DETERMINATION OF THIOLS BY
FLUORESCENCE USING AU@AG
NANOCLUSTERS AS PROBES
Zhong-Xia Wang, Shou-Nian Ding*, Ezzaldeen Younes Jomma Narjh
School of Chemistry and Chemical Engineering, Southeast University, Nanjing,
Jiangsu 211189, P. R. China.
*
Corresponding author E-mail: snding@seu.edu.cn
SUPPORTING INFORMATION
Figure S1. Schematic for the determination of biothiols using Au@Ag nanoclusters.
1
Figure S2. (a) Ultraviolet-visible absorption and (b) fluorescence (F) emission spectra
of the Au@Ag nanoclusters. Inset (from left to right): photographs of aqueous
solutions of the Au@Ag nanoclusters under visible and 365 nm light.
Figure S3. (LEFT) Fluorescence (F) photostability of the Au@Ag nanoclusterss (40
µM) as a function of the storage time.
(RIGHT) Fluorescence (F) spectra (λex = 370 nm) in the (a) absence and (b) presence
of 100 µM cysteine. Inset: photographs under 365 nm light of 40 µM Au@Ag
nanoclusters in the (a) absence and (b) presence of 100 µM cysteine.
2
Figure S4. Time-dependent fluorescence (F) response of the Au@Ag nanoclusters
(40 μM) upon addition of 100 μM cysteine.
3
Figure S5. (A) Fluorescence emission spectra of Au@Ag nanoclusters (40 µM) in the
presence of different concentrations of cysteine (0 to 200 μM, excitation at 370 nm).
(B) Plot of the enhanced fluorescence signal [(F0-F)/F0] versus cysteine concentration.
(C) Fluorescence emission spectra of Au@Ag nanoclusters (40 µM) in the presence of
different concentrations of glutathione (0 to 200 μM, excitation at 370 nm).
(D) Plot of the enhanced fluorescence signals [(F0-F)/F0] versus glutathione
concentration. The fluorescence intensities of the Au@Ag nanoclusters in the absence
and presence of thiol-containing amino acids are denoted by F0 and F.
4