READING FOR LECTURES:
Lecture 1:
1. Current state and prospects of macromolecular crystallography.
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):1-11
Dauter Z
2. Turning protein crystallization from an art into a science.
Curr Opin Struct Biol. 2004 Oct;14(5):577-83.
Chayen N
Lecture 2:
1. Validation of protein crystal structures.
Acta Crystallogr D Biol Crystallogr. 2000 Mar;56 ( Pt 3):249-65. Review.
Kleywegt GJ.
2. Not your average density.
Structure. 1997 Dec 15;5(12):1557-69. Review.
Kleywegt GJ, Read RJ.
Lecture 3:
Case study papers and coot tutorial
REFERENCES FOR SHORT PRESENTATION:
Crystallization:
1. Methods for separating nucleation and growth in
Prog Biophys Mol Biol. 2005 Jul;88(3):329-37. Review.
Chayen N
2. Rational protein crystallization
Structure. 2004 Apr;12(4):529-35.
protein crystallisation.
by mutational surface engineering.
Derewenda ZS
3. Crystallization
of foot-and-mouth disease virus 3C protease: surface mutagenesis and a
novel crystal-optimization strategy.
Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):646-50. Epub 2005 Apr 20.
Birtley JR, Curry S.
4. Seeds to crystals
J Struct Biol. 2003 Apr;142(1):66-76. Review
Bergfors T.
Heavy atom derivative
1. Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene
terephthalate bottles using auto-induction medium.
Protein Expr Purif. 2005 Apr;40(2):256-67.
Sreenath hk et al.
2. The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure
determination.
Nat Biotechnol. 2004 Oct;22(10):1297-301
Xie J, Wang L, Wu N, Brock A, Spraggon G, Schultz PG.
3. Novel approach to phasing proteins: derivatization by short cryo-soaking with halides.
Acta Crystallogr D Biol Crystallogr. 2000 Feb;56 ( Pt 2):232-7.
Dauter Z, Dauter M, Rajashankar KR.
4. Expanding the Genetic Code.
Annu Rev Biophys Biomol Struct. 2006 Jan 13
Wang L, Xie J, Schultz PG
Phasing methods:
1. The importance of alignment accuracy for molecular replacement.
Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1229-36.
Schwarzenbacher R, Godzik A, Grzechnik SK, Jaroszewski L.
2. Jolly SAD.
Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):494-506. Epub 2002 Feb 21.
Dauter Z, Dauter M, Dodson E.
3. Selenomethionyl proteins produced for analysis by multiwavelength anomalous
diffraction (MAD): a vehicle for direct determination of three-dimensional structure.
EMBO J. 1990 May;9(5):1665-72.
Hendrickson WA, Horton JR, LeMaster DM.
4. Advances in multiple wavelength anomalous diffraction crystallography.
Curr Opin Chem Biol. 2000 Oct;4(5):495-9. Review.
Ealick SE
Solvent modification and NCS averaging:
1. Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its
receptor Gb3.
Biochemistry. 1998 Feb 17;37(7):1777-88.
Ling H, Pannu NS, Boodhoo A, Armstrong GD, Clark CG, Brunton JL, Read RJ.
2. The crystal structure of the bacterial chaperonin GroEL at 2.8 A.
Nature. 1994 Oct 13;371(6498):578-86.
Braig K, Otwinowski Z, Hegde R, Boisvert DC, Joachimiak A, Horwich AL, Sigler PB.
Validation:
1. Checking your imagination: applications of the free R value.
Structure. 1996 Aug 15;4(8):897-904. Review.
Kleywegt GJ, Brunger AT.
Application (case study):
1. Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte
enhancer factor-2.
Nature. 2003 Apr 17;422(6933):730-4.
Han A, Pan F, Stroud JC, Youn HD, Liu JO, Chen L.
2. Structure of a fibronectin type III domain from tenascin phased by MAD analysis of
the selenomethionyl protein.
Science. 1992 Nov 6;258(5084):987-91.
Leahy DJ, Hendrickson WA, Aukhil I, Erickson HP.