FEASIBILITY STUDY: RE-ESTABLISHING NATIVE FISH FAUNA TO MCKITTRICK CREEK, GUADALUPE MOUNTAINS NATIONAL PARK, TEXAS TX T-81-R-1 (F13AF00767) Interim Report October 2015 New Mexico State University Texas Parks and Wildlife Department Narrative During the reporting year (1 October 2014 – 30 September 2015), aquatic macroinvertebrates were identified to the lowest taxa. A total of 27 taxa was collected throughout both Fall 2013 and Spring 2014 sample collections across the four study reaches. Of this total, 85% were insects and included mayflies (Ephemeroptera - three taxa), caddisflies (Trichoptera - eight taxa), true flies (Diptera - six taxa), damselflies and dragonflies (Odonata - two taxa), true bugs (Hemiptera - one taxon), beetles (Coleoptera two taxa) and moths (Lepidoptera – one taxon). The remaining organisms (15%) were non-insect invertebrates and included snails (Mollusca - 1 taxon), segmented worms (Annelida - 1 taxon), water mite (Hydrachnida - one taxon), and scuds or side-swimmer (Amphipoda - one taxon). Average total standing crop (includes insect and non-insect taxa) decreased between Fall 2013 (20.8 organisms/m2 ± standard error 11.79) and Spring 2014 (14.6 organisms/m2 ± 5.14). Total standing crop was dominated in the Fall samples by the Dipteran Simulim sp. in contrast to the spring samples which were dominated by Chironomidae (midges). Taxa richness increased between Fall 2014 (5.9 ± 1.07) and Spring 2015 (9.0 ± 1.21) as a result of Leptophlebiids (Ephemeroptera) and Polycentropids (Trichoptera). Four taxa within the Diptera and Trichoptera groups were submitted for verification to lowest taxa and will be incorporated into an Appendix in the final report. Seasonal differences in standing crop and taxa richness will be analyzed and compared to earlier reports of benthic fauna in McKittrick Creek. Temperature dataloggers were deployed 5 November 2013 and temperature data downloaded 11 June 2014 during the Spring collection. A largescale flooding event occurred 22 September 2014 resulting in the loss of all air and temperature dataloggers. New ProV2 temperature loggers were deployed 21-23 November 2014 throughout all study reaches. Upon return to McKittrick Canyon to retrieve the temperature dataloggers (3 September 2015), all but one datalogger (Reach 3) were lost. However, no largescale flooding event was reported by Park personnel (J. Hearst, GUMO, personal communication). Throughout the 247 days logged in Reach 3, the maximum hourly water temperature was 29.5oC in late July 2015 (Table 1). The logger also recorded a maximum 24 h average temperature of 25.1oC and a maximum weekly average of 24.2oC in late July (Table 1). Note the red line across Figures 1-3 indicating the upper lethal limits for fry (24.7oC) and juvenile (23.4oC) Rio Grande cutthroat trout (Zeigler et al. 2013). Of the 144 rainbow trout captured Spring of 2014, 34% were represented by young-of-the-year (60-80 mm) and 41% were represented by year 1 (80-100 mm) age class indicating good reproduction and recruitment into the rainbow trout population (Figure 3). A largescale storm flow event occurred between Spring 2014 and Fall 2014 and presumably resulted in the decrease in rainbow trout abundance throughout all reaches (Table 2). Figure1. South McKittrick (Reach 3) hourly water temperature. Note missing data due to flooding event Fall 2014. The bold red line approximately reflects upper lethal limits for fry (24.7oC) and juvenile (23.4oC) Rio Grande cutthroat trout (Zeigler et al. 2013). Figure 2. South McKittrick (Reach 3) daily water temperature. Maximum daily, average daily, and minimum daily are presented in the top panel and the daily range is represented in the bottom panel. Daily temperatures were obtained by averaging the 24 individual temperatures. Note missing data due to flooding event Fall 2014. The bold red line approximately reflects upper lethal limits for fry (24.7oC) and juvenile (23.4oC) Rio Grande cutthroat trout (Zeigler et al. 2013). Figure 3. South McKittrick (Reach 3) weekly average water temperature (7d WAT). Note missing data due to flooding event Fall 2014. The bold red line approximately reflects upper lethal limits for fry (24.7oC) and juvenile (23.4oC) Rio Grande cutthroat trout (Zeigler et al. 2013). Table 1. Water temperature (oC) summary for South McKittrick (Reach 3). Year Total number of days logged 2015 247 Maximum Minimum hourly hourly 29.5 0.0 Maximum Average 24 h Minimum Average 24 h 7d WAT Maximum 7d WAT Minimum 25.1 1.5 24.2 2.4 Table 2. Population estimate (fish/100 m2) for rainbow trout conducted in the main stem (Reach 1) and South McKittrick Creek (Reach 2-4). 95% confidence intervals presented in parenthesis. * indicates that fish were not captured after the first fish collection pass and thus population estimates were not estimated within these upper reaches. Site Fall 2013 Spring 2014 Fall 2014 Reach 1 5.5 (5.2-5.7) 28.1 (27.0-29.2) 11.3 (11.2-11.5) Reach 2 7.7 (7.6-7.8) 17.4 (15.4-19.3) 8.8 (8.6-9.0) Reach 3 0.6* 0.2* 3.6* Reach 4 0.9* 0.4* 4.8 (4.3-5.2) 100 Fall 2013 (n = 42) Spring 2014 (n = 144) Fall 2014 (n = 99) Frequency (%) 80 60 40 20 0 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Total length (cm) Figure 4. Length frequency (%) of rainbow trout captured and combined across the four study reaches.