BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Diane S. Lidke
Associate Professor
eRA COMMONS USER NAME (credential, e.g., agency login)
DSLIDKE
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and
residency training if applicable.)
DEGREE
INSTITUTION AND LOCATION
MM/YY
FIELD OF STUDY
(if applicable)
Nebraska Wesleyan University, Lincoln,
Nebraska
University of Minnesota, Minneapolis, Minnesota
Max Planck Institute for Biophysical Chemistry
Goettingen, Germany
B.S.
1994
Ph.D.
2002
Postdoc
2002-2005
Physics
Biophysical Sciences &
Medical Physics
Fluorescence Microscopy/
Cell Signal Transduction
A. Personal Statement
The overall theme of my research is the application of fluorescence microscopy and biophysical techniques to
the study of cell signal transduction. This involves the study of the spatial-temporal organization of membrane
proteins and how these are altered in diseased cells or influenced by therapeutics. In particular, we study the
erbB family of receptor tyrosine kinases that is implicated in many kinds of cancer. My expertise in live cell
imaging includes techniques such as FRAP, FCS, FRET, hyperspectral imaging and single particle tracking as
well as development of techniques for live cell labeling of proteins of interest with fluorescent protein and/or
quantum dot probes. I also work with members of the UNM Spatiotemporal Modeling Center to integrate
quantitative measurements of biological processes into modeling platforms of signal transduction.
B. Positions and Honors
Professional Appointments
2005-2006
Research Asst. Prof., Department of Pathology, University of New Mexico School of Medicine
2006-2012
Assistant Professor, Department of Pathology, University of New Mexico School of Medicine
2012-present Associate Professor, Department of Pathology, University of New Mexico School of Medicine
Honors and Awards
2002-2005
Max-Planck-Gesellschaft Fortbildungsstipendium, Post-doctoral Fellowship
2006
Selected participant in the UK-Germany Frontiers of Science Symposium
2008-2011
Human Frontiers Science Program Young Investigator Award
2009-2014
NSF Faculty Early Career Development (CAREER) Award
2010
UNM-HSC Jr. Faculty Excellence in Research Award
2011
Biophysical Society’s Margaret Oakley Dayhoff Award
Reviewer for National and International Funding Agencies. 2010 NSF Cellular Organization review panel;
2011-2012 NSF Membrane Trafficking and Biogenesis review panel; Ad hoc reviews for NSF (2009 – present)
Ad-hoc Reviewer for Peer Reviewed Journals. ACS Nano, Molecular Biology of the Cell, Biophysical
Journal, Nano Letters, Journal of Microscopy, PNAS, Journal of Biological Chemistry, Journal of Cell Science,
Blood, PLOS One
Memberships. Biophysical Society 1998-present; ACSB (2010-present); AAAS (2010-present).
C. Selected Peer-reviewed Publications
Most relevant to the current application
1. D.S. Lidke, P. Nagy, R. Heintzmann, D.J. Arndt-Jovin, J.N. Post, H. Grecco, E.A. Jares-Erijman, and T.M.
Jovin. “Quantum Dot ligands provide new insights into receptor-mediated signal transduction.” Nature
Biotechnology, 22: 198 (2004)
2. D.S. Lidke, K.A. Lidke, B. Reiger, T.M. Jovin and D.J. Arndt-Jovin. “Reaching out for signals: filopodia
sense EGF and respond by directed retrograde transport of activated receptors.” Journal of Cell Biology,
170: 619 (2005) PMC2171515
3. N.L. Andrews, K.A. Lidke, J.R. Pfeiffer, A.R. Burns, B.S. Wilson, J.M. Oliver and D.S. Lidke. “Actin restricts
FcεRI diffusion and facilitates antigen induced immobilization.” Nature Cell Biology, 10:955-963 (2008)
PMC3022440
4. N.L. Andrews, J.R. Pfeiffer, A.M. Martinez, D.M. Haaland, R.W. Davis, T. Kawakami, J.M. Oliver,
B.S. Wilson and D.S. Lidke. “Small, mobile FcεRI aggregates are signaling competent.” Immunity, 31: 469479 (2009) PMC2828771
5. S.T. Low-Nam, K.A. Lidke, P.J. Cutler, R.C. Roovers, P.M.P. van Bergen en Henegouwen, B.S. Wilson,
D.S. Lidke. “ErbB1 dimerization is promoted by domain co-confinement and stabilized by ligand.” Nature
Structural & Molecular Biology, 18: 1244-1249 (2011) PMC3210321
Additional recent publications of importance to the field (in chronological order)
1. H.E. Grecco, K.A. Lidke, R. Heintzmann, D.S. Lidke, C. Spagnuolo, O.E. Martinez, E.A. Jares-Erijman and
and T.M. Jovin. “Ensemble and single particle photophysical properties (two-photon excitation, anisotropy,
FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells.” Microscopy
Research and Technique, 65: 169-179 (2004)
2. A. Cambi, D.S. Lidke, D.J. Arndt-Jovin, C.G. Figdor and T.M. Jovin. “Ligand-conjugated quantum dots
monitor antigen uptake and processing by dendritic cells.” Nano Letters, 7:970 (2007)
3. S. Yang, M. Raymond-Stintz, W. Ying, J. Zhang, D.S. Lidke, S.L. Steinberg, J.M. Oliver and B.S. Wilson.
“Mapping erbB receptors on breast cancer cell membranes during signal transduction.” Journal of Cell
Science, 120:2763-2773 (2007)
4. M. Xue, G. Hsieh, M. Raymond-Stintz, J. Pfeiffer, D. Roberts, S.L. Steinberg, J.M. Oliver, E.R. Prossnitz,
D.S. Lidke, and B.S. Wilson. “Activated FPR and FcRI Occupy Common Domains for Signaling and
Internalization.” Molecular Biology of the Cell, 18:1410-1420 (2007) PMC1838997
5. J.L. Smith, D.S. Lidke, M. Ozbun. “Virus activated filopodia promote human papillomavirus type 31 uptake
from the extracellular matrix.” Virology, 381:16-21 (2008) (cover) PMC2862147
6. D.M. Haaland, H.D.T. Jones, M.H. Van Benthem, M.B. Sinclair, D.K. Melgaard, C.L. Stork, M.C. Pedroso,
P. Liu, A.R. Brasier, N.L. Andrews, and D.S. Lidke. “Hyperspectral confocal fluorescence imaging:
Exploring alternative multivariate curve resolution approaches.” Applied Spectroscopy. 63: 271-279 (2009)
7. D.S. Lidke and B.S. Wilson. “Caught in the act: quantifying protein behavior in living cells.” An invited (and
peer-reviewed) review for Trends in Cell Biology, 19: 566-574 (2009) (cover) PMC2783887
8. D.S. Lidke, F. Huang, J.N. Post, B. Rieger, J. Wilsbacher, J.L. Thomas, J. Pouysségur, T.M. Jovin and P.
Lenormand. “ERK nuclear translocation is dimerization-independent but controlled by the rate of
phosphorylation.” Journal of Biological Chemistry.
285:3092-3102 (2010)
PMC2823437
*Selected “Paper of the Week”
9. A. Carroll-Portillo, K. Spendier, J. Pfeiffer, G. Griffiths, H. Li, K.A. Lidke, J.M. Oliver, D.S. Lidke, J.L.
Thomas, B.S. Wilson, J.A. Timlin. “Formation of a mast cell synapse: FcεRI membrane dynamics upon
binding mobile or immobilized ligands on surfaces.” Journal of Immunology, 184: 1328-1338 (2010)
PMC2944771
10. S. de Keijzer, M. Meddens, D. Kilic, B. Joosten, I. Reinieren-Beeren, D.S. Lidke, S. Grinstein and A.
Cambi. “Interleukin-4 alters early phagosome phenotype by modulating class I PI3K dependent lipid
remodeling and protein recruitment.” PLoS ONE. 6(7): e22328. doi:10.1371/journal.pone.0022328 (2011)
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D. Research Support
Ongoing Research Support
1R01GM100114 (Lidke)
5/1/12 – 2/28/17
2.40 CM
NIH/NIGMS
$1,439,070
Single Molecule Imaging to Quantify FcRI Signaling Dynamics
The major goals of this project are to quantify the protein interactions and dynamics at the molecular level
using innovative imaging techniques to further the fundamental understanding of mast cell signaling.
Role: PI
RGY Program Grant (Garcia-Parajo, Lidke)
9/1/12 - 8/31/15
0.60 CM
Human Frontiers Science Program
$100,000
Nano-Mechano-Biology: spatiotemporal remodeling of membrane nanoplatforms under mechanical forces
The major goal of this project is to elucidate the influence of mechanical stress on membrane architecture, cell
signaling and physiological response in Dendritic Cells.
Role: Co-PI
MCB-0845062 CAREER Award (Lidke)
1/1/09-12/31/14
3.00 CM
NSF
$95,541
Elucidating the regulation mechanisms of erbB signaling using quantitative imaging
The major goals of this project are to compare the behavior of wild type and mutant erbB1 using fluorescence
microscopy techniques.
Role: PI
P50GM085273 (Oliver)
8/1/09-7/31/14
2.40 CM
NIH/NIGMS
$2,262,170
New Mexico Center for the Spatiotemporal Modeling of Cell Signaling
Study of cell signaling using computational modeling and experimental techniques.
Role: Co-director of the Super-resolution Imaging Core and Quantitative Measurement Core.
K25CA131558/09-059-UNM (Halasz)
1/1/08-12/31/13
0.60 CM
Sub-award from NIH Grant to Halasz
$13,622
Spatial Monte Carlo models for VEGF binding on the cell membrane
This grant is to measure and model the spatiotemporal regulation of VEGF signaling. The UNM subaward is for
SPT experiments to gather information about VEGFR spatial dynamics.
Role: Co-investigator
5R01AI051575-07 (Wilson)
2/15/09 - 1/31/14
0.60 CM
NIH/NIAID
$279,825
Mapping for Special Domains for FcεRI Signaling & Internalization
This project focuses on composition of membrane microdomains associated with immunoreceptor signaling
Role: Co-investigator
5R01CA119232-05 (Wilson)
7/1/09 – 6/30/13
0.60 CM
NIH/NCI
$211,321
MSM Mapping & Modeling ErbB Receptor Membrane Topograpy
This project is aligned with the IMAG/MSM program; it involves quantitative measurements and mathematical
modeling of membrane signaling proteins, then extends to tumor modeling based upon data from xenografts.
Role: Co-investigator
1R01CA132136-01 (Ozbun)
12/1/08–11/30/12
0.60 CM
NIH
Entry of oncogenic HPVs into human keratinocytes
This goal of this project is to elucidate the mechanisms of HPV host entry.
Role: Co-investigator for imaging.
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Research Support Completed During the Last Three Years
RGY0074/2008 Young Investigator Award (Lidke)
9/1/08 - 8/31/11
Human Frontiers Science Program
Integrating Single Molecule Imaging Techniques to Unravel Mast-Dendritic Cell Interplay
This project is to characterize the potential mast cell – dendritic cell synapse.
Role: PI
RR024438 (K. Lidke)
2/1/09–12/31/11
NIH/NCRR
A hyperspectral microscope optimized for spectrally multiplexed live cell imaging
A fast, line-scanning spectral microscope for multi-color quantum dot single particle tracking.
Role: Co-investigator
Oxnard Foundation Award (Lidke)
2/1/07-1/31/09
Understanding ErbB Signaling in Cancer
This internal grant provided pilot funding for the development of fluorescent probes and single molecule
techniques to characterize erbB receptor dynamics.
Role: PI
Sub-award from American Cancer Society IRG-92-024 (J. Oliver)
4/1/08-3/31/09
Real-time imaging of erbB behavior in primary cancer cells
This internal grant provided pilot funding for development of imaging primary tumor tissue.
Role: PI
Sandia-University Research Program (Lidke)
10/1/06 -9/31/08
Hyperspectral Imaging and Multi-color Single Particle Quantum Dot Tracking
This funding from Sandia National Laboratories supported a collaboration with Sandia scientists to apply
Sandia’s hyperspectral microscope to the study of single molecule imaging and the relation of receptor motion
to signaling.
Role: PI
Sub-award from NIH/NTCNP/Grant 1-U54-RR022241 (PI: A. S. Waggoner, CMU) under the "Driving Biological
Projects" mechanism.
8/01/08- 07/31/10
The influence of subunit integrity and TLR4 crosstalk on IgE receptor signaling (Lidke)
Single particle tracking of two-color fluorogen activating peptides will be used to monitor IgE stability and IgETLR4 interactions.
Role: Co-PI
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