FACULTY MEMBER, PH.D.
1234 Drive, Houston, Texas 77030
phone: xxx-xxxx
Senior Scientist
email: facultymember@gmail.com
Energy Development
Research Management
Broadly-trained scientist with expertise in biochemistry, cell biology, genetics, and molecular biology.
Particularly adept in developing in vitro assay systems that reconstitute complex cellular functions, which can
facilitate assigning orphaned protein function and drug efficacy. Seeking to contribute research talents and
management skills to oversee or direct novel alternative energy research and development.
CAREER ACCOMPLISHMENTS
EDUCATION
B.S.
Microbiology
University of Florida, Gainesville, Florida
• Assisted in developing a rat hepatocyte cell culture system to examine amino acid transport with Dr. Sad
(Department of Biochemistry and Molecular Biology, UF Medical School).
Ph.D.
Biochemistry University of Texas Southwestern Medical Center, Dallas, Texas
• Worked with Dr. Friendly and characterized multiple forms of neprilysin, an integral plasma membrane
endopeptidase that regulates activity of >10 neuronal and hormonal peptides, including  amyloid.
• Developed immuno assay and affinity procedures to detect (ng level) and purify neprilysin iso-forms in mg
quantities.
EXPERIENCE
Postdoctoral
Cell Biology
4/05 to 8/10 California Institute of Technology, Pasadena and
University of California San Diego, La Jolla
• Worked with Dr. Happy, an internationally recognized cell biologist.
• Developed novel methods to permeabilize cells and maintain cellular functions.
• Reconstituted inter-compartmental transport between organelles in permeabilized cells.
• Designed and implemented new methods for subcellular fractionation to discover a new cellular organelle,
the prevacuolar compartment.
• Pioneered the use of a novel vital dye, FM4-64, to explore endocytosis, now used widely.
• Competitively obtained 2 funded fellowships from the American Cancer Society.
Assistant Professor
2010-2013
University of Maryland, Baltimore, MD
• Competitively obtained $1.9 million in research funds from the American Heart Association and the NIH.
• Trained and supervised 3 technical personnel; taught/directed > 40 graduate, medical, or undergraduate
students to produce publishable experiments in biochemistry, cell biology, genetics, and molecular
biology.
• Served and advised on > 12 doctoral graduate student thesis committees.
• Directed a departmental Summer Undergraduate Research Program for 3 years.
• Established for the first time, ATP-binding activity of a protein, Vps33p, that has domains with only weak
homology to known ATP binding motifs.
• Generated a novel 2-fluor cell sorting technique that uses pseudo-FRET to isolate mutant cells.
• Developed the use of an elegant cell lysis procedure to reconstitute protein transport between two organelles
in a cell-free system.
• Invited > 20 times to present research seminars across the US.
• Co-author of 16 publications in high impact, peer-reviewed journals and 3 invited book chapters.
EXPERTISE
designing in vitro systems immunoassays
yeast genetics
digital image analysis
protein expression/purification
subcellular fractionation
grant writing
fluorescence microscopy