GenCRISPR™ Custom Cell Line Development Service
SC1652 (Knock-out), SC1663 (Knock-in)
Instructions
1. Please complete and email this form to bioassay@genscript.com
2.
Our Account Manager will contact you with a quote.
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or
Account No.
Name:
Account No.:
Phone:
Organization:
Shipping Address:
(Necessary to determine shipping cost)
Email Address:
Genomic Editing
Knock out or
Knock-in/Mutation by Design
(If knock-in/mutation by design service is required, please fill out knock-in/mutation by design section on page 2)
Name of Target Gene (NCBI Accession Number):
DNA Sequence for Targeting
Does KO/KI of the target gene affect cell growth?
Yes
No
Not sure
If yes, how to rescue the KO/KI clones:____________________________________________________________
Does KO/KI of the target gene affect cell survival?
Yes
No
Not sure
If yes, how to rescue the KO/KI clones:____________________________________________________________
Host Cell Line
Name of Host Cell Line:
Who provides host cell line?
GenScript (Note: Extra fee will be charged)
Client (Note: Mycoplasma free certificate will be required)
Growth condition of host cell line?
Adherent
Suspension
Both
Please provide cell doubling time:____________________________________
Resistance of host cell line?
G418
Puromycin
No resistance.
Zeocin
Hygromycin B
Blasticidin S
Other___________
Will serial dilution affect cell growth rate?
Yes
No
Not sure
What are the medium and additives for cell growth?
Medium:______________________________________________________
Additives:_____________________________________________________
What is the suggested method for cell transfection?
Chemical transfection, please specify the reagent:_________________
Electroporation
Nucleofection
Please provide transfection efficiency (if possible):____________________
Do you need GenScript to follow any special cell culture routine?
Yes, see below
No
Please provide protocol with information about the cell line and any special growth characteristics or requirements.
Do the cells contain any human pathogen?
Yes, please specify:___________________________
No
Requirements for Customized Transgenic Cell Line:
Genotype of transgenic cell line:
Homozygous
Heterozygous
Please indicate the preferred type of analysis to characterize transgenic cell line:
Growth curve
Western blot
ELISA
Others, please specify_________________________
Do you need GenScript to analyze the transgenic cell line by special assay?
Yes, please specify_____________________
No
What is the final application of the transgenic cell line?
Gene function analysis
Assay development
Drug screening
Other
If Other, Please indicate your specific application and requirements:_________________________________
Comments:
Gene Name for Knock-in/Mutation by Design:
Gene Accession Number:
For Knock-in/Mutation
by Design Services
DNA Sequence:
Reporter/Tag:
N- or C-terminal:
Selection Marker:
Project Information
Is this project for grant application purpose?
Yes
No
When will the project start?
Immediately
Within one month
Within three months
Half a year later