STAAR/EOC Academy Johnson/McCollom Types of Cells Eukaryotic-You and Me - Animals and Plant Prokaryotic-Bacteria- PRO means NOOOO Nucleus -Eubacteria and Archaebacteria Cell Organelles A. Cell Wall-Protection and Support-In plant cells and Bacteria as well. B. Nucleus-Control Center of Cell-The Brain C. Ribosomes-Makes Protein-Translations occurs here-Found on Rough ER-Small Dots D. Endoplasmic Reticulum-Smooth and Rough-Assembles Proteins-Rough has Ribosomes -Looks like a maze -Located near Nucleus E. Mitochondria-Power Plant of the Cell- Cellular Respiration occurs here- Makes ATP. -C6 H12 O6 + O2 CO2 + H20 + ATP- Looks like a Snake F. Lysosome-Digestion occurs here-Stomach of the Cell G. Centriole-Cell Division- Small dots located near Nucleus-Spindle fibers attach here. H. Chloroplast-Found in Plants ONLY. Photosynthesis occurs here-Looks like Poker Chips -CO2 +H2O O2 + H2O + C6 H12 O6 I. Cell Membrane-Protection and Support-Phospholipid Bilayer-Made of Phospholipids, Fatty acid tails and Protein Channels-Selectively Permeable- Allows things in and out of the cell J. Nucleolus-Small dark dot in the center of the Nucleus-Makes Ribosomes K. Chromosomes-Make DNA-Found in the Nucleus-Blue Print of life. L. Golgi Body/Golgi Apparatus-Packages proteins-Looks like pancakes-UPS/FedEx M. Vacuole-Water and mineral storage-Large in plants. N. Cytoplasm-Liquid portion of the cell-Water in the pool. O. Flagella-long whip-like structure found on some cells. Used for locomotion/movement - Sperm P. Cilia-hair-like structures found on some cells-Used for locomotion/movement Q. Nuclear Membrane/Envelope-Membrane around the nucleus-Allows things in and out. Homeostasis-Process by which organisms maintain a relatively stable internal environment. Constant internal condition. Regulates what is in and out of the cell. Cell membrane-Also called Plasma Membrane or Phospholipid Bilayer. Regulates what enters and leaves the cell. Also protects and supports it. Made of phospholipids. Phospholipid Cell Membrane Membrane Functions Diffusion-Where particles pass through a membrane fro a high to low concentration gradient. No energy required. Osmosis-A type of diffusion that is only associated with WATER. High to low concentration gradient. No energy required A. Isotonic-Water particles are balanced on the inside of the cell as well as the outside. B. Hypertonic-There is more water in the cell that outside of the cell so the cell shrinks. EX. 90% in the cell and 10% outside. Remember water in the body will balance out. C. Hypotonic-There is more water on the outside of the cell than inside. So the cell will swell and possibly burst. Hypo sounds like HIPPO. Hippos are FAT!!!!! EX. 10% water in cell and 90% water outside. Remember that water will balance out. Johnson/McCollom Facilitated Diffusion-Type of diffusion where large molecule cannot diffuse across the phospholipids. Molecules have to pass through the protein channels. High to low concentration. DOES NOT REQUIRE ENERGY!!!!!! Active Transport-DIFFERENT than the other types of diffusion. LOW to HIGH CONCENTRATION. This process requires the cell to transport material in the opposite direction. Usually requires the protein channels. PROCESS REQUIRES ENERGY!!!!! Very important to know this!!!! Diffusion/Osmosis Facilitated Diffusion Active Transport Other information about cells Pinocytosis- Process by which a cell takes in liquid from the surrounding environment- CELL DRINKING Phagocytosis- Process by which extensions of cytoplasm surround and engulf large particles and take them into the cell. CELL EATING Endocytosis- Process by which a cell takes material into the cell by folding of the cell membrane. Exocytosis- Process by which a cell releases large amounts of material. EXO sounds like exit. Go to the bathroom! Adenosine Triphosphate (ATP) - one of the principal chemical compounds that living things use to store and release energy. Made in the MITOCHONDRIA by CELLULAR RESPIRATION. ENERGY!!!!!!! Adenosine Diphosphate (ADP)-reduced when an ATP molecule looses one of its phosphate molecules Photosynthesis (Plants or Autotrophs) and Cellular Respiration (Animals or Heterotrophs) Johnson/McCollom STAAR/EOC-Cells, Organelles, and Functions Animal Cell Plant Cell Johnson/McCollom Specialized Cells Many organisms are multi-cellular - they are made up of lots of cells, not just one! Many of these cells are specialized, sharing out the life processes (they work together as a team, supporting the organism). Specialist cells occur in both animals and plants… Animal/ Plant Cell Picture Function Specialization To carry oxygen Large surface area for oxygen Hemoglobin which binds the oxygen No nucleus To carry nerve impulses to different parts of the body Long Connections at each end Can carry electrical signals To store fat when there is excess To absorb water and minerals Large surface area which helps it to absorb water and minerals Thin cell wall makes it easy for minerals to pass through To absorb sunlight (needed for photosynthesis) Large surface area Many chloroplasts (containing chlorophyll, for photosynthesis) To conduct water, minerals, and nutrients through out a plant Elongated, tubular shape with thin walled sieve tubes Consists of xylem and phloem vessels Gives mechanical strength to the plant Red blood cells Nerve cell Large Round Empty looking Fat cells Root hair cells Leaf cell Stem cells Unit 2 Biomolecules!!!! You are what you eat!!! Swain/Gaas Carbohydrates-You get these from all plant matter! Carbohydrates are made of CARBON, HYDROGEN, and OXYGEN. Monomers (BUILDING BLOCKS) are sugars. Also called monosaccharides or glucose. C6 H12 O6. Functions (THEIR JOB) is to supply the body with ENERGY!! Also in plants they are for structure and support (CELL WALL) Examples are Glucose-Blood Sugar STARCH-Complex sugar found in Potatoes, Corn, and Pasta. NOT found in MEAT!!! This is energy!!!! Cellulose-Used to make cell walls in plants, Support and Structure, Fiber for humans. We cannot digest it!!! Polysaccharides (Polymers) are long chains of monosaccharides. Like a chain. Very Complex Sugar!!! Chemical Indicators- Benedicts Solution tests for Sugar!!! Will turn sugars ORANGE!! Lugol’s or Iodine will turn STARCH black. Molecular Structure Monosaccharide Bread Polysaccharides are Complex. EX STARCH Pasta Fruit and Vegetables Potatoes=Starch ALL CARBOHYDRATES ARE FOR ENERGY!!!!!!!!!!!!!! Unit 2 Biomolecules!!!! You are what you eat!!! Swain/Gaas Lipids- You get these from Animal Fats, Oils, Nuts, and BEESWAX!!! Lipids are made of CARBON, HYDROGEN, AND OXYGEN. Monomers (BUILDING BLOCKS) are or is 1 glycerol and 3 fatty acid tails. Functions of lipids are to STORE ENERGY. Ex BODY FAT. Also MAKES up part of the cell membrane. Lipids function also as an insulator in the winter. Ex Coat or Blanket. Reduces heat loss. Examples are Body Fat-Storage Oils and Waxes!! Cooking Oil, Beeswax, Car Wax, Candle Wax!!!! Cell Membrane- Phospholipid Bilayer or Plasma Membrane. Hormones-Testosterone and Estrogen. Chemical Indicators- None. Identify lipids by placing a drop of a substance on a piece of paper. If it becomes translucent it is a lipid. Ex. French Fries from McDonalds that have been sitting in a bag for a while. Oil or Grease will make the bag somewhat see through. Saturated Fat=Solid at room temperature. Unsaturated=Liquid at room temperature. Molecular Structure Saturated and Unsaturated Fats Meat(Saturated) Glycerol and 3 Fatty Acid Tails Fish Oils(Unsaturated) Beeswax All LIPIDS ARE FOR STORAGE!!!!!!!! Unit 2 Biomolecules!!!! You are what you eat!!! Swain/Gaas Proteins- You get these from All Animal Products and Beans. Does not come from POTATOES (Starch)!!!!! Proteins are made of CARBON, HYDROGEN, OXYGEN, NITROGEN, and SULFER. Monomers (Building Blocks) are AMINO ACIDS. Function of proteins are to BUILD MUSCLE, MAKES ENZYMES, and STRUCTURE AND SUPPORT OF ALL ORGANISMS. ENZYMES SPEED UP CHEMICAL REACTIONS. Examples are Muscles- If you want big muscles eat a lot of protein and lift weights!! Hair and Cartilage- Protein Rich Shampoo and Lotion!!! Hormones- Testosterone and Estrogen. Polypeptides (Polymers) are long chains of amino acids. They are together by peptide bonds. Chemical Indicator- Biuret Solution tests for protein. Protein will turn light purple if it is present in the food source. Molecular Structure Amino Acid Farm Animals (Animal Products) Polypeptides Beans Polypeptides Tofu ALL PROTEINS ARE FOR STUCTURE AND SUPPORT (MUSCLE GROWTH). THEY ALSO MAKE ENZYMES AND HORMONES!!!! Unit 2 Biomolecules!!!! You are what you eat!!! Swain/Gaas Nucleic Acids- You do not eat these!!! DNA and RNA you get this from your parents! Monomers (Building Blocks) are Nucleotides. They are made of a PHOSPHATE, 5 CARBON SUGAR, and a NITROGEN BASE. Phosphate and the 5 carbon sugar make the backbone of DNA and RNA. Functions (THEIR JOB) are to store genetic material (DNA-Blue Print of Life) and to make proteins (RNA-Protein Synthesis). Synthesis means to create or make!!! Examples are Deoxyribonucleic Acid (DNA-Double Stranded) and Ribonucleic Acid (RNASingle Stranded) Molecular Structure Nucleotide DNA-Blueprint (instructions) Nucleotide RNA-Makes Proteins DNA IS THE BLUEPRINT OF LIFE AND RNA MAKES PROTEINS! TEKS 6C - Protein Synthesis (Transcription and Translation) Recall that the transcription process is DNA to RNA and translation is RNA to Protein. Each strand represents a DNA sequence. Each strand will start with a heart bead. Color code for beads • Cytosine = red • Adenine = white • Thymine = blue • Guanine = yellow • Heart or Star Bead= no meaning 1. Record the DNA base sequence of the strand that starts with a heart bead here: DNA____________________________________________________________________ o This should be a sequence of letters, G for guanine, T for Thymine and so on. 2. Next, create an mRNA strand using the DNA sequence from above. Write it here: mRNA___________________________________________________________________ Remember that Uracil (U) will replace Thymine (T). o The process you have just modeled is called ________________________ 3. Go back to step 2 and put a line after every third letter. o Each group of 3 nucleotides is called a ____________. 4. Use the mRNA codon chart (below) to determine the amino acids that tRNA will bring to each codon. o ____________________________________________________________________ o The process you have just modeled is called ________________________. http://www.edhsgreensea.net/Biology/taters/CST.htm Johnson/Hill Protein Synthesis DNA Contains double strand of nucleotides made of deoxyribose sugar, phosphate, and nitrogen bases (Guanine, Thymine, Adenine, Cytosine) provides instructions for the production of proteins in a process known as protein synthesis too large to leave the nucleus, so the cell needs to make RNA to send the protein making instructions to the ribosomes RNA contains single strand of nucleotides made of ribose sugar, phosphate, nitrogen bases (guanine, adenine, cytosine, Uracil) 3 types of RNA: o Messenger (mRNA)- transcribes (copies) the DNA instructions to take to the cytoplasm o Transfer (tRNA)- uses anticodons to read sections of mRNA so amino acids can attach to form a protein o Ribosomal (rRNA)- structural parts of ribosomes that help to build proteins Base Pairing Rules: DNA RNA A–T A–U C–G C–G Steps of protein synthesis: Transcription- mRNA is made from DNA template and the mRNA single strand is sent to cytoplasm to find a ribosome. This begins the protein making process. Translation- tRNA brings amino acids to the mRNA codon sections to be “read” to create the polypeptide strand http://commons.wikimedia.org/wiki/File:Mrna.gif Johnson/Hill Characteristics Archaebacteria Eubacteria Protista Fungi Plantae Animalia Type of Reproduction asexual asexual asexual / sexual asexual / sexual asexual / sexual asexual / sexual Method of Movement flagella flagella cilia, flagella, pseudopods immobile immobile mobile (various) Examples methanogens Prokaryotic or Eukaryotic Has a nucleus? Single-celled or Multicellular Method for Obtaining Energy Word Bank (Use each term/group of terms once): prokaryotic eukaryotic eukaryotic eukaryotic eukaryotic prokaryotic autotrophs immobile heterotrophs single-celled multicellular immobile multicellular single-celled flagella flagella asexual asexual chemotrophs yes yes no yes no yes mobile (various) asexual/sexual asexual/sexual asexual/sexual asexual/sexual methanogens both (mostly single-celled) lions/birds/sponge/jellyfish cyanobacteria/streptococcus ferns/moss/flowers both (mainly multicellular) mushrooms/yeast/lichen amoeba/paramecium/algae cilia, flagella, pseudopods autotrophs/heterotrophs/chemotrophs Heterotrophs (protozoa)/autotrophs (algae) Goodland/Bishop Terms to be familiar with when distinguishing between the 6 Kingdoms of Life. Prokaryote – A cell that does not contain a true nucleus. The cell contains DNA; however, the DNA is not encapsulated within a nucleus. Simple cell, less complex, does not have membrane-bound organelles. Eukaryote – A cell that contains a true nucleus. The cell contains DNA within a nucleus. Larger cell, more complex, has membrane-bound organelles. Heterotroph – used to explain an organism that consumes its energy. These organisms cannot make their own food and do not have organelles to do so. Autotroph – An organism that synthesizes its energy within the cell itself. The organism can do this by using sunlight or chemicals. Both are considered inorganic. Examples: Photoautotroph or Chemoautotroph Cell Wall – An extra layer that surrounds the Cell Membrane. Can be composed of Cellulose (a carbohydrate found in plant cell walls) or Chitin (a carbohydrate found in Fungal Cell Walls). Bacterial Cell Walls can also have different layers as well. Flagella – a long whip-like tail attached to a cell that is used for mobility. Cilia – many tiny little hairs that surround the cell and are also used for mobility. Goodland/Bishop CELLULAR RESPIRATION AND PHOTOSYNTHESIS- HANDOUT *Energy, ATP and Cellular Respiration: Energy is the ability to do work (muscle contraction). Energy can change from one form to another, but is not created or destroyed. Chemical energy stored in ATP can be converted to muscle contraction. Our bodies constantly use chemical energy for necessary cellular processes. *ALL organisms use a 2-step process to provide the energy needed for most of their biological processes: 1) Chemical energy from organic molecules like glucose is used to produce ATP in a process called cellular respiration 2) ATP provides energy for most biological processes. The process of Cellular Respiration takes place in the mitochondria!! *Cellular respiration is the process that transfers some of the chemical energy in glucose or another organic molecule to chemical energy in ATP, so energy is available in a form that is useful for biological processes. Cellular Respiration Equations = C6H12O6 + 6O2 6CO2 + 6H2O + Energy Note that not all of the energy released from glucose by cellular respiration is captured in ATP: some of the energy is converted to heat. *To use energy from food: Cellular respiration transfers energy in organic molecules such as glucose to energy in ATP. Then, ATP is used to provide energy for cellular processes. *Fermentation-produces energy from food molecules by producing ATP in the absence of oxygen. It is said to be anaerobic. The 2 main types of fermentation are alcoholic fermentation and lactic acid fermentation. Alcoholic fermentation produces carbon dioxide gas and is the reason bread dough rises. Lactic acid fermentation can cause muscle soreness due to lactic acid build-up in muscle Darland/Spaniel Photosynthesis equation = 6CO2 + 6H2O 6O2 + C6H12O *Photosynthesis begins with light reactions which convert the energy in sunlight to chemical energy in ATP and NADPH. In the 2nd stage of photosynthesis, known as the Calvin Cycle, ATP and NADPH provide the energy and H needed to convert CO2 to a 3-carbon molecule which is converted to glucose. Glucose can also be used to produce starch (a storage molecule) and cellulose (a major structural molecule in plants). The process of Photosynthesis takes place in the chloroplast!! Relating Photosynthesis and Cellular respiration: Plants must also carry out cellular respiration to provide ATP for cellular processes. Notice that the equation for photosynthesis and the equation for cellular respiration are FLIPPED! The reactants become the products and the products become the reactants. Heterotrophs use the process of Cellular Respiration to obtain the energy needed stored in glucose. Autotrophs use the process of Photosynthesis to obtain their energy stored in glucose by making glucose. Darland/Spaniel (Punnett Squares) Monohybrid/Dihybrid crosses Carpenter/Fuller Steps for setting up a Punnett Square 1. Make a Key for trait(s) observed 2. Determine Genotypes of each parent 3. Determine possible gametes of each parent 4. Set up offspring in punnett square 5. Analyze your results Example: A green pea plant (GG) is being crossed with a green pea plant (Gg), yellow is the recessive color. Step 1 Step 2 Key: Genotypes: G= green Parent #1 Gg ParentG#2 gametes G Step 4 Step 3 g= yellow Parent #2 GG Step 3 GG GG Gg Gg Analysis of results Step 5 G Parent #1 gametes g Genotype = 2 GG: 2 Gg ; 0 gg Phenotype = 4 Green pea plants: 0 yellow pea plants DNA Structure In 1953, James Watson and Francis Crick established the structure of DNA (Nucleic Acid). The structure is a double helix, which is like a twisted ladder. The sides of the ladder are made of alternating sugar and phosphate molecules. The sugar is deoxyribose. The rungs of the ladder are pairs of 4 types of nitrogen bases. Two of the bases are purines - adenine and guanine. The pyrimidines are thymine and cytosine. The bases are known by their coded letters A, G, T, C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. This is known as the Base-Pair Rule. The bases can occur in any order along a strand of DNA. The order of these bases is the code that contains the instructions. For instance ATGCACATA would code for a different gene than AATTACGGA. A strand of DNA contains millions of bases. (For simplicity, the image only contains a few.) Note that the bases attach to the sides of the ladder at the sugars and not the phosphate. The combination of a single base, a deoxyribose sugar, and a phosphate make up a nucleotide. DNA is actually a molecule or repeating nucleotides. Examine the nucleotides closer. Two of the bases are purines - adenine and guanine. The pyrimidines are thymine and cytosine. Note that the pyrimidines are single ringed and the purines are double ringed. The two sides of the DNA ladder are held together loosely by hydrogen bonds. Karyotypes: A karyotype is an organized profile of a person's chromosomes. In a karyotype, chromosomes are arranged and numbered by size, from largest to smallest. This arrangement helps scientists quickly identify chromosomal alterations that may result in a genetic disorder. To make a karyotype, scientists take a picture of someone's chromosomes, cut them out and match them up using size, banding pattern and centromere position as guides. Why It Is Done Karyotyping is done to: Determine whether the chromosomes of an adult have an abnormality that can be passed on to a child. Determine whether a chromosome defect is preventing a woman from becoming pregnant or causing miscarriages. Determine whether a chromosome defect is present in a fetus. Karyotyping also may be done to determine whether chromosomal problems may have caused a fetus to be stillborn. Determine the cause of a baby's birth defects or disability. Help determine the appropriate treatment for some types of cancer. Identify the sex of a person by determining the presence of the Y chromosome. This may be done when a newborn's sex is not clear. Carpenter/Fuller Karyotype Station: 1. What is a karyotype? 2. List at least 2 types of information karyotypes provide. 3. How many chromosomes do humans have? 4. Look at the karyotype below. What gender is revealed? 5. Is the karyotype “normal” or “abnormal” in the picture below? Describe how you can tell. 6. Look at the karyotype below. Is the karyotype “normal” or “abnormal” in the picture below? Describe how you can tell. 7. What is the gender of the karyotype below? Carpenter/Fuller DNA Station: 1. What are the sides of the DNA ladder made of? 2. What is the monomer for DNA? 3. What three parts make up a single nucleotide? 4. What are the 4 Nitrogenous bases that make up the rungs of the DNA ladder? 5. What sugar is found in DNA? 6. How do the bases bond together? A bonds with _____ , G bonds with _________ and Vice - Versa. 7. What is the shape of DNA? Genetic Crosses Station: 1. What information does a genetic cross provide? 2. Define: a. Homozygousb. Heterozygousc. Recessived. Dominant- 3. What is the answer to the Genetic Problem #1? List the Genotype(s), Phenotype(s), and percentages. 4. What is the answer to the Genetic Problem #2? List the Genotype(s), Phenotype(s), and percentages. 5. What is the answer to the Genetic Problem #3? List the Genotype(s), Phenotype(s), and percentages.