DANAGENE Genomic DNA Kit
Kit designed for an efficient and fast
purification of highly pure genomic DNA from a
wide variety of samples including: cultured cells,
animal tissues, mouse tail, paraffin embedded
tissues, Gram ( -) b a c t e r i a , G r a m ( + )
b a c t e r i a , y e a s t , Drosophila melanogaster,
body fluids (saliva, semen, plasma, urine,
serum, etc...), hair, blood stain.
Procedure: The process includes a cell lysis with an
anionic detergent that solubilizes the necessary cell
components, the contaminant RNA can be removed
with a RNase treatment. The cell proteins are
removed by precipitation, which allows to leave the
genomic DNA in solution. Finally, the genomic
DNA is isolated by a precipitation with isopropanol.
Features:

Reproducible, fast and non-expensive
method.

Convenient and scalable purification
procedure.

Safe method, as it removes completely the
need of using toxic reagents.

Allows to process different biological samples.

A high quality DNA is obtained, with
an A260/280 of 1.7 - 1.9 ratio.
Cell lysis
M
Protein DNA
Precipitation
1
2
3
4
DNA Wash
5
DNA
Hydration
6
Applications: DNA purified using this kit is
highly stable and suited for use in a wide range of
applications such as:





DNA archiving.
PCR and quantitative real-time PCR.
SNP analysis.
Southern Blotting.
Sequencing.
DANAGENE Genomic DNA Kit.
Ref.0603.1.RNase and Proteinase K included.
For 50 extractions from 20 mg of mouse tissue and
mouse tail / 50 extractions from 4 x 106 culture
cells / 50 ml bacteria culture / 100 ml yeast culture.
DANAGENE Genomic DNA Kit.
Ref.0603.2.RNase and Proteinase K included.
For 200 extractions from 20 mg of mouse tissue
and mouse tail / 200 extractions from 4 x 106
culture cells / 200 ml bacteria culture / 400 ml
yeast culture.
Genomic DNA analysis in agarose gel.
Prepared with DANAGENE Genomic DNA Kit.
M: REAL Escale N.8
1. Mouse Brain.
4. Mouse Kidney.
2. Mouse Intestine. 5. Mouse Heart.
3. Mouse Liver.
6. Mouse Lung.
DANAGENE Genomic DNA Kit.
Ref.0603.3
For 200 extractions from 20 mg of mouse
tissue and mouse tail / 200 extractions from
4 x 106 culture cells / 200 ml bacteria culture /
400 ml yeast culture