FAQs: Anaerobic, Aerobic, Anoxic BACKGROUND In wastewater treatment cells grow until the reach maximum size, and then divide into two in a process called binary fission. This process can happen every 20 to 30 minutes under optimum growth conditions. During cell growth, there are maintenance needs that the bacteria have. These maintenance needs are met through energy released from food molecules. All bacteria have enzymes, which enable them to break down food and pass it through the cell membrane. Respiration: The process of obtaining energy from food molecules which is controlled by enzymes. Enzymes: Protein molecules that each cell makes. Enzymes are needed for all biochemical reactions because they help the cell break down the organic materials so they can go through the cell membrane (outer wall of the cell). Synthesis: An energy consuming reaction that results in the production of new biomass Endogenous Respiration: When synthesized biomass and storage products become the energy source for the reaction. This decreases the amount of biomass present. AEROBIC RESPIRATION This is how BOD is removed in activated sludge. Oxygen is the final electron receptor in these reactions. Sources of energy (food) include carbohydrates, and proteins. End products of aerobic respiration include water, energy, and carbon dioxide. Aerobic: Free dissolved oxygen is present Aeration Tank Aerobic Organisms: These organisms only have enzyme structures capable of functioning in aerobic environments ANAEROBIC RESPIRATION Anaerobic respiration occurs in the absence of free dissolved oxygen. Oxygen is still required for respiration, but is derived from nitrate (NO3-), Sulfate (S04-2) and Carbonate (CO3-2) Bound Oxygen Molecule Nitrate Sulfate Carbonate End Product Nitrogen Gas Hydrogen Sulfide Methane WHAT’S THE DIFFERENCE BETWEEN ANOXIC AND ANAEROBIC? Anaerobic: A condition in which free of combined oxygen is not available Anoxic: A condition in which oxygen is available in combined forms only o While technically anaerobic, reactions that involve using inorganic electron acceptors (nitrate and sulfate) are often referred to as anoxic TYPES OF ORGANISMS Aerobic Organisms: Use aerobic respiration only Anaerobic organisms: Use anaerobic respiration only Facultative Organisms: Can use both aerobic and anaerobic respiration. They will use aerobic respiration in the presence of free dissolved oxygen. REFERENCES Activated Sludge Processes, METC Group Incorporated, 1999 Operation of Municipal Wastewater Treatment Plants, Volume II: Liquid Processes Sixth Edition, WEF