Supplementary materials 1. Analytical Methods TS and VS of substrates and inoculum were measured in triplicate per sample according to standard methods [1]. Organic elements (CHNS) were analyzed by an elemental analyzer (Vario EL cube, Germany). Pressures in the head of digesters were measured by a 3150 WAL-BMP-Test system pressure gauge (WAL Mess-und Regelsysteme GmbH, Germany). The volume of biogas produced was calculated according to ideal gas law [2]. Biogas composition was measured by a 6890A gas chromatography (Agilent Company, USA) equipped with a thermal conductivity detector (TCD) and Argon was the carrier biogas at a flow rate of 30 mL/min. A 1.8 m × 80/100 stainless steel column (All-tech, USA) was used. The temperatures of the oven, injector port, and detector were 100, 120, and 120 °C, respectively. Biogas composition was measured every day in the first week, then once or twice per week until the end of digestion. Measured methane content was linearly interpolated to determine the daily methane content [2]. 2. Statistical Analysis The significance of biogas and methane production were obtained by using analysis of variance (one way ANOVA) at α = 0.01 and α = 0.05. Origin 8.0 (OriginLab, USA) and PASW Statistics 18.0 (IBM, USA) were used for data and graphing analysis. 3. Floating separation tests Several batch tests were conducted for floating separation. The first one was operated at room temperature, with MS-OFMSW to water ratio of 1:9, 3:7, and 5:5 (on weight basis), respectively, and initial mixing. After 30 h floating, different layers were appeared. Three layers were shown to inert (fixed) materials, water solution, and organics (machine- and floating-separated OFMSW, MFS-OFMSW) from the bottom up. As seen in Table S1, with the percentage of MS-OFMSW in water increasing, the VS/TS ratio of MS-OFMSW increased. Highest VS/TS ratio of 75.2% was found with MS-OFMSW to water ratio of 1:9. Thus, MS-OFMSW to water ratio of 1:9 was used for further tests. Then, we found that increasing the temperature could speed up the generation of floating and sunken layers. In the current study, MFS-OFMSW was obtained after settling for 2 h with temperature maintain at 50 oC. A 1 L glass cylinder was used and the MS-OFMSW to water ratio was 1:9 (on weight basis). The VS/TS ratio for MFS-OFMSW was 81.2% (Table 1). References: 1. Li, Y.Q., Feng, L., Zhang, R.H., He, Y.F., Liu, X.Y., Xiao, X., Ma, X.X., Chen, C., Liu, G.Q.: Influence of inoculum source and pre-incubation on bio-methane potential of chicken manure and corn stover. Appl. Biochem. Biotechnol. 171, 117-127 (2013) 2. El-Mashad H.M.: Kinetics of methane production from the codigestion of switchgrass and Spirulinaplatensis algae. Bioresour. Technol. 132, 305-312 (2013) Table S1 Characteristics of MS-OFMSW and MFS-OFMSW in previous study Samples TS, % VS (%TS) MS-OFMSW 23.5 54.0 MFS-OFMSW(1:9)* 8.3 75.2 MFS-OFMSW(3:7)* 19.9 60.1 MFS-OFMSW(5:5)* 23.6 52.3 *: MS-OFMSW to water ratio (on weight basis) Fig. S1. Floating separation test for MS-OFMSW at ambient temperature