Technical Laboratory Bulletin Topic: Her-2/neu gene amplification, FISH Test Name: Test Code: Method: Specimen: Test Schedule: Date: 1/1/05 Her-2/neu gene amplification, FISH FH2N Fluorescent in-situ Hybridization, FISH 2 H&E stained slides, 3 unstained slides Every Monday Indications: Used as a prognostic factor in stage II, node-positive breast cancer patients. An aid to predict disease-free and overall survival of the above patients when treated with cyclophosphamide, doxorubicin and 5-fluorouracil. Breast cancer patients with a 2+ IHC Her-2/neu stain who are candidates for Herceptin treatment. Result Reporting: Non-Amplified or Amplified with the ratio of control to Her-2/neu genes present. References: http://www.vysis.com/BreastCancer_27.asp Additional Information: Contact Dr. Gene R. Putz, Ph.D., Laboratory Director, NorDx, (207) 885-7809 or Email at putzg@mmc.org.