DNase treatment for RNA *Preheat block to 37C 1. Prepare 1.5 ml tube with the following: a. 1000ng RNA total volume: 17 µl b. DEPC water c. 2 µl DNase I buffer d. 1 µl DNase I enzyme 2. Pipet to mix, spin down. 3. Incubate at 37C/4hrs 4. Keep Dnase treated RNA on ice 5. PCR: check that there is no DNA. 6. Inactivate: Add 4 µl EDTA (25mM), and incubate at 75C/10 min. 7. Store RNA at -20C.