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Using 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in
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flow through biofilters - initial adhesion and BAM degradation potentials
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Christian Nyrop Albers*, Ole Stig Jacobsen, Jens Aamand
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Dept. Geochemistry, Geological Survey of Denmark & Greenland, Ø. Voldgade 10, DK-1350, Copenhagen,
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Denmark
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* Corresponding author. E-mail: cal@geus.dk, Tel.: +45 38142304
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Supplementary material
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Figure S1. The glass column setup for adhesion and degradation experiments. Eight filters could be run in parallel with
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downwards flow from either a common or individual influent. The multichannel pump ensured similar flows in all
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filters. The effluent could be sampled in bottles as shown or put to waste between sampling events.
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Figure S2. Breakthrough curves / tracer tests in glass column filters. a) 25 mL 100 mM NaCl solution in different filter
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materials. For simplicity, only results for quartz sand and Filtralite are shown, since additional filter materials were
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very similar to quartz sand. b) 25 mL inoculum eluted through a quartz sand filter with simultaneous measurement of
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conductivity and concentration of 14C-labeled cells.
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Figure S3. Pictures of filter materials used in the various experiments. Each picture is ~8 mm from left to right. Top left
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to right: Quartz sand 0.8-1.4 mm, Kerteminde post-filter, Hvidovre single filter. Middle left to right: Fe-coated quartz,
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Nevtraco, Filtralite. Bottom left to right: Astrup pre-filter, Aike post-filter.
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Figure S4. % adherence of the added MSH1 cells to filters containing quartz sand with varying cell density in the 25 mL
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inoculum of the glass column setup.
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a)
b)
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c)
d)
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e)
f)
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Figure S5. % adherence to filter material of the added MSH1 cells in the glass column and syringe setups as well as
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adherence to hexadecane in the MATH assay, a) with different filter materials. Astrup pre- and Aike post-filters were
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studied only with the syringe setup. b) at varying pH, c) at varying ionic strength values, d) as a function of time with
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no flow through the filter, e) after 7 days storage at 8°C or f) when already inoculated filters were reinoculated with
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either a standard inoculum or an inoculum obtained by combining and preconcentrating non-adhered cells from four
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filters (insert). Vertical bars are min-max of duplicate glass column filters, std. dev. of four replicates in the syringe
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setup and std. dev. of three replicates in the MATH assay.
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