Name __________________________________________________________
Test 1 BIO 304 Summer 2011
1
Name __________________________________________________________
Fill-in-the-blanks (2 pts. each). Write the letter corresponding to the word in the list on the right
that best fits in the blank in each sentence.
1. If you crossed a Chlamydomonas strain with the genotype D
E to one with the genotype d e, a tetrad that had the four
meiotic products D E, D e, d E and d e would be called a ____
tetrad.
2. If you crossed a Chlamydomonas strain with the genotype D
E to one with the genotype d e, a tetrad that had four meiotic
products D e, D e, d E and d E would be the result of a ____
during meiosis I.
3. Before S phase of meiosis, each chromosome is a single
____.
4. The probability that a couple who are carriers for an
autosomal recessive disorder have 3 affected and 4 unaffected
children can be calculated using ____.
5. If a dominant trait shows reduced ____, then it is possible
for an individual with a dominant allele to show the recessive
phenotype.
6. _____ is the phenotypic ratio predicted for progeny of a
dihybrid cross when the alleles of both genes show
codominance.
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
1:2:1:2:4:2:1:2:1
9:3:3:1
annealing
binomial probability
bivalent
chromatid
denaturation
dominant lethal
expressivity
four-strand double crossover
nonparental ditype
parental ditype
penetrance
product law
pUC19
recessive lethal
recombinant vector
sex-limited
synapse
sum law
tetratype
three-strand double crossover
two strand double crossover
7. In a PCR reaction, the temperature you chose to use for the ____ of the primer to the template
will be based on the length and GC content of the primer.
8. If a ____ occurs between linked genes during prophase of meiosis I, then the gametes will be
50% parental and 50% recombinant.
9. If you ligated chromosomal restriction fragments into pUC19, transformed the ligation into E.
coli, and plated the transformants on L+Amp+X-gal+IPTG medium, then white colonies would
correspond to transformants that received _____.
10. If a dihybrid cross produces 2/3 progeny with the dominant trait and 1/3 progeny with the
recessive trait, and all the dominant progeny are heterozygotes, then the dominant allele is said to
be a _____ allele.
2
Name __________________________________________________________
Concept Map. Write a phrase that makes a complete sentence which accurately describes the
relationship between the key terms at the corner of the box. There are 8 total phrases needed,
and each phrase must be written to clearly correspond to the appropriate arrow on the box. If the
arrow goes from “cables” to “bridges” then the phrase should read: (Cables) are components of
some (Bridges). The reverse arrow could read (Bridges) sometimes contain or use (Cables). Be
concise and accurate and you will earn 2 points for each arrow, with 2 points being provided for
demonstrating a reasonable effort to answer all 8 arrows.
dominance
9:3:3:1
alleles
epistasis
3
Name __________________________________________________________
Problem 1. Two alternative restriction maps of a
HindIII
HindIII
HindIII
3 kb
2 kb
R1
region on the Drosophila melanogaster
HindIII
HindIII
chromosome 3 are shown in the figure. The top
R2
map shows the map of the chromosomes in a
true-breeding (pure-breeding) strain that carries the recessive, X-linked, white allele. The bottom
map shows the map of the chromosomes in a true breeding (pure-breeding) strain with wild-type,
red eyes. Segregation of this region of chromosome 3 can be followed in crosses as a Restriction
Fragment Length Polymorphism (RFLP).
a) (8 Points) Indicate the eye and RLFP phenotype of the F1 and F2 of a cross between a whiteeyed female and a red eyed male.
TIP: Name the RFLP alleles R1 and R2 as shown in the figure.
F1:
F2:
male
ladder parent
5 kb
b) (2 Points) Fill in the phenotype for the RFLP locus in the F1 using
the gel to the right..
3 kb
2 kb
4
female
parent
F1
Name __________________________________________________________
B
A
Problem 2. The figure shows a
developmental pathway
Colorless
Dark
Light
controlling pigment synthesis in
a plant. The letters refer to two, autosomal genes controlling pigment synthesis, A and B. A and
B are dominant, functional alleles. a and b are recessive, loss of function alleles.
A pure-breeding colorless individual is crossed to a pure-breeding dark individual and produces
all light-colored F1. Use the model above and this information to determine:
a) the genotype of the parents
b) the genotype and phenotype of the F1
c) the F2 phenotypes and their proportions
5
Name __________________________________________________________
Problem 3. white, yellow and echinus are X-linked genes in a new species of Drosophila. Flies
with at least one w+ allele have red eyes whereas homozygous or hemizygous w- mutants have
white eyes. Flies with at least one y+ allele have gray bodies whereas homozygous or
hemizygous y- mutants have yellow bodies. Flies with at least one ec+ allele have normal shaped
eyes whereas homozygous or hemizygous ec- mutants have echinus-shaped eyes. A white,
echinus-eyed female was mated to a yellow-bodied male to produce an F1 generation. A female
from this F1 was mated to a white, echinus, yellow male to produce an F2. The number and
types of 10,000 total F2 are shown below.
a) (8 Points) Use this information to draw a map of the X chromosome in this new Drosophila
species that indicates the genetic distance between each pair of genes.
b) (2 Pionts)determine the value for interference in this region.
F2:
white, yellow, echinus
980
wild type
1000
white, yellow
8
echinus
12
white
500
yellow, echinus
480
yellow,
3620
white, echinus
3400
6
Name __________________________________________________________
Problem 4. Four possible restriction maps of a chromosome are shown in the figure (map A, B,
C, and D).
a) (5 Points) Use the restriction digest results for EcoRI, BamHI, and HindIII to determine which
map is correct.
b) (3 Points) Fill in the expected results for a digest with both EcoRI and BamHI.
c) (2 Points) Use the result of the EcoRI + SalI digest to draw the location of the SalI site on the
correct map.
EcoRI EcoRI
+
+
BamHI
BamHI
HindIII
SalI
EcoRI
EcoRI
15 kb
BamHI
HindIII
4
2
1
14 kb
13 kb
EcoRI
BamHI
EcoRI
5
12 kb
A
B
11 kb
10 kb
9 kb
EcoRI
EcoRI
HindIII
3
8 kb
C
7 kb
6 kb
BamHI
5 kb
4 kb
3 kb
2 kb
1 kb
7
EcoRI
HindIII
D