1 Supplementary tables. 2 Article title: Shifting effects of rock roughness across a benthic food web 3 Journal: Hydrobiologia 4 Authors: Elizabeth A. Bergey and Joshua T. Cooper 5 Corresponding author: Elizabeth Bergey, University of Oklahoma; e-mail: lbergey@ou.edu 6 7 8 9 10 11 TABLE S1. Statistical summary of algal biomass as total and removed algal biomass in the constructed streambed rock clusters at the (a) start and (b) end of the colonization experiment. Rock clusters differed in rock type (shale, quartz-vein, sandstone), disturbance regime (undisturbed control, raked, scrubbed), and run location (upstream, downstream). Data were pooled across location (α = 0.05; N = 8). Non-significant interactions (Rock type x disturbance treatment) are not shown. 12 a) Initial algal biomass df F P ANOVA – stream location 1,70 0.000 0.999 MANOVA – total and removed algal biomass Rock type 4,124 22.43 <0.0001 Disturbance treatment 4,124 3.33 0.013 Rock type 2,63 59.76 <0.0001 Disturbance treatment 2,63 2.78 0.069 Rock type 2,63 7.62 0.0011 Disturbance treatment 2,63 6.78 0.0021 1,70 0.97 0.32 ANOVA: Total algal biomass ANOVA: Removed algal biomass b) Final algal biomass ANOVA – stream location MANOVA – total and removed algal biomass 1 Rock type 4,124 44.84 <0.0001 Disturbance treatment 4,124 2.43 0.051 ANOVA: Total algal biomass Rock type 2,63 119.19 <0.0001 Disturbance treatment 2,63 1.01 Rock type 2,63 17.071 <0.0001 Disturbance treatment 2,63 4.96 0.37 ANOVA: Removed algal biomass 13 14 2 0.010 15 TABLE S2. Statistical summary of the relationships in the distribution of grazing and predatory 16 macroinvertebrates among stream bed rock clusters that differed in rock type (shale, quartz-vein, 17 sandstone), disturbance regime (undisturbed control, raked, scrubbed), and run location (upstream, 18 downstream). Wilk’s Lambda values are shown MANOVA tests. α = 0.10; N = 4 for combined rock type, 19 treatment and location combinations). Non-significant interactions are not shown. Data are graphically 20 summarized in Fig. 3 df F P Rock type 4,106 2.09 0.088 Disturbance treatment 4,106 1.59 0.18 Location 2, 53 10.78 0.001 Rock type 2, 54 3.40 0.041 Disturbance treatment 2, 54 2.60 0.084 Location 1, 54 21.92 <0.0001 Rock type 2, 54 1.43 0.25 Disturbance treatment 2, 54 0.34 0.71 MANOVA– Grazers and predators ANOVA: Grazers ANOVA: Predators 3 Location 1, 54 2.87 0.096 Rock x disturbance x location 4, 54 2.40 0.061 21 22 23 24 4