The Laboratory for
Developmental Biology
DNA WORKSHEET
SERVICE REQUEST
Investigator ____________________________
Department ____________________________
Lab Contact ___________________________
Telephone _____________________________
Billing Contact _________________________
Billing Telephone _______________________
Billing Fax ____________________________
Gene Name (your designation)
IMM Abbreviated Name (leave blank)
DNA Prepared By (see instructions)
Method Used to Purify DNA (e.g. Gene Clean)
Mouse Strain (see instructions if other than ICR)
Making
Dilution
O.D. 260
Date _________________________________
Institution _____________________________
E-Mail ________________________________
Fax ___________________________________
Billing Address _________________________
______________________________________
______________________________________
______________________________________
______________________________________
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Transgenic Line __ Transient Transgenics __
Table: Fill out the first line only.
O.D. 280
260/280
CONCENTRATION
Picture: Run out one or two microliters of the DNA on a gel with a marker.
Place Picture Here
Fill this form out and mail with the DNA fragment OR
fax the forms & mail or deliver the DNA fragment to:
Laboratory for Developmental Biology; Institute of Molecular Medicine,
Suite 911B, 2121 W. Holcombe Blvd., Houston, TX 77030
Telephone: (713) 500-2452, Fax: (713) 500-2424, E-Mail: transgenic@uth.tmc.edu
INSTRUCTIONS
DNA Prepared By: The requirements for an acceptable linearized DNA preparation
are:
(1) 260/280 of 1.7 to 2.0
(2) Concentration of 0.1 to 0.2 micrograms/microliter
(3) Volume of 30 to 50 microliters
(4) Measure O.D. at 1:100 dilution
Mouse Strain: Specify mouse strain needed as background strain. This need only be
filled out if strain required is other than the standard ICR outbreed strain.
Making: Check one, usually Transgenic Line. Transient Transgenics are made when the
investigator wants offspring prior to birth for developmental studies or if there is
embryonic lethality.