Objectives – Cloning/Recombinant DNA
1. Define cloning.
2. Cloning typically falls into three broad categories, reproductive cloning, therapeutic
cloning, and recombinant DNA technology (or DNA cloning). Describe each one of these
(including techniques utilized to accomplish each type). How do they differ from one
another?
3. In reproductive cloning, the “cloned” offspring is not truly identical to the “parent”.
Why is this?
4. How do embryonic stem cells differ from adult stem cells?
5. What is a plasmid?
6. Define genetic transformation.
7. Describe various methods that are available to perform genetic transformation.
8. Describe the components of a plasmid typically utilized in DNA cloning.
9. What are the necessary components found in an expression vector?
10. Describe the technique utilized to “remove” introns from eukaryotic genes in order to
prepare them for insertion into an expression vector.
11. Describe the experimentation which led to the very first transgenic animal.