Transient transfection
Table : Transient transfection
Anticipated uses:



Quick production of proteins from a wide range of cell
types.
Stable cell line generation.
Cell-based screening assays.
Mandatory
Requirements
Options
System
System should be
able to perform
simple, easy and
rapid transient
transfection of cell
lines, stem cells and
other difficult to
transfect cells with
DNA, RNA, proteins
or cell lysates using
electroporation or
other transfection
methods.
Ability to do stable
transfection using
the system would
also be desirable.
Cell types
Uses of broad range
of cell type including
CHO cells, HEK cells,
NS0, VERO, insect
and other cell types.
Efficiency and
viability posttransfection
Transfection
efficiencies and cell
viabilities must be
greater than 90%.
Pre-loaded with a
library of
transfection
protocols, each
optimized for a
specific cell type.
Data supported
evidence would be
appreciated.
Reagents
Reagents formulation
should be animal
component free and
no biological agents
or hydrolysates
added unto.
System should be
computer control,
programmable and
faster processing
enabling more
assays.
Automation
Scalability
Consistency
Support
Training
Transfect cells at
small (105 – 107
viable cells/ml) and
large scale (up to 1010
viable cells/ml).
Reproducible results
from run-to-run.
Must be service
engineers available
for help/advice in the
event of the system
malfunctioning. A 3
year maintenance
agreement would
desirable.
Full operator training
on the safe operation
Generic data and
case studies
showing system is
scalable.
Data supported
evidence would be
appreciated.
Answer or Details
Complies?
(Y/N)
Please provide details of your
equipment functionality in
relation to CPI requirements
specified
Please confirm if your
product complies with
CPI's functionality
requirements by
providing a Yes (Y) or No
(N) response
Consumables
Applications
Warranty
of the equipment and
associated software
routine maintenance
and troubleshooting.
The consumable
costs for running up
to 10 transfections for
small and large scale
should be included.
Antibody production,
general protein
production, viral
vector and virus-like
particles, insect cells
and siRNA
transfection.
18 months minimum
warranty
Extended
Warranty