RESUME
MARIANA FRANCO-RUIZ
OBJECTIVE
Information
EXPERIENCE
2006 to date: North Carolina State University. Department of
Horticultural Science. PhD student, Research Assistant (full
assistantship) Mapping origins of DNA replication in plants.
2004-2005: North Carolina State University. Crop Science
Department. Research scholar. Site-specific Recombination
System.
2002 to 2004: North Carolina State University. Botany
Department. Research Scholar. Site-specific Recombination System.
 Basic molecular biology techniques.
 Generation of transgenic plants (Arabidopsis) with different
reporter genes and target systems.
 Crosses of transgenic lines (Arabidopsis and Nicothiana
tobaccum)
 PCR
 Plant Tissue Culture (suspension cells, calli and calli
transformation)
 Plant DNA purification
 Plasmid DNA purification
 Plant RNA purification
 Nuclei purification from callae
 GUS Reporter gene detection
 Photon Imaging (Luciferase assay)
 GFP screening
 Selection and maintenance of Arabidopsis transgenic lines.
 Media preparation.
1999-2001
CINVESTAV (Center for Research and Advanced
Studies, Plant Genetic Engineering Department) Irapuato, Mexico
Thesis work and Lab Technician
 Bromatological Analysis of 3 different lines of Transgenic
Potatoes Engineered for Virus Resistance. (Fat, Protein,
Carbohydrates, Ashes and Fiber).

Protein profile of transgenic potato lines.

Plant Virus Maintenance (Inoculation and Purification).

Plant Virus Monitoring by ELISA and DAS-ELISA Procedures.


DNA bombardment (Gene gun) on pepper and tobacco plants
for plant viruses inoculation.
Viral DNA Purifications.

Plant DNA Purifications.
RNA and DNA Virus interactions assays (In planta)

Scientific Photography Course.

Greenhouse and Growth Chambers plant assays.

Arabidopsis inoculation and maintenance.


Diverse Molecular Biology Techniques: Dot Blot, Southern Blot,
Squash Blot, Gel electrophoresis (DNA and protein) etc.
1999
COFOCE (World Trade Promotion) Irapuato, Mexico.
Adviser
 Quality
Control and Product Facilities Improvement for
Product Export.
1994-1998
Instituto de Ciencias Agricolas (University of
Guanajuato) Irapuato, Mexico.
Dairy Production Manager
 Production.

Quality Control.

Sales.
1996-1997 GAB Inc. (Subsidiary of Doyle, Inc). Irapuato, Mexico.
Field Pest Control
 Field Epidemiology.

Insect Control Spraying Program.
1995-1996
CESAVEG (USDA equivalent in Mexico) Irapuato,
Mexico.
Entomology Lab, Quality Control Officer
 Biological Control Assays of parasitoid fungi against broccoli
beetles.

Microbiological Quality Control.

Bioinsecticide production.
EDUCATION
1994–1999
Universidad de Guanajuato (Instituto de Ciencias
Agricolas)
Irapuato, Guanajuato, Mexico.
 Food Engineering (Agriculture)


Laureated Thesis "Proximal and Protein Analysis of Alpha,
Norteña and Rosita transgenic potato lines transformed with
PVX and PVY coat protein".
Patent # 37CFR §1.51(c)(1) (submitted)
PUBLICATIONS
Kumar, S., M. Franco, and G. C. Allen. 2006. Gene targeting:
Development of Novel Systems for Genome Engineering in Plants.
In: Floriculture, Ornamental and Plant Biotechnology:
Advances and Topical Issues. Chapter 8, pp. 84-98. J. A.
Teixeira da Silva (ed). Global Science Books, London, UK
5116 –15B FT. SUMTER RD. * RALEIGH, NC 27606 * PHONE (919) 851-7407
E-MAIL: mayanab@hotmail.com; mfranco@ncsu.edu