2011 REGISTERED USER REQUEST FORM BOSCH MOLECULAR BIOLOGY FACILITY REGISTERED USERS ARE DEFINED AS THOSE PEOPLE WHO HAVE COMPLETED THIS REGISTRATION FORM AND AGREED TO PAY THE FULL ACCESS FEE. A REGISTRATION FORM AND ACCESS FEE MUST BE COMPLETED FOR EACH USER FOR THE ADVANCED MICROSCOPY AND MOLECULAR BIOLOGY FACILITIES INDEPENDENTLY FOR EACH CALENDER YEAR. THOSE USERS FROM BOSCH INSTITUTE OR FACULTY OF MEDICINE WILL PAY $300 PER ANNUM. OTHER MEMBERS OF THE UNIVERSITY OF SYDNEY (NON-BOSCH MEMBERS) WILL PAY AN ACCESS FEE OF $450. ALL OTHERS MUST PAY THE EXTERNAL USER FEE OF $1000. NOTE: ALL REGISTERED USERS MUST UNDERTAKE APPROPRIATE TRAINING BEFORE USING THE FACILITY – DETAILS CAN BE OBTAINED FROM THE CORE FACILITY OFFICERS. IN THE EVENT THAT SOMEONE IS FOUND TO HAVE MISUSED THE FACILITY, ACCESS MAY BE WITHDRAWN. FOR FULL DETAILS ON ACCESS FEES AND THE USAGE OF BOSCH CORE FACILITIES PLEASE READ “GUIDELINES FOR THE USAGE OF BOSCH CORE FACILITIES”. Parties to this Agreement Registration Details (Please Print) Title: _____ Surname: ___________________________ Given Name: ______________________________ Staff/Student ID:__________________ Position: __________________________________________ Bosch Research Laboratory: _______________________________________________________________ [Note only those listed on the Bosch Directory will be counted as Bosch Members for the purpose of determining Access Fees] Or External Address: ______________________________________________________________________ Contact No: ____________________ Email: _______________________________________________ Duration of access: ________________ to 31 December 2011 Name of Supervisor: ________________________________________________________________ Have you Completed the Training Requirements?: Date Training Completed: _____________ Yes No (Please tick)) Details: _______________________________________ Annual Fee for Bosch Member or Faculty of Medicine member is $300 per Core Facility. Annual Fee for other University of Sydney members is $450 per Core Facility. Annual Fee for External users is $1000 (excl. GST) per Core Facility. Fees run for Calendar Year. Access Fee Payment - Journal Transfer (Please print details) I ______________________________ from the Discipline of _______________________________________ Endorse the journal transfer of from my account code: Amount: (Please tick) $300 (member of Bosch or Faculty of Medicine) $450 (other University of Sydney member) $1000 (Non-University member) Journal transfer RC: __ __ __ __ __Project: __ __ __ __ __ Analysis: __ __ __ __ __ __ __ __ Invoice please Being 2011 Access Fee for the Bosch Molecular Biology Facility for: Registered User: _______________________________________________________________________ Signed (Supervisor, HOD etc): _______________________________________Date:_________________ To be completed by the Bosch Molecular Biology Officer: Date Sent to Accounts: ________________ Date Completed: _________________ Conditions of Use 1. The Registered User will have access to the Bosch Molecular Biology Facility in both the Blackburn Building and the Anderson Stuart Building. 2. The Registered User must undertake appropriate training with the Molecular Biology Officer (MBO) prior to using specialised equipment and/or areas. 3. The Registered User must at all times follow all rules and procedures for the use of the facility including the wearing of any required safety clothing. 4. The Registered User will be responsible for the general maintenance and cleanliness of the equipment and areas being used including the safe disposal of any waste materials. 5. The Registered User must report any accidents or misuse of the facility to the MBO, Dr Donna Lai on 9351 8787 or 9351 4576 or 0403151568. 6. The Registered User must not allow access to non registered users. 7. Prior to the commencement of access, the Registered Users must have completed and have a delegated authorised signature on the Access Fee Payment – Journal Transfer Form. 8. In the event that you breach any of the obligations under this Agreement, the Bosch Institute is entitled to terminate this agreement without notice. 9. This Bosch Core Facility should be acknowledged on all publications and presentations resulting from its use, and, if appropriate, the Core Officer should also be acknowledged. Agreement Agreement I agree to the above terms and conditions. Name_______________________ Signed______________________ Date_____________ Thank You EQUIPMENT USED Please tick the appropriate box(es). Aglient Bioanalyzer 2100 (AS) Hybaid or Palm Cycler PCR machines (AS) TC5000 PCR machine (BB) Rotor Gene 3000 or 6000 qPCR machines Roche LightCycler 480 qPCR machines NanoDrop spectrophotometers (AS, BB) Tecan EVO75 robotic system QIAgility Robot (BB) GenomeLab GeXP Genetic Analysis system Advalytix AmpliSpeed slide cycler Luminex 200 systems (AS, BB) Licor Odyssey Imaging System (BB) Syngene G:Box Digital Imaging System (BB) Alpha Innotech Digital Imaging System (AS) BMG microplate readers (AS, BB) Agarose gel electrophoresis Seahorse XF Analyzer (AS) Spectrophotometers (AS, BB) MicroBeta TriLux Scintillation and Luminescence Counter (BB) SDS-PAGE gel electrophoresis, Western Blotting & SNAP i.d. protein detection system (AS, BB) Countess Automated Cell Counter (BB) TC10 Automated Cell Counter (AS) Biosafety Cabinets (AS, BB) CO2 Incubators (AS, BB) Beckman Optima TLX120 ultracentrifuge(BB)Beckman Optima L80XP ultracentrifuge Avanti J-E high speed centrifuge (BB) Avestin emulsiflex-C5 homogeniser(BB) Freeze Dryer (W312, AS) Christ RVC2-18 vacuum-concentrator (BB) Microfuge 22R centrifuge (BB) Allegra X-12R centrifuges (AS, BB) Eppendorf benchtop Centrifuges (AS) Sigma 4K15 centrifuge (AS) Autoclaves (AS, BB) FPLC machine (BB) Inverted microscopesand camera MilliQ water systems (AS, BB) Other (please specify) Shaking incubators (AS, BB) Bio-Rad Micropulse Electroporator (AS) If you don’t know how to use any equipment and you would like to be trained, please contact Dr. Donna Lai. Online booking calendars or sign-up sheets for major equipment are available. Please make sure that you book the equipment online before or fill out sign-up sheets when you start using them. Organisms you are planning to bring into the facility: Genetically Modified Organisms (GMOs) you are planning to bring into the facility: Procedures/Research projects you are planning to conduct within the facility: