2015 REGISTERED USER REQUEST FORM BOSCH MOLECULAR BIOLOGY FACILITY REGISTERED USERS ARE DEFINED AS THOSE PEOPLE WHO HAVE COMPLETED THIS REGISTRATION FORM AND AGREED TO PAY THE FULL ACCESS FEE. A REGISTRATION FORM AND ACCESS FEE MUST BE COMPLETED FOR THE MOLECULAR BIOLOGY FACILITY INDEPENDANTLY FROM OTHER FACILITIES FOR EACH USER PER CALENDER YEAR. THOSE USERS FROM SCHOOL OF MEDICAL SCIENCES (SoMS) WILL PAY $318 PER ANNUM. OTHER MEMBERS OF THE UNIVERSITY OF SYDNEY (NON-SoMS MEMBERS) WILL PAY AN ACCESS FEE OF $473. ALL OTHERS MUST PAY THE EXTERNAL USER FEE OF $1050. NOTE: ALL REGISTERED USERS MUST UNDERTAKE APPROPRIATE TRAINING BEFORE USING THE FACILITY – DETAILS CAN BE OBTAINED FROM THE CORE FACILITY OFFICERS. IN THE EVENT THAT SOMEONE IS FOUND TO HAVE MISUSED THE FACILITY, ACCESS MAY BE WITHDRAWN. FOR FULL DETAILS ON ACCESS FEES AND THE USAGE OF BOSCH CORE FACILITIES PLEASE READ “GUIDELINES FOR THE USAGE OF BOSCH CORE FACILITIES”. Parties to this Agreement Registration Details (Please Print) Title: _____ Surname: ___________________________ Given Name: ______________________________ Staff/Student ID:__________________ Position: __________________________________________ Bosch Research Laboratory: _______________________________________________________________ [Note only those listed on the SoMS Directory will be counted as SoMS Members for the purpose of determining Access Fees] Or External Address: ______________________________________________________________________ Contact No: ____________________ Email: _______________________________________________ Duration of access: ________________ to 31 December 2015 Name of Supervisor: ________________________________________________________________ Have you Completed the Training Requirements?: Date Training Completed: _____________ Yes No (Please tick)) Details: _______________________________________ Annual Fee for SoMS member is $318 per Core Facility. Annual Fee for other University of Sydney members is $473 per Core Facility. Annual Fee for External users is $1050 (excl. GST) per Core Facility. Fees run for Calendar Year. Access Fee Payment - Journal Transfer (Please print details) I ______________________________ from the Discipline of _______________________________________ Endorse the journal transfer of from my account code: Amount: (Please tick) $318 (Members of SoMS) $473 (other University of Sydney member) $1050 (Non-University member) There is 40% discount for registrations made after July 1st Journal transfer RC: __ __ __ __ __Project: __ __ __ __ __ Analysis: __ __ __ __ __ __ __ __ Invoice please Being 2015 Access Fee for the Bosch Molecular Biology Facility for: Registered User: _______________________________________________________________________ Signed (Supervisor, HOD etc): _______________________________________Date:_________________ To be completed by the Bosch Molecular Biology Officer: Date Sent to Accounts: Conditions of Use ________________ Date Completed: _________________ 1. The Registered User will have access to the Bosch Molecular Biology Facility in both the Blackburn Building and the Anderson Stuart Building. 2. The Registered User must undertake appropriate training with the Molecular Biology Officer (MBO) prior to using specialised equipment and/or areas. 3. The Registered User must at all times follow all rules and procedures for the use of the facility including the wearing of any required safety clothing. 4. The Registered User will be responsible for the general maintenance and cleanliness of the equipment and areas being used including the safe disposal of any waste materials. 5. The Registered User must report any accidents or misuse of the facility to the MBO, Dr Donna Lai or Dr. Sheng Hua on 9351 8787 or 9351 4576 or 0403 151 568 (Donna), 0405 382 568 (Sheng). 6. The Registered User must not allow access to non-registered users. 7. Prior to the commencement of access, the Registered Users must have completed and have a delegated authorised signature on the Access Fee Payment – Journal Transfer Form. 8. In the event that you breach any of the obligations under this Agreement, the Bosch Institute is entitled to terminate this agreement without notice. 9. This Bosch Core Facility should be acknowledged on all publications and presentations resulting from its use, and, if appropriate, the Core Officer should also be acknowledged. Agreement Agreement I agree to the above terms and conditions. Name_______________________ Signed______________________ Date_____________ Thank You EQUIPMENT USED Please tick the appropriate box(es). Aglient Bioanalyzer 2100 (AS) NanoDrop spectrophotometers (AS, BB) C1 Single-Cell Auto Prep System (BB) Qubit 2.0 Fluorometers (AS, BB) GeneAtlas microarray system (AS) PCR machines: TC5000, Hybaid or T100 (AS, BB) Roche LightCycler 480 qPCR (AS, BB) Rotor Gene 3000 or 6000 qPCR machines Tecan EVO75 robotic system (BB) QuantStudio 12K Flex real time PCR system (AS) GenomeLab GeXP Genetic Analysis system Advalytix AmpliSpeed slide cycler Luminex 200 systems (AS, BB) Licor Odyssey Imaging System (BB) Syngene G:Box Digital Imaging System (BB) Q24 PyroMark System (BB) Seahorse XF Analyzers (AS) ChemiDoc MP Digital Imaging System (AS, BB) BMG microplate readers (AS, BB) Partek data analysis and Pathway software (AS) Cytation 3 cell imaging system (BB) GenEX qPCR analysis software dongle Agarose gel electrophoresis systems QX100 Droplet Digital PCR system (BB) Direct Detect Spectrometer (AS, BB) Muse Cell Analyser (AS) MicroBeta TriLux Scintillation Counter (BB) Packard TRI-CARB Liquid Scintillation Analyzer Countess Automated Cell Counter (BB) SDS-PAGE gel electrophoresis, Western Blotting & SNAP i.d. protein detection system (AS, BB) Spectrophotometers (AS, BB) Peggy Automated Western Blotting System (AS) Biosafety Cabinets (AS, BB) CO2 Incubators (AS, BB) Incubator with O2 level Control (AS) BLItz Protein analysis system (AS) TC10 Automated Cell Counter (AS) Beckman Coulter ultra- and high-speed centrifuges (L80XP, TLX-120, Avanti J-E) (BB) IncuCyte ZOOM live cell imaging systems Avestin emulsiflex-C5 homogeniser(BB) Freeze Dryer (AS) Christ RVC2-18 vacuum-concentrator (BB) Primer Express 3.0.1 (AS) Benchtop Bucket Centrifuges (AS, BB) Autoclaves (AS, BB) NanoSight LM10-HSBFT14 (AS) Inverted microscopes and camera (AS, BB) Shaking incubators (AS, BB) MilliQ water systems (AS, BB) Bio-Rad Micropulse Electroporator (AS) Tharmac Cytocentrifuge Cellspin (BB) FPLC machine (BB) QIAgility Robot (BB) xCelligence SP or DP systems Other (please specify) If you don’t know how to use any equipment and you would like to be trained, please contact Dr. Donna Lai or Dr. Sheng Hua. Online booking calendars or sign-up sheets for major equipment are available. Please make sure that you book the equipment online before or fill out sign-up sheets when you start using them. Organisms you are planning to bring into the facility: Genetically Modified Organisms (GMOs) you are planning to bring into the facility: Procedures/Research projects you are planning to conduct within the facility: