Central Michigan University - DNA Sequencing Request Form Department of Biological Sciences – Room 208 Brooks Hall Mount Pleasant, MI 48859 (989) 774-2577 FAX-(989) 774-3462 Contact name: Janet Miller mille16j@cmich.edu *****Request forms may be submitted to mille16j@cmich.edu******* Date: Name: Email address: Department: Account#: Phone: Please provide your combined template, water and primer in a clearly marked tube at the following amounts and a final volume of 7.5 ul (6.22 ul if I am adding primer). Primers available without additional fees: T7, SP6, M13F and M13R. Please specify if the facility will be supplying primers. Template PCR Product: 100-200 bp 200-500 bp 500-1000 bp 1000-2000 bp >2000 bp Amount 10 ng 30 ng 60 ng 120 ng 260 ng Plasmid 2,000-15,000 bp 200 ng Primer: 3.2 pmol (ie: 1.28 ul of 2.5pmol/ul) From our experience, it is CRITICALLY IMPORTANT that the DNA template be clean and free of contaminants. Please submit samples resuspended in STERILE ddH2O or Tris and NOT TE. Pricing Schedule: On-campus users: Off-campus users: $5.50* per reaction $7.50* per reaction - Please specify size of Template (ie: 1000 bp): _________________________________ *****Prices subject to change Name: Sample Name Primer (if I Sample Name am adding) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Additional Comments: Total Cost: *****Prices subject to change Primer (if I am adding) 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64