Medical Biochemistry and Molecular Biology Department Chemicals Bottles Used for: Storage of different chemicals, reagents, and even strong corrosives. So, they should always carry an informative label. *Instruments used for handling Chemicals Pipette pump It is inserted into the end of the glass pipette to help the delivery of liquid without suction by mouth. Spatula It resembles small paddles or teaspoons. It resists corrosions. It is used to handle solid powdered chemicals. 1 Medical Biochemistry and Molecular Biology Department Plastic Pasteur transfer Pipette Droppers & Plastic transfer pipette They are used to transfer small amounts or drops of liquid solutions. Test Tube Racks Test tube racks are for holding and organizing test tubes on the laboratory counter. Plastic racks may melt in contact with very hot test tubes. Test Tube Holder A test tube holder is useful for holding a test tube which is too hot to handle. Petri dish Used for culture studies and for preparing agar plates. 2 Medical Biochemistry and Molecular Biology Department Watch Glass Used as a surface to evaporate a liquid. It may be used as a beaker cover or as a weighing tray. Rubber Stoppers Rubber stoppers are used to close containers to avoid spillage, evaporation and /or contamination. Containers should never be heated when there is a stopper in place. Well Plates Well plates are used when we want to perform many small scale reactions at one time. Wash Bottle A wash bottle has a spout that delivers a wash solution to a specific area. Distilled water is the only liquid that should be used in a wash bottle. 3 Medical Biochemistry and Molecular Biology Department Test Tube Brushes Test tube brushes are used to clean test tubes and graduated cylinders. Forcing a large brush into a small test tube will often break the tube. Forceps Forceps (or tweezers) are used to pick up small objects. Weighing Tray Weighing boats are used to weigh solids that will be transferred to another vessel. Bunsen Flame Tripod Bunsen burners are used for the heating of nonvolatile liquids and solids. *Instruments used for Filtration Funnels A funnel is used to aid in the transfer of liquid from one vessel to another. They are also used to separate solids from liquids with the aid of filter papers. 4 Medical Biochemistry and Molecular Biology Department Filter papers *Instruments Used For Detecting pH And Recording of Temperature And Specific Gravity Litmus papers Red litmus paper is used to identify bases. Blue litmus paper is used to identify acids. Thermometer Calibration marks along it, allow the temperature to be read by the length of mercury within it. It is used usually to measure the temperature of water bath in lab. 5 Medical Biochemistry and Molecular Biology Department Urinometer (Hydrometer) Used to measure the specific gravity of a liquid e.g. urine *Apparatuses and their parts Used in Lab Balance Used to weigh chemicals Centrifuge Spins liquid samples at different high speeds to sediment different fractions. Gel Electrophoresis Apparatus: Used to separate macromolecules as DNA , RNA and Protein 6 Medical Biochemistry and Molecular Biology Department Comb of Gel-Electrophoresis Forms wells in the gel which are needed to load samples in them. Spectrophotometer Cuvette Used to measure the absorbance (O.D) of different substances in biological fluid; using it to calculate the corresponding concentrations. Bulb of pH meter pH-Meter Apparatus: Used to measure the pH of a solution. Lab water bath Water Bath provides precise temperature for lab reactions control and is available with an analog or digital operating system Question: Identify the following instruments: A------------------------------B-----------------------------C-------------------------Chose the proper instrument to measure 1l of solution: Answer is----------7