susceptibility of antibiotic-resistant bacterial strains to

K. De Smet, D. Van den Plas, D. Lens and P. Sollie
Flen Pharma NV, Research Department, Edegem (Antwerp), Belgium.
Aim: A new, optimized, antimicrobial enzyme system was developed for the control of wound
bioburden. This Glucose oxidase – Lactoperoxidase – Guaiacol (GLG) enzyme system, incorporated in
an alginate gel dressing, was analyzed for antimicrobial activity against antibiotic-resistant bacterial
strains and for cytotoxicity towards keratinocytes and fibroblasts.
Method: The susceptibility of a wide range of antibiotic-resistant bacterial strains (clinical isolates) to
the GLG enzyme system was analysed using Minimal Inhibitory Concentration (MIC90) determination.
Further, challenge tests and cytotoxicity tests were performed with Flaminal ® Forte, a new hydroactive
alginate gel dressing with antimicrobial activity, obtained by the presence of 1.5% GLG enzyme
Results: The MIC90- concentrations for most of the tested strains were in the range of 0.015% – 0.06%
GLG enzyme system and for all Methicillin-Resistant Staphylococcus aureus (MRSA) strains 0.008%.
Challenge tests show that for 11 strains all the bacterial cells are killed within 3 hours of incubation.
For the other 3 strains all the bacterial cells are killed within 6 hours of incubation. Cytotoxicity
determined by measuring metabolic activity using MTT assay on cells incubated in a 20% Flaminal®
Forte solution showed no decrease in cell survival compared to the control cells. In contrast, other
antimicrobial wound care products showed a high degree of cytotoxicity.
Conclusion: With the increasing concern of bacterial resistance towards antibiotics, this study clearly
shows that low concentrations of the GLG enzyme system are successful in killing antibiotic-resistant
bacterial strains. Furthermore, results show that Flaminal® Forte combines strong antimicrobial
activity with non-cytotoxicity, promoting optimal wound healing.
Related flashcards

25 Cards

Alcohol abuse

33 Cards

Agrarian parties

61 Cards

Food preservation

22 Cards

Create flashcards