Pre-prepared Libraries
QC Guidelines
We expect that your libraries will be supplied in the correct containers and at the
required concentration and quality (requirements are stated below). Timely
completion of your project depends on you following these guidelines.
We perform an in-house QC, in the event that your library does not meet our QC
criteria your project manager will contact you to inform you of the problems. It will
then be at your discretion whether you sequence the library. If you choose not to
sequence the library and instead provide us with a replacement library additional
costs for QC will be levied for each replacement library.
For further details on packing and submitting your libraries, please see the following
document ‘Guidelines for Preparing and Submitting Samples v1.4’.
Library QC
Please check the following guidelines to ensure that your libraries meet our QC
criteria. If you are unsure please contact your project manager prior to sending the
libraries.
Pre-prepared Libraries
When submitting prepared libraries, these must be at least 10nM, when quantified
using fluorescence (see the table below for a guide to the concentrations required).
We require at least 15L of each prepared library.
Average Size of Library (bp)
250
300
400
500
1
Version 1.3
March2015
Minimum Concentration of DNA
(ng/L)
1.7
2
2.6
3.3
Summary Table
Type of
Sample
Prepared
Libraries
Amount
required
~45ng
Concentration Volume
~3ng/μl
15μl
Agarose
gel
Bioanalyzer
DNA 1000
or High
Sensitivity
Chip
N.B. When optimal library concentrations cannot be obtained it may still be
possible to sequence the library but the amount of data cannot be guaranteed.
Please discuss this with your project manager.
2
Version 1.3
March2015