Journal name: Molecular Breeding
Novel candidate genes AuxRP and Hsp90 influence the chip color of potato tubers
Dorota Sołtys-Kalina1*, Katarzyna Szajko1, Izabela Sierocka2, Jadwiga Śliwka1, Danuta StrzelczykŻyta1, Iwona Wasilewicz-Flis1, Henryka Jakuczun1, Zofia Szweykowska-Kulinska2, Waldemar
Marczewski1*
Plant Breeding and Acclimatization Institute – National Research Institute, Młochów, Platanowa 19,
05-831 Młochów, Poland
2
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of
Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
*Corresponding authors: D. Sołtys-Kalina; d.soltys@ihar.edu.pl; +48 22 7299248 ext. 218; fax: +48
22 7299247
W. Marczewski: w.marczewski@ihar.edu.pl: +48 22 7299248 ext. 215; fax: +48 22 7299247
1
Supplementary Table 3 Candidate genes obtained from the RDA-cDNA experiment performed in
two directions with cDNA from genotypes with a light chip color as a TESTER (LII) or as DRIVER
(DII) after cold storage (CS)
Direction of the
RDA-cDNA
experiment
LII
DII
References of cloned amplicons
PGSC assignment*
Chromosome
Conserved gene of unknown function
Nuclear RNA binding protein
Peptidyl-prolyl cis-trans isomerase
Hypothetical gene of unknown function
DEAD-box ATP-dependent RNA
helicase 52
Mediator complex, subunit Med11
domain containing protein
60S ribosomal protein L10
Heat shock protein 90
40S ribosomal protein S15
LeArcA1 protein
ATP synthase epsilon subunit 1
Vesicle-associated membrane protein
Zinc ion binding protein
Metallocarboxypeptidase inhibitor
Methallothioneine
Hypothetical gene of unknown function
8 sequences not assigned to any gene in
the potato genome
PGSC0003DMT400066818
PGSC0003DMT400066877
PGSC0003DMT400004125
PGSC0003DMT400093052
PGSC0003DMT400002828
I
I
I
II
III
PGSC0003DMT400060560
IV
PGSC0003DMT400053166
PGSC0003DMT400074377
PGSC0003DMT400042076
PGSC0003DMT400074094
PGSC0003DMT400068159
PGSC0003DMT400067225
PGSC0003DMT400079854
PGSC0003DMT400015767
PGSC0003DMT400023016
PGSC0003DMT400090811
Glycine rich RNA binding protein
Drm3
DNA-damage-repair/toleration protein
DRT111, chloroplastic
FRIGIDA
Heat shock protein 90
Ribosomal protein L28
Elongation factor 1-alpha
Gibberellin 3-oxidase/ Ribosome
biogenesis protein nop10
Metallothioneine
8 sequences not assigned to any gene in
the potato genome
PGSC0003DMT400001879
PGSC0003DMT400063589
PGSC0003DMT400054608
VI
VI
VI
VI
VI
VI
VII
VII
IX
X
6seq-II
1 seq-VIII
1seq-XII
I
I
II
PGSC0003DMT400084922
PGSC0003DMT400074377
PGSC0003DMT400012340
PGSC0003DMT400053547
PGSC0003DMT400042582/
PGSC0003DMT400065551
PGSC0003DMT400023016
VI
VI
VI
VI
VI
IX
2 seq-I
5 seq-II
1seq-IX
* assignment of cloned amplicons to genes on the potato genome was verified using Genome Browser
v4.03 available at the Potato Genomics Resource (PGSC) platform