TITLE
Development and Validation of RP-HPLC Method for the
Simultaneous Estimation of Azithromycin and Levofloxacin in the
combined tablet dosage form.
NAME:G.PRANATHI
ANURAG GROUP OF INSTITUTIONS,VENKATAPUR,GHATKESAR.
ABSTRACT
A simple, rapid, precise and highly selective spectrophotometric method
was developed for simultaneous estimation of Azithromycin and levofloxacin in
c o m b i n e d tablet dosage form. This method, involves the measurement of
absorbances of azithromycin and levofloxacin at the wavelengths of 230nm (λmax
of azithromycin) and 238 nm (λmax of levofloxacin). Distilled water was used as
solvent. Linearity was observed in the concentration range of 2-20µg/ml for both
azithromycin and levofloxacin. The accuracy of the method was confirmed by
recovery studies of tablet dosage forms and was found to be 0.09 and 0.06 for
azithromycin
and
levofloxacin
respectively.
The
method
showed
good
reproducibility and recovery with % RSD less than 2. The LOD of azithromycin
and levofloxacin was azithromycin and levofloxacin was found to be 20.50µg/ml
and 18.25µg/ml respectively. Thus the proposed method was found to be rapid,
specific, precise, accurate and cost effective quality control tool for the routine
analysis of azithromycin and levofloxacin in bulk and combined dosage form.s
found to be 6.134µg/ml and 5.474µg/ml and LOQ of azithromycin and
levofloxacin was found to be 20.50µg/ml and 18.25µg/ml respectively. Thus the
proposed method was found to be rapid, specific, precise, accurate and cost
effective quality control tool for the routine analysis of azithromycin and
levofloxacin in bulk and combined dosage form.
A selective, precise, isocratic and accurate stability indicating reverse
phase high performance liquid chromatographic method was developed for the
simultaneous determination of azithromycin and levofloxacin in the combined
tablet dosage form. A chromatographic separation was achieved on reverse phase
RP C18 column (250 ×4.6 mm, 5µ). The mobile phase consists of mixture of 0.1%
v/v Diethyelenepotassiumhydrogenphosphate methanaol and water(60;20;20).
The flow rate was 1ml/min and the effluents were monitored.The retention times
of azithromycin and levofloxacin were found to be 2.3min and 4.3min respectively.
The method was validated for the linearity, accuracy, precision, robustness,
system suitability as per ICH guidelines. Azithromycin and levofloxacin were found
to be linear in the range of 100-1000ng/ml with the recoveries of 100.01% and
99.01%.
BIOGRAPHY
G.Pranathi persuing M.pharmacy in Anurag group of institutions formly
(lalitha college of pharmacy) as it is an autonoumous university affiliated to
Jawaharlal Nehru technological university of Hyderabad.
PRESENTATION AUTHOR DETAILS
NAME:G.Pranathi.
CONTACTNUMBER:9603998127.
CATEGORY:POSTER PRESENTATION.