Representative freshwater reserves: better late than never Jon Nevill*, Ngaire Phillips** * Only One Planet ** NIWA Australia ABSTRACT Representative reserves (protected areas) are an accepted component of terrestrial and marine biodiversity conservation programs. Additionally, representative reserves have important values in protecting ecosystems of special importance, and in providing ecologically-based benchmarks useful in assessing the sustainability of management programs. However, in spite of international and national commitments, Australian State governments have been slow to establish systems of representative reserves in freshwater environments. A central recommendation involves the establishment of agreed national methods for the classification of freshwater ecosystems into (‘representative’) categories which can be incorporated into a comprehensive national inventory. A second key recommendation involves the development of a national approach to identifying gaps in the existing reserve system relating specifically to freshwater ecosystems. INTRODUCTION Representative reserves The term “protected areas” is preferred to “reserves” in general use; however the shorter “reserves” is used throughout this paper for the sake of brevity. “Reserves” has a variety of restrictive and legal connotations which can confuse lay readership. The term here is used to mean a defined area subject to a particular management regime designed to protect the area’s ecosystems from threats. The longer phrase “protected areas” thus is more self-explanatory, less threatening, and is in line with international IUCN usage. In arguing for the establishment of systems of representative freshwater reserves, we are not suggesting that these reserves are all that is necessary to protect freshwater biodiversity: far from it. As discussed below, the protection of biodiversity rests on two central platforms: the establishment of special areas to protect representative examples of ecosystems, AND programs to protect biodiversity values across the general landscape. This second platform includes both programs to protect natural values within managed ecosystems (the installation of fishways on weirs and dams, for example), as well as special purpose reserves to protect wilderness, recreational, scenic, cultural or commercial values. Representative reserves are areas selected to protect representative examples of natural ecosystems, features or phenomena. They are established for the: protection of biodiversity through preservation of representative examples of ecosystems, and protection of the species and genotypes which depend on that ecosystem; protection of threatened ecological communities and species; preservation of unique, rare or outstanding botanical, zoological or geological phenomena; 1 the establishment of ecological benchmarks by which to evaluate long-term changes in ecosystems subject to intensive modification (eg: through water abstraction, or the harvesting of plants or animals); protection of important landscape, wilderness, recreational, scientific, cultural and educational values and uses associated with the natural environment, to the extent that such activities are compatible with other objectives. The development of comprehensive, adequate and representative reserves in terrestrial environments is relatively well established. This terminology1 (and the process behind it) is currently being applied to the marine environment, driven primarily by concerns relating to the protection of biodiversity, and encompassing related secondary objectives (see discussion below). Although most Australian states have made policy-level commitments to establish systems of representative freshwater reserves, these commitments, for the most part, have not been implemented (see discussion below). With growing emphasis on biodiversity conservation and sustainable management, the concept of representative freshwater reserves is becoming increasingly relevant. Moreover, the continuing degradation of most of the nation's freshwater ecosystems makes the concept both more relevant and more urgent. Scope The scope of the discussion paper includes all inland wetlands using the Ramsar Convention definition of “wetland”. This definition2 (in brief) encompasses both fresh and saline, flowing and still, and surface and subterranean ecosystems. In other words, the discussion paper covers rivers, lakes, wetlands (using the more limited definition of wetlands current in Australia), aquifers and karst systems, and estuaries whose ecosystems are significantly dependent on inflow from rivers, streams and aquifers. TERRESTRIAL RESERVES The development of systems of representative freshwater reserves needs to be understood in light of the development of representative reserves in terrestrial and marine environments. The creation of terrestrial reserves preceded the creation of marine reserves by around one hundred years. Freshwater reserves, in their own right, have been an even more recent development 3. For most of the last century, terrestrial reserves were created for a variety of reasons, and were mostly established by ad hoc or opportunistic pressures. Even though Australia made an international commitment to the establishment of representative ecosystem reserves 20 years ago, it is only in the last 10 years that most nature conservation agencies have embraced the goal of representing the wide range of ecosystems within each jurisdiction in a system of protected areas. Within the Australian context, both Commonwealth and State governments are now firmly committed to the establishment of systems of representative terrestrial reserves, and these programs have now been funded for the best part of a decade. Given the slow start that these programs have had, it is understandable that priority has been given to planning at the regional and landscape level. However, these broad-scale programs are now sufficiently well established, we argue, for matters of finer detail to be considered - such as freshwater ecosystems. It is true that existing systems of terrestrial reserves protect many important freshwater ecosystems. Probably the most impressive example is provided by the World Heritage Area in the Southwest of 2 Tasmania, where the two most westerly of the State’s nine IBRA regions are substantially protected, including their small and medium-sized waterways (some of the larger waterways are degraded by hydroelectric developments). Similar comments regarding the protection of small rivers, lakes, wetlands and aquifers can be made for most other very large terrestrial reserves. Moreover, in some States (see the discussion of the South Australian program below) reserve acquisition programs are now targeting wetland acquisitions. MARINE RESERVES The development of marine reserves has lagged behind terrestrial reserves by about a century, partly due to the incorrect perception that the sea was so vast it seemed improbable that humans could cause significant long-term degradation. In addition, damage which was occurring was invisible to most of the community with the result that marine conservation issues remained lowprofile with both politicians and conservation lobby groups. National Representative System of Marine Protected Areas The development of the National Representative System of Marine Protected Areas (NRSMPAs) was endorsed by Australian Governments under the InterGovernmental Agreement on the Environment. There are commitments by all Australian Governments to its establishment in key strategies such as the National Strategy for Ecologically Sustainable Development (1992) and the National Strategy for the Conservation of Australia’s Biological Diversity (1996) [Australia's Oceans Policy 1998:Appendix 4]. Goals of the NRSMPA The primary goal of the NRSMPA4 is to establish and manage a comprehensive, adequate and representative system of MPAs to contribute to the long-term ecological viability of marine and estuarine systems, to maintain ecological processes and systems, and to protect Australia’s biological diversity at all levels. The following secondary goals are designed to be compatible with the primary goal: to promote the development of MPAs within the framework of integrated ecosystem management; to provide a formal management framework for a broad spectrum of human activities, including recreation, tourism, shipping and the use or extraction of resources, the impacts of which are compatible with the primary goal; to provide scientific reference sites; to provide for the special needs of rare, threatened or depleted species and threatened ecological communities; to provide for the conservation of special groups of organisms, eg species with complex habitat requirements or mobile or migratory species, or species vulnerable to disturbance which may depend on reservation for their conservation; to protect areas of high conservation value including those containing high species diversity, natural refugia for flora and fauna and centres of endemism; to provide for the recreational, aesthetic and cultural needs of indigenous and non-indigenous people. 3 IMCRA: an ecosystem-based regionalisation of Australia’s oceans The Interim Marine and Coastal Regionalisation for Australia (IMCRA) is an ecosystem-based classification of Australia's marine waters. It describes regions at the meso-scale and macro-scale, drawing on information about the biological, physical and chemical variability of the sea floor and overlying waters. As is the case with IBRA, IMCRA seeks to establish regions containing repeating patterns of similar ecosystems. Preliminary work on a macro-scale regionalisation of the exclusive economic zone and the continental shelf has also been completed. Regionalisations such as those used in IMCRA are conceived and developed for specific purposes. Ecologically based regionalisations provide the first layer in a broad ecological planning framework within which more detailed information on ecosystems, communities and/or species distributions can be used to assist decision-making across or within a region . Reference: [Australia's Oceans Policy 1998:Appendix 4]. Implications for the development of representative freshwater reserves There are obvious similarities between the objectives and processes of the NRSP and the NRSMPA, and the possible development of a national system of freshwater reserves. For example, given the fundamental similarities between management aims and processes relating to both marine and freshwater environments, the goal statements could, for the most part, be transferred directly from marine to freshwater. The processes in developing such systems also appear to have obvious parallels. Thackway (1996:5) describes five broad steps in the development of systems of representative terrestrial reserves: identifying gaps in the current system of protected areas and setting priorities using a bioregional planning framework; identifying representative ecosystem areas (ie. sites of key conservation significance); selecting potential reserve areas (ie. potential candidate protected areas); assessing the feasibility of potential reserve areas and negotiating new protected areas; and establishment and management (ie. implementation and acquisition). These same steps could, broadly, form the basis of a national strategy aimed at establish systems of representative freshwater reserves. WHY DO WE NEED REPRESENTATIVE FRESHWATER RESERVES? Australian freshwater ecosystems By way of national overview, Australia, by virtue of its size, contains a large variety of different freshwater ecosystems. Broadly, the north of the continent has a monsoonal rainfall pattern, while the south generally has a temperate, winter-rainfall pattern. In the far south, Tasmania (the smallest State) captures more than half of Australia’s total annual surface runoff. The eastern seaboard and the extreme south west of the continent are reasonably well-watered, while the arid interior is characterised by rainfall which is extremely variable. By world standards, Australia has only one large river system, the Murray-Darling, whose catchment drains the western slopes of the Great Dividing Range. The Murray-Darling Basin 4 covers an area in excess of a million square kilometres (over one sixth of the entire continent) and occupies large areas of southern Queensland, inland NSW, and northern Victoria, as well as South Australia's south east. The Murray-Darling is also one of Australian’s most degraded river basins, an issue of special concern to South Australia – the State at the “bottom end” of the basin catchment. Threatening processes Threats to freshwater ecosystems are discussed in a variety of references, including the 1996 State of the Environment Report (refer also to the Report's technical background papers), and wetland strategies and policies developed by States. Major problems affecting many remaining rivers, wetlands and aquifers includes the following (not in order of importance): extraction of surface or groundwater flows; alterations to flow volumes, timing, temperature and quality stemming from stream regulation (dams and weirs) and levee bank construction; water pollution from agricultural, urban and industrial sources; habitat degradation, including: (a) degradation of riparian vegetation by grazing and altered fire regimes, (b) weed infestation, (c) changed river morphology associated with erosion and sediment deposition, (d) recreational activity impacts, including destruction of vegetation, destructive litter such as nylon fishing lines, and poisoning of soils and biota with lead shot, (e) river de-snagging, and (f) drainage, infilling or channelisation for agricultural purposes; structures impeding the passage of fish on life-cycle journeys; introduced aquatic pests species (including plants, animals and micro-organisms); disturbance of wildlife by recreational and urban encroachment, powerlines (causing birdstrike) and predation by feral animals such as cats, foxes and dogs; and the direct and indirect effects of mining operations, including: (a) the mining of materials associated with water systems, such as peat, sphagnum moss, gold, certain gemstones, tin, sand and river gravel, (b) water pollution resulting from mining operations, especially sediment and acidic wastes, and (c) altered groundwater regimes resulting from mine dewatering or process water extraction. The role of representative freshwater reserves As is the case in terrestrial and marine environments, there are a number of roles that representative freshwater reserves could play. These include: protection of biodiversity against threatening processes through the establishment of a comprehensive, adequate and representative system of reserves; provision for the conservation of special groups of organisms – for example, species with complex habitat requirements, or mobile or migratory species, or species vulnerable to disturbance and which may depend on reservation for their conservation; provision for the special needs of rare, threatened or depleted species, and threatened ecological communities; provision of biodiversity ‘banks’ to recolonise damaged or degraded environment, whether such degradation has occurred by natural disaster, bad long-term management practices, or by accident; provision of scientific reference sites, either for research, or to provide benchmark indicators by which sustainable management may be judged; and protection of areas of high conservation value including those containing high species diversity, natural refugia for flora and fauna, and centres of species endemism; within the constraints of the above, provision for the recreational, aesthetic and cultural need of indigenous and non-indigenous people. 5 Protection of biodiversity All major biodiversity strategies worldwide are based on the principle of protecting biodiversity "in-situ". Biodiversity is generally defined at three levels: genes, species and ecosystems5. There are species which can be maintained by captive breeding programs, and to this extent these species, and their genes, could in theory be maintained without the existence of reserves protecting their habitat. These species represent, however, only a tiny fraction of the total number of species in existence. A huge proportion of invertebrate and microbial species remain unidentified and undescribed to science. Estimates of the total number of species in existence range from 10 million to 100 million. While most vertebrate and plant species have almost certainly been identified, full knowledge of their ecological requirements is available for comparatively few, and for most of these captive breeding programs are impractical or uneconomic. There are many species where captive breeding programs are impossible. At the third level of biodiversity, 'captive breeding' programs for entire ecosystems is impractical or uneconomic (if not impossible). Even attempts to create simplified ecosystems to support small numbers of humans (biodomes) have been unsuccessful, although conducted at great expense. A cornerstone of biodiversity protection (first articulated in the international context in the World Charter for Nature 1982) is the tenet that, where ecosystems are subject to significant modification by humans (through harvesting, pollution, resource extraction, or the introduction of new species, for example) it is necessary to set aside representative examples of these ecosystems to provide biodiversity “banks”, and benchmarks against which human management of the ecosystems can be measured in the long term. The “mirror” of this tenet states that actions should also be taken in managed ecosystems to minimise impact by protecting natural values (including biodiversity) as far as practicable. Threatening processes need to be identified and managed. Where reserves are created to protect representative ecosystems, such reserves should be ecologically viable. They should be large enough to support species at the top of the food chain, such as the peak predators, and should be of sufficient size to permit ongoing evolutionary processes to occur. In the words of the International Convention on Biological Diversity (UNEP 1992) they should be comprehensive, adequate and representative. The above cornerstone is one of the key foundations of the International Convention on Biological Diversity, and has been broadly adopted by all national biodiversity strategies developed by signatory-nations to the Convention, including Australia's strategy. The Australian biodiversity program was established by the National Strategy for the Conservation of Biological Diversity 1996, to which all Australian States are signatories. This is referred to below in shorthand form as the national biodiversity strategy. In summary, the development of systems of representative reserves, protecting viable examples of all major ecosystems, is fundamental to the conservation of biodiversity. Where samples of ecosystems cannot be viably protected in the long-term, biodiversity losses will inevitable occur, as human use of these ecosystems modifies and simplifies their characteristics. 6 Protection of other ecological values A recent publication by Phillips et al6 reviews existing principles and tools associated with the protection of the ecological values of rivers. Ecological value can be considered the natural significance of ecosystem structures and functions, expressed in terms of their quality, rarity and diversity. Significance can arise from individual biological, physical or chemical features or a combination of features. Dunn7 surveyed a range of river researchers/managers to determine the key criteria which define the aspects of rivers that should be protected. A summary of criteria identified by the survey recipients is listed below. rarity - what is the relative occurrence of river features? naturalness - how much has human occupation affected the river? diversity - what is the range of biological and physical features which define the river? representativeness - how well does the river reflect its type? special features - are there distinctive features of a river which require specific management? Effective river protection or conservation must involve both ‘conservationists’ and those who exploit natural resources, together with researchers, planners, educators and the general public8. There is very little direct protection of rivers being undertaken in Australia. This situation is not dissimilar to that in other countries. For example, Collier9 reported that, in New Zealand, conservation efforts had historically focussed largely on preserving fisheries values. Allan and Flecker10 claim that the strong global interest in biodiversity has concentrated efforts into ecosystems such as tropical moist forest, to the detriment of other systems such as aquatic environments, with perceived lower biodiversity levels. Dunn11 describes the current status of river protection in Australia, with mostly indirect protection being achieved through compliance with the COAG water reform framework agreed between State and Federal governments (this incorporates the National Water Quality Management Strategy12). This agreement commits all Australian jurisdictions to the protection of the aquatic environment, explicitly including the provision of water allocations for maintenance of these values - where they are flow-dependent. Protection of water quality is another indirect instrument for the protection of ecological values. Direct protection instruments, for example through protection of representative river ecosystems by special designation, are not generally applied. Historically, a good deal of river protection has been achieved as a secondary outcome of the need to prevent the erosion of farmland, or the need to implement sustainable land use practices etc (Penn, pers comm, Sep 2000). Provision of benchmarks Representative reserves provide benchmarks against which environmental changes in managed ecosystems can be measured. Benchmarks are also necessary to assess the value and condition of impaired ecosystems13. For example, the AusRivAS macroinvertebrate sampling program provides important benchmarks and indicators for the measurement of river condition. In the long term, such benchmarks may be altered by aspects such as climate change. Without reference areas, there will be no reference standard by which to judge the implications of such long term changes. Sustainable management programs cannot be validated without benchmarks. Without representative reserves, programs aimed at achieving sustainability become vacuous attempts at window-dressing. 7 COMMITMENTS, PROGRAMS AND DIFFICULTIES State commitments and programs: representative reserves The following table lists State commitments to the development of systems of representative freshwater reserves, and the programs developed to put these commitments in place (extracted from Nevill 2001). Summary tabulation: Representative freshwater reserve commitments and programs Commitment contained in: Implementation program Wetlands Conservation Policy 1997 None. WA The commitment has not been re-stated in the recent Waterways WA Policy,. A Strategy for Conservation of the None NT Biological Diversity of Wetlands, 2000 Wetlands Strategy 1999 None Qld None NSW Rivers and Estuaries Policy 1993; Wetlands Management Policy 1996; Biodiversity Strategy 1999; Nature Conservation ACT Nature Conservation Strategy 1998 Program Nature Conservation Strategy 1987; Heritage Rivers Vic Biodiversity strategy 1997 Program wetlands component incomplete Developing Tasmania’s nature conservation None Tas strategy; 2000 (- commitment in draft form). SA is developing a draft wetlands policy (no No commitment made SA commitment to representative freshwater reserves at this stage. at this stage). In summary: all States are committed to the national biodiversity strategy (where the development of representative reserves covering all ecosystems is a key commitment). All states, except South Australia, have amplified this comment by specific policy statements relating to the development of systems of representative freshwater reserves, although the Tasmanian commitment remains in draft form as of June 2001. All States have programs in place designed to meet Ramsar commitments - these commitments include the development of ecosystem inventories, and the establishment of systems of reserves covering the full range of wetlands included in the Ramsar definition of the term. In no case are these programs complete. The only jurisdiction to establish a reasonably comprehensive freshwater ecosystems inventory is the Australian Capital Territory, and the ACT is the only jurisdiction to establish a reasonably comprehensive system of freshwater reserves. The ACT and Victoria are, in fact, the only 8 jurisdictions to attempt to directly action their "representative reserve" commitments. The Victorian program, while ambitious, has not been completed, and is now in urgent need of review. In all other jurisdictions, action has not been taken to put in place either comprehensive inventories, or systems of representative freshwater reserves. Instead, these States have concentrated on the broader bioregional framework of the NRS, which itself has not highlighted the freshwater reserve issue. Moreover, no action has been taken within the NRS to establish a national agreed approach to the classification of freshwater ecosystems into categories or types which could provide a framework for the long-term development of a national system of representative freshwater reserves. Difficulties in managing aquatic reserves The fundamental element of representative reserve management is the separation, as far as possible, of the protected ecosystem from the processes which threaten it. The issue of how the catchment of a reserve might be protected provides an obvious complication with regard to freshwater reserves that does not generally apply to terrestrial or marine reserves14. The use of land and water upstream of the reserve will affect the viability of the reserve itself. The existence of downstream dams and weirs will inhibit or prevent fish passage. This aspect means that creation and management of the reserve must bear catchment issues in mind – and in some cases, seek to influence activities within the catchment in order to protect values of the reserve. This complication is just that: a complication. It does not imply that the concept of a representative freshwater reserve is somehow different in principle to a terrestrial or a marine reserve. The essence of all reserves is that boundaries can be drawn, and management plans and programs prepared, to effectively protect the target ecosystem. CONCLUSIONS It is clear that freshwater ecosystems are under increasing threat. Many of the threats are pervasive and intractable. Systems of terrestrial reserves have been established, and the largest of these, and those specifically targeted at wetland areas (such as Ramsar sites) undoubtably protect some representative samples of major freshwater ecosystems. However, in spite of international, national and State-level commitments to the establishment of representative systems freshwater reserves, only Victoria and the Australian Capital Territory have made serious attempts to establish such reserves. The Australian Capital Territory has inherent advantages due to its small size, and the large amount of public land within its jurisdiction, and here some impressive reserves have been created. Victoria led the nation with it's 1987 Nature Conservation Strategy, the subsequent Rivers and Streams Investigation by the Land Conservation Council, and the eventual passage of the Heritage Rivers Act 1992. However, the initial vision of the Victorian program has not been realised, and the issue is now in need of urgent review in that State (discussed above). Australia's remaining six jurisdictions have not moved to implement their commitments (Nevill 2001). This delay should be seen within the perspective of the need to establish the broader bioregional National Reserves System, which has occupied most Australian nature conservation agencies over the last decade. This has, by necessity, focussed attention at the bioregional and landscape level. Most freshwater ecosystems exist at a finer level of detail, and have so far escaped priority attention within the National Reserves System. It is time for this approach to change. Sufficient progress has been made at broad planning levels now to justify turning attention to ecosystems of finer detail - in particular, rivers, lakes, wetlands 9 and aquifers. Freshwater ecosystems should now be highlighted within the National Reserve System framework. No Australian State has met its full Ramsar Convention obligations in relation to the preparation of comprehensive wetland inventories, using the Ramsar definition of "wetlands" (Nevill 2001). Partial inventories have been established, and these are valuable. They must now be expanded, using nationally agreed classification methods, to encompass all major freshwater ecosystems. These inventories can then be used to identify gaps in the existing reserve system. It is to be expected that the most significant gaps, and those which will be most difficult to fill, will relate to large midland and lowland rivers, and possibly aquifers. Classification and assessment methods of potential relevance to the development of comprehensive freshwater inventories are set out in Appendix 4 of Nevill (2001). There will be obvious difficulties involved in management issues due to the dependence of freshwater ecosystems on the condition and management of the wider catchment; however, just because something is difficult does not mean that it can't be done. Successful implementation of national and State commitments to reserve systems rests on two fundamental premises. First, that Australia needs to establish a bioregional planning and management framework capable of operating across all scales and which is accepted by all sectors of government, industry and the wider community. Second, that in planning the National Reserve System it is essential to involve the wider community and all stakeholders earlier in the process of identifying and selecting areas for reservation. RECOMMENDATIONS No comprehensive evaluation has been carried out by any Australian jurisdiction to assess the degree to which freshwater ecosystems are in fact represented in existing reserves, it must be said that the commitments15 made by Australian State governments have not been met. We believe that Australian nature conservation programs are now at the point where consideration needs to be re-directed toward programs to protect ecosystems at a finer scale. Given the continuing and intractable decline of freshwater ecosystems over much of the Australian continent, it is now urgent that the development of representative freshwater reserves no be elevated, nationwide, to the highest priority. Our two central recommendations are that: agreed National methods for the classification of freshwater ecosystems into (‘representative’) categories should be established, which can be incorporated into a comprehensive national inventory; and a national approach should be developed to enable the identification of gaps in the existing reserve system relating specifically to freshwater ecosystems. These, we believe, should be initiated within the cooperative frameworks of the NRS and ANZECC, assisted by agencies such as AFFA, EA (wetlands program), the National Audit, and Land and Water Australia (including involvement by the National Rivers Consortium). REFERENCES Please refer to the ASL representative reserves bibliography at http://www.onlyoneplanet.com/ASL_bibliography.htm . 10 The terms “comprehensive, adequate and representative” were adopted by the International Convention on Biological Diversity (UNEP 1992), and have subsequently been incorporated into the National Reserves System Program, and the Marine Protected Area National Reserve System Program. 2 Ramsar Convention: at http://www.ramsar.org/ . 3 With the exception of rivers protected under the USA’s Wild and Scenic Rivers Act 1968. 4 Reference: [Guidelines for establishing the National Reserve System of Marine Protected Areas; ANZECC 1998:s1.3web]. 5 These levels can be expanded, however. Refer Commonwealth of Australia 1997b: 7. 6 Phillips, N. Bennett, J and Moulton D (2001) Principles and tools for protecting Australian rivers. Land and Water Australia. 1 7 Dunn, Helen (2000) Identifying and protecting rivers of high ecological value; LWRDDC Occasional Paper 01/00. Land and Water Resources Research and Development Corporation, Canberra 8 Boon P J and Baxter J M (1999) Issues in aquatic conservation: past, present and future. An introduction. Aquatic Conservation: Marine and Freshwater Ecology 9: 495 – 496. 9 Collier, K.J., (1993) Towards a Protocol for Assessing the Natural Value of New Zealand Rivers. Science and Research Series No.58 Department of Conservation, Wellington, New Zealand. 10 Allan, J.D.and Flecker, A.S. (1993) Biodiversity Conservation in Running Waters: Identifying the major factors that threaten destruction of riverine species and ecosystems. Bioscience, 43(1) 3243. 11 Dunn, Helen (2000) Identifying and protecting rivers of high ecological value; LWRDDC Occasional Paper 01/00. Land and Water Resources Research and Development Corporation, Canberra. 12 ARMCANZ & ANZECC (1994) 13 Reynoldson, TB; Norris, RH; Resh, VH; Day, KE; Rosenberg, DM (1997). The reference condition: a comparison of multimetric and multivariate approaches to assess water-quality impairment using benthic macroinvertebrates. Journal of the North American Benthological Society 16, 833-852. 14 With the obvious exception of marine reserves encompassing estuaries. 15 That is, commitments to establish representative systems of freshwater reserves. 11