Supplemental Table 1. RNA and protein expression levels of SLC22A1 (OCT1), SLC22A3 (OCT3), SLC6A4 (SERT) and AOC1 (DAO).
Expression levels of the RNA and the protein localization data were extracted from the human tissue samples available in the Human
Protein Atlas (http://www.proteinatlas.org/, accessed on July 25, 2015) [1,2]. The RNA expression levels were obtained from RNA
sequencing and the values are expressed as FPKM (Fragments Per Kilobase of transcript per Million mapped reads). The higher the
FPKM value, the higher the expression levels. The expression levels of the genes in four different sections of the intestinal tissues that are
available in Human Protein Atlas were obtained and entered in this table. Expression levels of the genes in kidney and liver were entered
for comparison purposes. In general, publications arbitrarily define expression threshold as FPKM > 0 to identify expressed transcript.
Protein expression levels were determined using antibody staining and the intensity were entered in the table below as annotated in the
Human Protein Atlas. The different cell types of each tissues are explained in this site http://www.proteinatlas.org/learn/dictionary.
RNA Expression (FPKM)
Protein Expression (Immunohistochemistry)
SLC22A1
(OCT1)
SLC22A3
(OCT3)
SLC6A4
(SERT)
*AOC1
(DAO)
Tissues
SLC22A1
(OCT1)
SLC22A3
(OCT3)
0
3
2
18
Stomach
Glandular
Cells: Low
and Medium
Glandular
Cells:
Medium
0
2
11
130
Duodenum
Glandular
Cells:
Medium
Glandular
Cells:
Medium
0
0
3
3
44
1
161
120
Small
Intestine
Glandular
Cells:
Medium
Glandular
Cells:
Medium
Colon
Endothelial
Cells:
Medium;
Glandular
Cells:
Medium;
Peripheral
nerve/ganglio
n: Medium
Endothelial
Cells:
Medium;
Glandular
Cells:
Medium;
Peripheral
nerve/ganglio
n: Medium
SLC6A4
(SERT)
Not
Available
in Human
Protein
Atlas.
Han TK et
al. (2015)
[3] showed
that SERT
protein
levels in
the
jejunum is
expressed
at similar
levels with
OCT1.
*AOC1 (DAO)
Glandular
Cells: Low
Glandular
Cells: Medium
Glandular
Cells: Medium
Endothelial
Cells: Low;
Glandular
Cells: High;
Peripheral
nerve/ganglion
: Low
2
315
12
65
1
0
159
2
Kidney
Liver
Cells in
glomeruli:
Medium;
Cells in
tubules:
Medium
Bile duct: Not
Detected
Hepatocytes:
Medium
Cells in
glomeruli:
Medium;
Cells in
tubules: High
Cells in
glomeruli:
Low; Cells in
tubules: High
Bile duct:
Medium;
Hepatocytes:
Medium
Bile duct: Low
Hepatocytes:
Not Detected
*Previous symbol for AOC1 gene is ABP1
References
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doi:10.1126/science.1260419
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