Utmitha Konda WEEKLY JOURNAL Monday: 11/15/10 1. 2. 3. 4. Injected the sample (ClNIHa-demethyl) into the manual HPLC Connected the peaks Rotavapored the product peak Ran an NMR Tuesday: 11/16/10 1. 2. 3. 4. 5. 6. Set up another ClNIHa+HCl reaction this time used a 100mg Transferred into microwave tube Ultrasound it for 20 min until solution was almost soluable Microwaved it at 150C Finally, ran a HPLC HPLC showed completed reaction- placed the tube into the fridge Wednesday 11/17/10 1. 2. 3. 4. Changed the pH of the reaction to about 5 using sodium carbonate Extracted the compound using ethyl acetate and water about three times Filtered using magnesium sulfate Rotavapored until compound was dry Thursday 11/18/10 1. 2. 3. 4. 5. 6. Used DMF to inject some of the dry sample from yesterday into the manual HPLC Connected the peaks There were two peaks Rotavapored the first peak and the second peak First peak was the compound Second peak was the incomplete reaction (have to set up the reaction from step one using this sample.) Friday 11/19/10 My mentor let me stay at school and get help after school because I was getting behind in some of my classes This week’s experiments were repeats. I had to run reactions with smaller number of starting material first and see how the reaction completes and see if the product forms. When I found out that this reaction was successful, I had to run it again and again with a larger amount of starting material. I feel like because I had to run with a large amount of material the reaction did not complete fully under the same conditions as the smaller amount of starting material. This week was very busy for me at school. I had to complete IB internals for the IB exam in May and I had so many tests to study for. I feel like I never I had time to stop. I was either at school, work, or at home doing homework. I even had to complete applications for college. I used to think that senior year was going to be pretty relaxing but it is turning out to be not as easy as I thought it was going to be. Even when I am at work I feel like I am always thinking about what homework I have or what I have to complete. Everyone in the lab always tells me that they wish that they could be doing something as cool as I am with the internship when they were in high school. This makes me feel proud of myself for achieving this internship.