DNase treatment for RNA
*Preheat block to 37C
1. Prepare 1.5 ml tube with the following:
a. 1000ng RNA
total volume: 17 µl
b. DEPC water
c. 2 µl DNase I buffer
d. 1 µl DNase I enzyme
2. Pipet to mix, spin down.
3. Incubate at 37C/4hrs
4. Keep Dnase treated RNA on ice
5. PCR: check that there is no DNA.
6. Inactivate: Add 4 µl EDTA (25mM), and incubate at 75C/10 min.
7. Store RNA at -20C.