1 Short Communication Title: Identification and topographical characterization of microbial nanowires in Nostoc punctiforme Journal Name: Antonie van Leeuwenhoek Authors: Sandeep Sure · Angel AJ Torriero · Aditya Gaur · Lu Hua Li · Ying Chen · ChandrakantTripathi · AlokAdholeya · M Leigh Ackland · Mandira Kochar Mandira Kochar(corresponding author) TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute, GualPahari, Gurgaon-Faridabad Road, Gurgaon, Haryana 122 001, India Email: mandira.malhotra@gmail.comor mandira.kochar@teri.res.in; Tel. (+91)9711963966; Fax. +9111 24682144 Sandeep Sure·Aditya Gaur·ChandrakantTripathi·AlokAdholeya·Mandira Kochar TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute, GualPahari, Gurgaon-Faridabad Road, Gurgaon, Haryana 122 001, India Angel AJ Torriero·M Leigh Ackland Centre for Cellular & Molecular Biology, Deakin University, 221 Burwood Highway, Burwood, Melbourne, Victoria 3125, Australia. 2 Lu Hua Li·Ying Chen Institute for Frontier Materials, Deakin University, Geelong Waurn Ponds Campus, Geelong,Victoria 3216, Australia. 3 Fig. S1 TEM analysis of Nostoc punctiforme short/thin and long/thick PLS (denoted by closed and open arrow, respectively). (a) Long/thick PLS observed along with short/thin PLS in N. punctiforme, (b) Magnified image of a in the reverse direction showing closely spaced long/thick PLS and short/thin PLS, (c) Magnified image of b showing short/thin PLS; (d-e) N. Punctiforme cells showing long/thick PLS along with short/thin PLS 4 Fig.S2 I-V graph of extracellular material (ECM) attached to the PLS (inset of Fig. 3B). Horizontal noisy line represents non-conductive behaviour of ECM. 5 Fig.S3 Current map images of (a) short/thin and (b) long/thick PLS from N. punctiforme grown under normal culture conditions, at +0.2V, (c) Current map images of I-V spectra of short/thin PLS from N. punctiforme cells grown under continuous light intensity, at +0.2V.