Junior Biotechnology Syllabus 2011-2012
Week
Topic
24-Aug Welcome; Safety
S.L.O.P. - What is biotech,
Scientific Method,
Notebook, Business
29-Aug Model
S.L.O.P. - What is biotech,
Scientific Method,
Notebook, Business
6-Sep Model
Details
Related
section (s)
Welcome to Xenia High School, Info sheet and
allergies, eye wear, lab coats, Notebooks, book
assignments, HOSA Information, Apply for
admission at Sinclair, Biotechnology Program
Information and classroom management policies;
online safety modules; PPE; HHMI Safety Series
Safety book
S.L.O.P., Setting up a Lab Notebook, Experimental
Design, Cheese Lab and Cheese Lab Alterations
1.1, 1.2, 1.4
Stock Project, Biotech and Health Occupations
Poster HOSA Competition Rules; Bioethics
1.3, 1.5, 1.6
9-Sep
Unit 1 Test : Lab and Notebook Policy, Scientific Method, and Business Model
S.L.O.P. - Volume, Mass,
Measurement, Solutions, Volume, Mass and Measurement Techniques,
12-Sep Dilutions
Micropipetting
3.1, 3.2
S.L.O.P. - Volume, Mass,
Measurement, Solutions, pH, Solutions and Dilutions; M/V, %, Moles and
3.3, 3.4, 3.5,
19-Sep Dilutions
Molarity
3.6, 7.2
23-Sep
Unit 2 Test: Volume, Mass, Measurement, Solutions and Dilutions
S.L.O.P. - Making Media,
26-Sep Cell Culture, Sterilization
Making Media and Cell Culture; buffers
S.L.O.P. - Making Media,
3-Oct Cell Culture, Sterilization
Sterilization and Aseptic Technique
7-Oct
Unit 3 Test: Making Media, Cell Culturing and Aseptic Technique
7.3, 4.2, Lab
4a, Lab 4e,
Lab 4f, Lab
4g, (Black) CH
12
10-Oct Microbiology
Technique, Taxonomy, and morphology and
metabolism
2.1, 2.2,
(Black) CH 1,
CH 6
17-Oct Microbiology
Microscopic measurement
(Black) CH 3
24-Oct Microbiology
Culturing Specimens and Staining
(Black) CH 3
28-Oct
Unit 4 Test: Technique, morphology, metabolism, culturing and staining
End of 1st Quarter
31-Oct Nucleic Acids
DNA and RNA
2.3, 2.4, 4.1
7-Nov Nucleic Acids
14-Nov Nucleic Acids
DNA Extraction; Animal
DNA Extraction; Bacterial and yeast
4.3, 8.1
4.3, 8.1
18-Nov
Unit 5 Test: DNA, RNA, Extraction and Replication
21-Nov Capstone Project/Flex
28-Nov DNA Manipulation
5-Dec DNA Manipulation
Scientifically communicate findings discovered during the capstone
process.
Restriction Enzymes and recombinant DNA
4.3, 8.2, 6.5
DNA Cleavage
8.2, 6.5
12-Dec DNA Manipulation
Horizontal Gel Electrophoresis
4.4,
16-Dec
Unit 6 Test: Restriction enzymes, rDNA technology, gel electrophoresis
19-Dec Capstone/Flex
3-Jan DNA Technology
9-Jan DNA Technology
13-Jan
Scientifically communicate findings discovered during the capstone
process.
8.1, 13.1,
PCR Project, Using PCR to Amplify DNA
13.2
13.3, 13.4,
DNA Typing by PCR; Sequencing
14.1
Unit 7 Test: PCR, DNA synthesis, DNA enzymes
End of 2nd Quarter
18-Jan DNA Technology
23-Jan DNA Technology
Transformation; Lab 8c Transformation with
pAmylase
Transformation; pGLO Bacterial Transformation kit
30-Jan DNA Technology
Transformation; GFP Purification Kit
3-Feb
6-Feb Proteins (I)
8.1, 8.2,8.3
8.1, 8.2, 8.4
8.1, 8.2, 8.5,
9.3
Unit 8 Test: Recombination and Transformation
Structure and Function; 3D Molecular Design Kits
2.3, 5.1, 5.2
13-Feb Proteins (II)
Enzyme Function and Assays
5.3, 6.2
21-Feb Proteins (II)
Protein Assays and assay development
6.1, 6.2, 6.3
24-Feb
Unit 9 Test: Structure and Function, Amino Acid Characteristics, and Assays
27-Feb Proteins (III)
Vertical Gel Electrophoresis; PAGE
5.4,
5-Mar Proteins (III)
Vertical Gel Electrophoresis; PAGE
5.4,
12-Mar Proteins (III)
16-Mar
Vertical Gel Electrophoresis; PAGE
Unit 10 Test: PAGE and Molecular Weight of Proteins
5.4,
End of 3rd Quarter
19-Mar Proteins (IV)
Spectroscopy and Chromatography
26-Mar Proteins (IV)
Spectroscopy and Chromatography
30-Mar
2-Apr Immunology
7.1, 7.4
9.1, 9.2, 9.4,
9.5
Unit 11 Test: Spectroscopy and Chromatography
Immune System
Microbiology
(Black) CH 17
and 18
10-Apr Immunology
Antigen/Antibody Interaction
12.4
16-Apr Immunology
ELISA
14.3
Immunology - Diagnosis
23-Apr Doctor
27-Apr
30-Apr Forensics
Project: Diagnosis "You are the Doctor"
Unit 12 Test: Immunology
Training Labs: Classifying Fingerprints, problem
solving,lifting fingerprints, analyzing handwriting,
shoe prints for height and identification
7-May Forensics
Crime Scene Case of the Missing Tamarin Monkey
14-May Forensics
BioRad Crime Scene Investigator PCR Basics Kit,
Gazette Article about the Crime
18-May
End of 4th Quarter
23-May XHS Graduation
24-May GCCC Recognition Night for Seniors
Unit 13 Test: Forensics
Supplemental
material
Supplemental
material
Supplemental
material
13.4