STANDARD OPERATING PROCEDURE (SOP)
FOR MOUSE HUSBANDRY IN CHS B2-225
I.
Purpose and Scope:
The purpose of this document is to specify the procedures for care of _mice__
housed in _CHS B2-225__ under the care of __Dr. Larry Jones___.
II.
Responsibilities:
A. __Dr. Larry Jones___ will be responsible for reviewing and updating this
document annually or as needed.
B. All personnel will be responsible for reading and complying with the
provisions herein.
III.
General:
A. Species:
Mouse
B. Husbandry requirements
Temperature: 68°-79°F
Humidity: 30%-70%
Light Cycle: ON 6:00am/OFF 6:00pm
Room Pressure: Negative
C. Special needs:
Immunocompromised animals will require sterile cage set-ups.
D. Stabilization period: Newly received animals should be given a period for
physiologic, behavioral, and nutritional acclimation before their use. 72
hours is recommended.
IV.
Procedures:
A. Daily monitoring and care of animals (by whom; mechanism of
communication with the veterinarian by phone or pager regarding problems of
animal health, behavior, and well-being; weekend, emergency, and holiday
care)
Daily monitoring and care of animals (including weekend and holiday care)
will be performed by Jack Smith and Susan Lambert. Animal activities,
alertness, fur condition and breathing pattern will be monitored visually. In
case of problems of animal heath or behavior, the veterinary staff will be
notified by phone call or paging. The veterinary on-call pager number is
800-233-7231, pager #96545.
B. Feeding (what, amount, frequency, by whom, food storage, method for
monitoring milling or receipt date)
Food (Teklad 7013 Rodent chow) will be checked every morning by Jack
Smith, and food is stored in a sanitizable container which is labeled
Standard Operating Procedure (SOP)
Page 2
with milling date on top. Laboratory diets will be kept for 6 months
maximum after the milling date.
C. Watering (frequency, by whom, method of monitoring cleanliness and proper
operation of watering devices)
Water bottles will be checked every morning. Water and bottles will be
changed weekly by Jack Smith. Clean water bottles will be obtained
weekly from DLAM.
D. Bedding and cage changes (frequency, by whom, bedding storage)
Jack Smith will change bedding and cages weekly. Bedding will be
provided by the animal facility together with the clean cages, feeders,
and water bottles.
E. Sanitation of study area, cages or tanks, feeders, water bottles, food and
bedding storage containers, racks or shelves (frequency, solutions used, by
whom). Floors should be kept clean and free of debris. DLAM/EH&S
approved cleaners should be used. Please note that sanitation “as often as
needed” is not acceptable.
The study area will be cleaned every time before and after procedures
using antibacterial cleaner (e.g., Clidox, Power-Cide, or Virkon). Animal
racks will be cleaned weekly and food container monthly. Jack Smith is
responsible for the cleaning procedures.
F. Record-keeping (see sample study area log)
Records of animal care, morbidity/mortality, room maintenance and
environmental conditions are posted in the study area and updated
daily, including weekends and holidays, by Jack Smith or Susan
Lambert and submitted to the Office of Animal Research Oversight
(oaro@research.ucla.edu or fax #60742) on a monthly basis.
G. Transportation procedures, if applicable (i.e., between vivarium and lab)
A designated cart will be used to transport animals from vivarium to the
lab. The cages will be covered with solid sheet en route. No animals
will be transported back to vivarium.
H. Other (e.g., water and filter changes for aquatic species, water quality
testing).
None
I declare that I have been trained to recognize a sick or injured animal and that I will
report any ailments, injuries, or deaths to a veterinarian.
Signature of all personnel who will be responsible for reading and complying with the
provisions above:
X
Revised 10/25/13
X