Lesson Plan Summary Sheet
Teacher:
Howerton
Subject:
Biotech 2
Topic:
Unit B1: An Overview of Gene Expression
Date
Essential Question(s)
Activities
How does the structure of
DNA relate to its function?
What happens to DNA during
replication
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How does the structure of
RNA relate to its function?
What happens in the cell
during transcription?
What happens in the cell
during translation?
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Web-Walk
View RNA and Transcription portion of
the Powerpoint*
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Web-Walk
View Translation and Proteins portion of
Powerpoint*
9/22
How do mutations affect gene
expression?
9/23
How can DNA be inserted
into a cell?
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Web-Walk
View Mutation portion of Powerpoint*
Complete the Dry Lab activity
Discuss transformation and the lac operon
Pour plates for Transformation Lab
Streak Starter plate for Transformation Lab
9/17
9/18
9/21
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Web-Walk
View DNA and Replication portion of the
Powerpoint
9/24
How can DNA be inserted
into a cell?
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Complete Bacterial Transformation lab
with pGLO
9/25
How can DNA be inserted
into a cell?
9/28
How can proteins be taken out
of a cell?
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Look at Bacterial Results
Write up lab and answer questions
Start overnight for GFP purification
Complete the GFP Purification Lab
9/29
What occurs during gene
expression?
What occurs during gene
expression?
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Review and Wrap-up
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Unit C Test : Gene Expression
Work on Research Project
Turn in Learning Log & Vocab
9/30
Vocabulary
Learning Log
Deoxyribose
Phosphate
Antiparallel
Helicase
Double helix
Ozaki fragments
Free nucleotides Polymerase
Nitrogen bases (A,T,G,C)
Complementary base pairing
Ribose
Uracil
Transcriptase
mRNA
tRNA
rRNA
C1: Explain how DNA can carry the
genetic information for all humans
yet remain essentially the same for
everyone.
Ribosome
Codon
Anticodon
Traits
Peptide bond
Protein
Polypeptide
Amino acid
Insertions
Deletions
Substitution
Frameshift
Point mutation
Aseptic technique
Plating samples
Streaking for isolation
Antibiotic screening
none
C3: Create a schematic of the events
of translation and the different types
of protein structure.
Transformation effeciency
Purification
Chromatography
Elution
No new vocabulary
No new vocabulary
C2: Create a schematic of the events
of transcription and RNA processing
C4: Describe the link between DNA,
mutations, and traits
C5: Create a flow chart for a
bacterial transformation
C6: Write an explanation for how
this inserted DNA is going to change
the phenotype of this bacterial cell.
C7: Create a flow chart for the
purification of proteins from a cell.
C8: Explain how chromatography
can be used to purify proteins from
the cell.
C9: What is your plan for studying
this material and did you achieve it?
C10:Reflect on your effort and what
you have learned during this unit.
Gene Expression Web-Walk
A. DNA and replication
1. Building a DNA molecule http://learn.genetics.utah.edu/content/basics/
2. From NOVA http://www.pbs.org/wgbh/nova/genome/facts.html
3.DNA replication (from the following site, address the terms below)
http://www.wiley.com/college/boyer/0470003790/animations/replication/replication.htm
http://www.johnkyrk.com/DNAreplication.html
http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf *
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/dna-rna2.swf *
http://bcs.whfreeman.com/thelifewire/content/chp11/1102002.html
*if swf files do not play, cut and paste the address into the URL field of Windows Media Player
DNA Structure
nucleotide subunits
purines
pyramidines
phosphodiestere bonds
hydrogen bonds
How many bonds between A and T? between C and G
What do the numbers 3” and 5’ mean?
Replication
Topoisomerase
SSB proteins
Helicase
Replication bubble
Replication fork
RNA primer/primase
DNA polymerase III
Leading Strand
Lagging Strand
Ozaki fragments
DNA Polymerase I
Ligase
In which direction are new strands of DNA made?
B. Transcription and translation
1. http://learn.genetics.utah.edu/content/molecules/transcribe/
2. From the following site, address the terms below
http://bcs.whfreeman.com/thelifewire/content/chp12/1202001.html
http://www.johnkyrk.com/DNAtranscription.html
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf
http://www.biostudio.com/d_%20Transcription.htm
Transcription
What is it?
Where does it occur?
3 steps
Promoter
TATA box
RNA polymerase II
Elongation
Which direction?
Uracil
Termination
RNA Processing
At 5’ end?
At 3’ end?
Introns
Exons
Splicesome
RNA transcript
3. Translation sites:
http://vcell.ndsu.edu/animations/translation/index.htm
http://www.biostudio.com/demo_freeman_protein_synthesis.htm
http://www.brookscole.com/chemistry_d/templates/student_resources/shared_resources/animations/protein_synthesis/protein_synthesis.htm
l
4. Codon Chart http://www.biologycorner.com/APbiology/DNA/14-1_function_genes.html#.UwIUxm8xrSg
Translation
What is it?
Where does it occur?
3 steps?
Initiator
tRNA
codon
E
P
A
3 steps to elongation?
Peptide bond
Polypeptide
Release factor
C. Mutations
http://highered.mcgraw-hill.com/sites/0072556781/student_view0/chapter11/animation_quiz_4.html
http://www.dnai.org/text/mediashowcase/index2.html?id=605
http://plato.acadiau.ca/courses/biol/Microbiology/Mutation.htm
What are the different types of mutations?
What are the different results of mutations?