FACULTY OF BIOTECHNOLOGY AND ANIMAL HUSBANDRY
LIST OF COURSES
ACADEMIC YEAR 2010/2011
Course title:
BASICS OF GENETICS
Name of the lecturer:
Daniel Polasik, PhD
ECTS points:
5
Language of instruction:
English
Semester:
SUMMER
Hours per week:
1
Code:
1-WBiHZ-10/11
Teaching method:
lecturers
Entry requirements:
Basics of molecular biology and biochemistry
Objectives of the course:
Get knowledge about inheritance of traits and modern techniques in molecular genetics
Course contents:
History of genetics. Terms using in genetics. Inheritance of quantitative and qualitative
traits. Structure of DNA and genetic code. Mutations and others reasons of biodiversity.
Genes expression and their regulations. Genetic and physical genome maps. Population
genetics. Methods based on PCR.
Assessment method:
Credit of the lecturers (test)
Recommended readings:
E. Passarge "Color Atlas of Genetics", Thieme Medical Publishers, 2007.
B. Lewin "Genes VII", Oxford University Press, 2000.
P.C. Winter, G.I. Hickey, H.L. Fletcher, "Instant Notes in Genetics" Bios Scientific Publishers
Limited, 2003.
Course title:
BIOTECHNOLOGY AND GENETIC ENGINEERING
Name of the lecturer:
Arkadiusz Terman, PhD
ECTS points:
5
Language of instruction:
English
Semester:
WINTER
Hours per week:
1
Code:
2-WBiHZ-10/11
Teaching method:
laboratory
Entry requirements:
Molecular biology, genetics
Objectives of the course:
The course discusses current knowledge about the genetic engineering in biotechnology
and molecular mechanisms.
The course include: genomic DNA isolation, RNA isolation, primers design, PCR analysis,
use of RFLP, AFLP, RAPD and RT-PCR methods, restriction enzymes analysis, agarose gel
electrophoresis, gene expression analysis, practical use of different software for genomes
analysis
Course contents:
Assessment method:
Exam
Recommended readings:
B. Lewin "Genes VII", Oxford University Press, 2000.
T. A. Brown "Genomes - Third Edition", Garland Publishing, 2006.
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FACULTY OF BIOTECHNOLOGY AND ANIMAL HUSBANDRY
Course title:
ELEMENTS OF PARASITOLOGY
Name of the lecturer:
Agnieszka Tomza-Marciniak, PhD
ECTS points:
4
Language of instruction:
English
Semester:
WINTER
Hours per week:
10h/semester
Code:
3-WBiHZ-10/11
Teaching method:
lecture
Entry requirements:
Objectives of the course:
Protozoology, inverbrate zoology
The course aims to provide students with knowledge of the biology, physiology, morphology
of the major parasites of humans and domestic animals and diagnostic methods using in
parasitology.
Course contents:
Parasitism as biological phenomena. Terminology. Parasites occurrence. Morphological
adaptation to parasitism. Immunological aspects of host parasite relationship. Type of
immune response. Host immunity against parasites. Evasion of host immunity by parasites.
Biological properties of host-parasites relationship. Reproduction, development and life
cycles. Adaptation to closing of the life cycle. Physiology of host-parasite relationship. The
course of parasite penetration. The pathological changes caused by endoparasites in host.
Diagnostic parasitology – classical (coproscopy and larvascopy methods), molecular
methods – ELISA test, PCR, electrophoresis).
Assessment method:
test results
Recommended readings:
Roberts L.S., Janovy J., 2009: Foundations of Parasitology. McGraw-Hill.
Robinson E., 2006. Diagnostic Parasitology for Veterinary Technicians. Elsevier.
Course title:
FUNDAMENTALS OF LABOLATORY DIAGNOSTICS
Name of the lecturer:
Agnieszka Tomza-Marciniak, PhD
ECTS points:
5
Language of instruction:
English
Semester:
WINTER
Hours per week:
1
Code:
4-WBiHZ-10/11
Teaching method:
laboratory practice
Entry requirements:
Objectives of the course:
Course contents:
Physiology, animal anatomy
The course aims to provide students with knowledge of laboratory tests check a sample of
blood, urine, faeces and body tissues.
Complete Blood Count (CBC) test. Automated and semi-automated blood analysis. The
urine analysis (Reader Urine Analyser). The physico-chemical and microscopic properties
of the urine. Urine sample collection and analysis. Urine sediment analysis. The qualitative
and quantitative methods in parasitology. Coproscopic techniques for detection and
quantitative estimation of endoparasites. Microscopic Examination. The post-mortem
parasitological examination: dissection, parasites isolation, preservation and examination
of collected samples. Cryptosporidium detection methods. Zielh-Nielsen staining method:
preparation and microscopic examination. Cryptosporidium immunochromatographic
test. Trichinella detection: trichinoscopy and pooled-sample digestion method.
Assessment method:
Practice exam
Recommended readings:
Pagana K., Pagana T., 2006: Mosby's Diagnostic and Laboratory Test Reference. Elsevier
Health Sciences.
Garcia L., 2009: Practical Guide to Diagnostic Parasitology. American Society for
Microbiology.
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Course title:
FACULTY OF BIOTECHNOLOGY AND ANIMAL HUSBANDRY
PROTEOMICS
Name of the lecturer:
Małgorzata Ożgo – Asist. prof.
Katarzyna Michałek - PhD (Eng.)
Alicja Dratwa – PhD (Eng.)
Agnieszka Herosimczyk - MSc (Eng.)
Adam Lepczyński - MSc (Eng.)
ECTS points:
5
Language of instruction:
English
Semester:
SUMMER
Hours per week:
1
Code:
5-WBiHZ-10/11
Teaching method:
Lectures and laboratory exercises
Entry requirements:
Biology, chemistry, biochemistry, genetics,
Objectives of the course:
Course contents:
The purpose of teaching is to learn and understand:
 biological significance of proteome organization
 significance of protein identification
 significance of identification differences between protein profiles
 selected proteomic techniques
 actual trends in proteomic researches
Introduction to proteomics. Genome, transcriptome, proteome. Biological significance of
post-transcriptional and post-translational protein modifications. Proteome organization.
Proteome analysis and protein identification. Sample preparation. The purpose of
proteome analysis and protein identification, cellular lysis, lysis buffers (chaotropic
agents, detergents, reducing agents, amfolites), methods of sample purification, methods
of protein precipitation. Protein separation techniques. The general principles of
proteomic analysis. Basic components of polyacrylamide gels, proteins detection,
methods of staining proteins. One-dimensional gel electrophoresis, stacking gels,
resolving gels, SDS-PAGE electrophoresis, native PAGE electrophoresis, technique of
preparation and usage of mini gels. Electrophoresis – 2D. Rehydration significance,
principles of isoelectric focusing, isoelectric point, rehydratation buffers, IPG balancing
strips, migration buffers, second- dimension of 2D-SDS PAGE electrophoresis. Western
blotting. Transfer of proteins from gel to membrane. Wet transfer, semi-dry transfer.
Immunodetection. Mass Spectrometry (MS) application in proteomics. Maldi-ToF mass
spectrometry - Matrix-Assisted Laser Desorption/Ionization (MALDI), Time-of-Flight (TOF)
mass spectrometry, proteolytic enzymes usage in sample preparation. Bioinformatic
tools. Bioinformatic programs: Quantity One, PDQuest, BioTool. Usage of this programs for
proteome analysis. Quantity and quality proteome analysis. Bioinformatic databases,
protein identification principles, Peptide Map Fingerprinting –PMF. Proteomics in Poland
and in the World. Current progress in proteomic studies in the world and their practical
application. The purpose of proteomics. HUPO – Human Proteome Organization.
Assessment method:
To receive a credit for a course in proteomics, students have to get positive mark in a
test.
Recommended readings:
Walker J.M. (Ed.). The Proteomics Protocols Handbook. Softcover. 2005.
Liebler D.C. Introduction to proteomics. Tools for the new biology. Humana Press. 2002.
Palzkill T. Proteomics. Kluwer Academic Publishers. 2000.
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