Australian BioResources SPERM CRYOPRESERVATION REQUEST FORM All fields in this form must be completed, indicate N/A if not applicable Please email this form to k.taylor@garvan.org.au 1. PERSONAL INFORMATION Date of Request Owner Principal Investigator Contact Name Email Address Phone Number AEC Approval # IBC Approval # 2. SUPPLIERS INFORMATION Name of Facility Contact Name Email Address Phone Number Does an MTA cover this line? Have you checked if freezing is allowed under the MTA 3. STRAIN INFORMATION Strain Name Background Strain Animal ID’s Recommended for successful archival of lines 4 fertile males Ideally proven breeders, 10-16 weeks old 1 Australian BioResources 4. FREEZING INSTRUCTIONS Thaw sperm, IVF and culture embryos to blastocyst. Desired validation method Thaw, IVF, transfer embryos into recipient mice for development to live pups. (Costs of respective options listed on page 2) Genotyping of recovered pups (if applicable) Tissue sample processed at ABR ad genotyped at ACRF (additional charges apply) Tissue collected and sent to owner (please specify required tissue) Please note: After weaning, housing costs of recovered pups are the responsibility of the owner. Costs Cost of freezing, with validation by culture $725.09 (partners) $833.85 (externals) Cost of freezing, with validation by live pup birth $1417.94 (partners) $1630.64 (externals) Annual cost of holding frozen sperm $25 per line ($20 per line if more than 4 lines are stored) I agree to pay the costs as indicated above. Name: ___________________________________________ Signature: ___________________________________________ Date: ______________________ Purchase Order No. (if applicable) ___________________________ Please direct any queries to Kevin Taylor (k.taylor@garvan.org.au). Tel: (02) 9295 8596 Fax: (02) 9295 8566 2 Australian BioResources For ABR use only: Programme / Schedule: Projected Program Start Date: ____________________ Completion Date: ______________________________ 3