Department of Physiology
Procedure No:
PHY/SOP/EQ06
Revision No:
001
Effective Date :
17/6/2010
Page:
Page 1 of 2
Safety & Health
Laboratory (Location):
Cytokine biology Lab, MD9
Title:
Cold Room
Prepared/Review By
Approved By
Next Review Date
Oh Chu Yun
Dr. Leung Pui Lam Bernard
16/6/2013
* Review Date = Future date for the next revision (every 3yrs)
1.
OBJECTIVE
This procedure outlines the operation and maintenance of the Cold Room used for doing
work in 4oC in Department of Physiology, NUS.
2.
SCOPE
This SOP is applicable to all Post Doc Researchers, Lab Technologists and Graduate
Students.
3.
4.
RESPONSIBILITY
3.1
It is the responsibility of the PI in conjunction with the laboratory supervisor to ensure
that the Cold Room condition is regularly maintained, in certified good working order
and available for research personnel as requested.
3.2
It is the responsibility of the PI in conjunction with the laboratory in charge to ensure
that all research and technical staff using this facility are adequately trained and
experienced.
PROCEDURES
4.1
4.2
4.3
4.4
Keep all your samples within the shelf allocated to you.
Label all sample boxes, as all unlabelled boxes will be discarded during clean up.
Wear a jacket if you are working in the cold room for more than 5 minutes.
Use only cold proof equipment inside the cold room.
Department of Physiology
Procedure No:
PHY/SOP/EQ06
Revision No:
001
Effective Date :
17/6/2010
Page:
Page 2 of 2
Safety & Health
Laboratory (Location):
Cytokine biology Lab, MD9
Title:
Cold Room
5.
SAFETY PRECAUTIONS
5.1
5.2
5.3
5.4
5.5
5.6
5.7
6.
MAINTENANCE
6.1
6.2
6.3
7.
Know about and use of the luminous knob inside the cold room during emergency to
open the door.
Do not stay in the cold room longer than necessary unless you are wearing a jacket
to prevent developing of hypothermia.
Store all heavier items/stock in the lower shelf.
Beware that condensation occurs while removing equipment from the cold room to
outside environment could cause electrical short circuit.
Clean and dry all spills on floor immediately to avoid being slipped.
Discard all moist cardboard boxes immediately as moisture built up in the cold room
can cause growth of moulds/fungus.
Report all problems (temperature, spaces constraint, mechanical) to the person in
charge immediately.
The person in charge for the cold room is indicated on the door of the cold room.
In case of power trip, please call 65161616 for OED support.
Clean the cold room interior and shelf with 70% alcohol.
REFERENCES
7.1
Refer to manufacturer’s instruction manual.