pmic7215-sup-0001-TableS1

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Table S1. Table showing various significant QDs in proteomic applications
QD
CdSe-ZnS
QDs.
Protein analytes
Application
Methods
Ref.
histidine, cysteine, methionine, and tryptophan
[1]
oligohistidine fused proteins
[2]
thrombin
Flourescence
[3-5]
recombinant proteins
[6]
InGaP/ZnS
bovine serum albumin (BSA)
[7]
CdSe QDs
(Telomere associated proteins telomeric repeat
binding factor (TRF1, a 56 kDa protein) and TRF1interacting nuclear protein 2 (Tin2,40 kDa protein)
directly in cell lysate
[8]
QDs western blot
CdTe QDs
protein A
[9]
CdSe/ZnS
QDs
carcinoembryonic antigen CEA
[10]
CdSe/ZnS
QDs
TNF-α, IL-8, IL-6, MIP-1β, IL-13 and IL-1β
CdSe/ZnS
prostate specific antigens (PSAs)
Protein chip
[11]
Cancer protein
chip
[13]
Insulin
CdS QDs
Protein and peptides
[12]
Mass
spectrometry
Cytochrome, HSA, BSA.
[14]
[15]
References
1. Peelle, B. R.; Krauland, E. M.; Wittrup, K. D.; Belcher, A. M. Design Criteria for
Engineering Inorganic Material- Specific Peptides. Langmuir 2005, 21, 6929–6933.
2. Sapsford, K. E.; Pons, T.; Medintz, I. L.; Higashiya, S.; Brunel, F. M.; Dawson, P. E.;
Mattoussi, H. Kinetics of Metal-Affinity Driven Self-Assembly between Proteins or
Peptides and CdSe−ZnS Quantum Dots. J. Phys.Chem. C 2007, 111, 11528–11538.
3. Levy, M., Cater, S.F., Ellington, A.D., Quantum dot aptamer beacons for the detection of
proteins. Chembiochem 2005, 6, 2163–2166.
4. Swain, M.D., Octain, J., Benson, D.E., Unimolecular, Soluble Semiconductor
Nanoparticle-Based Biosensors for Thrombin Using Charge/Electron Transfer.
Bioconjugate Chem 2008, 19, 2520–2526.
5. Chi, C.W., Lao, Y.H., Li, Y.S., Chen,L.-C., A quantum dot-aptamer beacon using a DNA
intercalating dye as the FRET reporter: Application to label-free thrombin detection,
Biosens. Bioelectron. 2011, 26, 3346–3352.
6. Mattoussi, H., Mauro, J.M., Goldman, E.R., Anderson, G.P., Sundar, V.C, Mikulec, F
.V., Bawendi,M.G., Self-Assembly of CdSe-ZnS Quantum Dot Bioconjugates Using an
Engineered Recombinant Protein. J. Am. Chem. Soc 2000, 122, 12142-12150.
7. Kumar, P., Deep, A., Sharma, S.C., Bharadwaj, L.M., Bioconjugation of InGaP quantum
dots for molecular sensing. Anal Biochem 2012, 421, 285–290.
8. Ornberg, R.L., Harper, T.F., Liu, H., Western blot analysis with quantum dot
fluorescence technology: a sensitive and quantitative method for multiplexed proteomics.
Nat Methods. 2005, 2,79-81.
9. Bakalova, R., Zhelev, Z., Ohba, H., Baba, Y., Quantum Dot-Based Western Blot
Technology for Ultrasensitive Detection of Tracer Proteins. J. Am. Chem. Soc 2005,127,
9328-9329.
10. Xu, Y., Gang, Bao, B., A Filtration-Based Protein Microarray Technique. Anal. Chem
2003, 75, 5345-5351.
11. Hu, M., Yan, J., He, Y., Lu, H., Weng, L., Song, S., Fan, C., Wang, L., Ultrasensitive,
Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum
Dot-Based Microfluidic Protein Chip. ACS Nano 2010, 4,488–494.
12. Jin, L.H., Li, S.M., Cho, Y.H., Enhanced detection sensitivity of pegylated CdSe/ZnS
quantum dots-based prostate cancer biomarkers by surface plasmon-coupled emission.
Biosens. Bioelectron. 2012, 33, 284– 287.
13. Shrivas, K., Kailasa, S.K, Wu, H.F., Quantum dots laser desorption/ionization MS:
Multifunctional CdSe quantum dots as the matrix, concentrating probes and acceleration
for microwave enzymatic digestion for peptide analysis and high resolution detection of
proteins in a linear MALDI-TOF MS, Proteomics 2009, 9, 2656–2667.
14. Wu, H. F., Chung, F.T., 3-Mercaptopropionic acid modified ZnSe quantum dots as the
matrix for direct surface assisted laser desorption/ionization mass spectrometric analysis
of peptides/proteins from sodium salt solution. Rapid Commun. Mass Spectrom. 2011,
25, 1779–1786.
15. Ke, Y., Kailasa, S. K., Wu, H. F.,Chen, Z. Y., High resolution detection of high mass
proteins up to 80,000 Da via multifunctional CdS quantum dots in laser
desorption/ionization mass spectrometry. Talanta, 2010, 83, 178–184.
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