1. What was the name of the sheep that was the first mammal cloned from an adult somatic cell? a. Molly b. Polly c. Dolly 2. What is the generation time of the most commonly used bacterial strain for cloning – E. coli? a. 20 seconds b. 20 minutes c. 2 days 3. What do the first three letters of the restriction enzyme acronym EcoRI stand for? a. E. coli (because that is the origin of the enzyme) b. Economical (because it can be used with small reaction volumes) c. Echo (because it makes noises when incubated in LB media at 37°C) 4. What is formed when T4 DNA ligase joins 5’-phosphate and 3’-hydroxyl groups? a. Hydrogen bond b. Phosphodiester bond c. James bond 5. Which statement about Thermo Scientific FastDigest Restriction Enzymes is true: a. They are always delivered with express shipping b. They all work in different buffers and digest DNA in 12-16 hours c. They all work in one buffer and digest DNA in 5-15 minutes 6. In which year were Arber, Smith and Nathans awarded with a Noble Prize for the discovery and innovative work on restriction enzymes? a. 1901 b. 1978 c. 2010 7. Which statement about Thermo Scientific CloneJET PCR Cloning Kit is NOT true: a. It uses a lethal gene for positive selection b. It only works for sticky – end DNA fragments c. It allows cloning of sticky or blunt – end DNA fragments in up to 10 minutes 8. Why is Phusion DNA polymerase considered as the golden standard for cloning applications? a. Because it is extremely accurate b. Becouse it allows c. Becouse it comes with Phusion Gold buffer 9.