Hands-on-skills training on the use of high performance liquid
chromatography (HPLC)
co-hosted by Departments of Agricultural & Biological Sciences
(Uganda Christian University) and Food Technology & Nutrition
(Makerere University)
1st to 10th February 2016
Date
Mon 01/02/16 – day 1
Tues 02/02/16 – day 2
Wed 03/02/16 – day 3
Thurs 04/02/16 – day 4
Fri
05/02/16 – day 5
Sat 06/02/16
Sun 07/02/16
Mon 08/02/16 – day 6
Tues 09/02/16 – day 7
Wed 10/02/16 – day 8
Time
8:00 -9:00
Topic
 View/ assess operation of Perkin Elmer Flexar LC HPLC by
Dr. Thomas Gurley (Full bright Fellow)
 Preparatory logistics
9:00-10:00
10:00-12:30
Opening session
 Introductions
 Fundamental Theory of HPLC Separation
13:30-17:00
9:00-12:30
13:30-17:00
9:00-12:30
13:30-17:00
9:00-12:30
13:30-17:00
9:00-12:30
13:30-17:00
Fundamental Theory of HPLC Separation
HPLC Stationary Phases
HPLC Stationary Phases
Role of Mobile Phase
Role of Mobile Phase
Method Development Strategy
Method Development Strategy
Method Development Strategy
Method Development Strategy
9:00-12:30
13:30-17:00
9:00-12:30
13:30-17:00
9:00-12:30
Practical Operation
Practical Operation
Practical Operation
Practical Operation
Method Development Practicum
13:30-16:00
16:00-18:00
Method Development Practicum
Closing session (includes awards of certificates of participation)
 12:30-13:30 hr – lunch time – Restaurant at Food Tech, Nutrition & Bio-engineering
School (Makerere University)
 15-20 min tea/ coffee breaks will be agreed on each day – preferably working teas