Supporting Information 1. HPLC on TSK-GELR G2500PWXL® columns of various NDO after incubation with PAA/AMG and protease according to the Integrated RINTDF method described in this paper [2.2.4]. Parallel samples of oligosaccharides (blanks) were passed through the complete method, but were neither incubated with the enzymes nor at elevated temperatures. a) Fibersol 2® ; b) Polydextrose®. The bars in the figure show the fractions included as NDO. 2. HPLC on TSK-GELR G2500PWXL® columns of various NDO after incubation with PAA/AMG and protease according to the Integrated RINTDF method described in this paper [2.2.4]. Parallel samples of oligosaccharides (blanks) were passed through the complete method, but were neither incubated with the enzymes nor at elevated temperatures. c) galacto-oligosaccharides; d) xylo-oligosaccharides; The bars in the figure show the fractions included as NDO. 3. HPLC on TSK-GELR G2500PWXL® columns of various NDO after incubation with PAA/AMG and protease according to the Integrated RINTDF method described in this paper [2.2.4]. Parallel samples of oligosaccharides (blanks) were passed through the complete method, but were neither incubated with the enzymes nor at elevated temperatures. e) raffinose and f) isomalto-oligosaccharides (AdvantaFiber®). The bars in the figure show the fractions included as NDO. 4. HPLC on a Waters Sugar-Pak column of the SDFS fraction obtained from cassava starch incubated according to AOAC Method 2009.01 and the new DF assay format (RINTDF). The arrow shows the point of elution of the resistant heptasaccharide.