Institut für Analytische Chemie
Währinger Straße 38
A-1090 Wien
T +43-1-4277-52301
F +43-1-4277-9523
Sehr geehrte Damen und Herren,
das Institut für Analytische Chemie lädt zum Vortrag von
Prof. Pier-Giorgio Righetti
Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”,
Politecnico di Milano
zum Thema
“The Monkey King and Pigsy ferrying the proteomic sutras into the third
millennium”
Termin:
Ort:
Donnerstag, 02. Oktober 2014, 14:30
Hörsaal II, Währinger Straße 42, 1090 Wien
Abstract:
About fifty years of progress in “proteome” analysis, starting from primitive two-dimensional (2D)
maps attempts in the early sixties of last century, will be here covered. The polar star in 2D
mapping arose in 1975 with the classical paper by O’Farrell in J Biol. Chem. It became the
compass for all proteome navigators. Perfection only came, though, with the introduction of
immobilized pH gradients, which fixed the polypeptides spots in the 2D plane. Great impulse in
proteome analysis came by introducing informatics tools and creating databases, among which
Swiss Prot remains the site of excellence. Towards the end of the nineties, 2D chromatography, as
epitomized by coupling strong cation exchangers with C18 resins, began to be a serious challenge
to electrophoretic 2D mapping, although up to the present both techniques are still much in vogue
and appear to give complementary results. Yet the migration of “proteomics” into the third
millennium was only made possible by mass spectrometry (MS, the Monkey King!), which today
represents the standard analytical tool in any lab dealing with proteomic analysis. Another major
improvement has been the introduction of combinatorial peptide ligand libraries (CPLL, Pigsy!),
which, when properly used, enhance the visibility of low-abundance species by 3 to 4 orders of
magnitude. Coupling the Monkey King with Pigsy permits to explore at least 8 orders of magnitude
in dynamic range on any proteome.