School of Science
Paisley Campus
Session 2011 – 12
Trimester 2
Module Code: BIOL09020
PURE AND APPLIED GENETICS
Date: May 2012
Time:
Duration:
Answer THREE questions, at least ONE from each Section
Page 1 of 4
Continued overleaf
BIOL09020
May 2012
Section A
Answer at least one question
1.
a) Discuss the features of the plasmid pBR322 (Figure 1) which
makes it a good vector for cloning DNA in bacterial cells.
b) Describe the steps you would perform to clone a DNA
fragment containing BamHI restriction sites at either end in
E.coli using the vector in Figure 1.
Figure 1
Page 2 of 4
Continued overleaf
(6)
(14)
BIOL09020
May 2012
2.
Write an account of the effects of genomic mutations with
reference to specific diseases.
(20)
3.
a) A DNA library is a useful resource for a scientist. Describe
the method(s) that would be used to create:
(8)
i) a genomic library and
ii) a cDNA library
b) Compare and contrast the content of the two libraries.
(6)
c) Describe a method to select a specific fragment of DNA with
a known sequence from a DNA library.
(6)
Page 3 of 4
Continued overleaf
BIOL09020
May 2012
Section B
Answer at least one question
4.
Outline the mechanisms of DNA replication and repair.
5.
A protein, G26, has been isolated from cells. The presence of
this protein in cells results in the increased expression of gene
Z. Structural studies have determined that this protein has a zinc
finger motif.
a) Discuss possible role of the protein, G26, in the cell.
6.
(20)
(4)
b) Describe experiments you would perform to gain more
information about this protein, G26, and its interaction
with DNA.
(16)
a) Human genomic DNA is tightly packaged as chromatin in the
nucleus of the cell. Discuss the evidence and proposals on
how gene expression can be influenced by the structure of
chromatin.
(10)
b) Discuss the events that occur at the level of transcription in
E.coli in response to the levels of tryptophan.
(10)
END OF QUESTION PAPER
Page 4 of 4