Additional material to read


Purification strategies manual
Brief history of protein purification
pg 13-15 (optional)
Affinity chromatography
pg 17-18, 20
Gel filtration
pg 21-22
Ion exchange chromatography
pg 23-24
Hydrophobic interaction chromatography
pg 25-26
Reverse phase chromatography
pg28-29
Desalting buffer exchange
pg 31, 34, 36
Purification strategies
pg 39-4
Handbook of recombinant protein
Choice of host for protein purification

pg 6-8
Vectors for the Expression of Recombinant Proteins in E. coli, Sally A. Cantrell
http://mfa.od.ua/page175.htm
Protein purification manual, supplementary information.