Product:
100-200 bp
200-500 bp
500-1000 bp
1000-2000 bp
>2000 bp
Amount
10 ng
30 ng
60 ng
120 ng
260 ng
Plasmid
2,000-15,000 bp
200 ng
Primer:
3.2 pmol (ie: 1.28 ul of 2.5pmol/ul)
From our experience, it is CRITICALLY IMPORTANT that the DNA template be clean
and free of contaminants. Please submit samples resuspended in STERILE ddH2O or Tris
and NOT TE.
Pricing Schedule:
On-campus users:
Off-campus users:
$5.50* per reaction
$7.50* per reaction
- Please specify size of Template (ie: 1000 bp): _________________________________
*****Prices subject to change
Name:
Sample Name
Primer (if I
Sample Name
am adding)
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Additional Comments:
Total Cost:
*****Prices subject to change
Primer (if I
am adding)
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