Lab # _____: Exploring Biodiversity Where We Are Lab Skills: 3, 11, 12, 13, 15, 18, 23, 26, 28, 29 Background: “I am interested in the importance of plant diversity. For an ecologist, diversity means the number of different species in a community. A lot of research shows that communities with many species are healthier than those with just a few species. You may wonder what a healthy community looks like. Well, diverse plant communities usually produce bigger plants. Bigger plants mean more food and shelter for insects and other animals that live in those communities. Diverse plant communities are also more stable. Even if one species is having a bad year, there will be other species around to keep growing and supporting the ecosystem. Diverse plant communities also get fewer diseases and insect pests. Diverse communities are better for humans too!” Crossing Boundaries Conservation Scientist -- Susan Cook-Patton We are going to investigate plant and insect (arthropod) biodiversity right here on school grounds. Our area of focus has two habitat types: grass/turf and natural fields. You will hypothesize about the relative diversity of plants, flowers, and insects between these two habitat types and then conduct a brief field study to test your hypothesis. Working with your lab partner, you will collect data on plant and insect diversity in grass/turf and natural field habitats. At each site, you will need to keep track of different types of plants and insects by sampling some factors that influence biodiversity at each survey site. Hypothesis: We think that… (share which habitat you think will have more biodiversity.) Procedure: 1. Choose a study area for both grass/turf (A) and natural field (B) habitat types. Indicate your locations with an A or B on the map of the school property on your answer sheet and also provide a Figure 1. Google Earth view of Pulaski Middle – Senior High School property brief description of each location. 2. On your answer sheet, briefly describe the major vegetation types, density (how close together or far apart organisms are situated), and any other distinguishing characteristics of Location A – Grass/Turf Area and Location B – Natural field. Also report today’s weather and any unique or special circumstances that may influence your results. 1 3. Be sure to conduct your data collection in the following order at each location. This will ensure you don’t disturb the insects in your study area before you get a chance to count them. You just need to group insects by family. For plants, you don’t have to identify species but just keep track of each distinct variety that you find. A. Insect richness B. Plant richness C. Flower number D. Plant height 4. To begin, place the quadrat frame on the ground somewhere in the grass/turf location. Record the time of day that you sampled each location on your answer sheet. Remember, it is important that you count the insects within the entire quadrat frame first! Obtain the latitude and longitude data for your sample site from your teacher whom will be tracking each spot with a GPS device. 5. Now collect data and record onto your data collection answer sheet. As soon as you perform all counts and observe the biodiversity at the grass/turf location, continue on to the natural field area and repeat the procedure. 6. The last step is to pool all lab groups’ results and calculate overall averages for each of the four aspects of biodiversity that we investigated. These final results will allow us to form conclusions based on a large sample size. Analysis: A. How did your group’s results compare to the final class averages for insect richness and the number of plant species? B. Which habitat do you conclude has greater biodiversity? How does your conclusion relate to your original hypothesis for this investigation? C. Do you think today’s weather had a significant impact on your results? Why or why not? D. Were there any unique conditions or circumstances that may have influenced your results? If so, name them and explain what impact you think they had. E. Why are conclusions based on the class averages more reliable than they would be if they were based solely on your group’s data? I’m a stinkbug! I’m an aphid! I’m a praying mantis! I’m a weevil! 2 Name(s): ________________________________________________________________________________________ Exploring Biodiversity Where We Are Data Collection Answer Sheet Label location of A and B on map Brief description of major vegetation and other features Location A (Grass/Turf) Location B (Natural Field) Latitude and longitude Time of day Today’s weather Unique conditions that may influence results A. Insect Richness Tally – count the number of each type of insect and tally in the appropriate box. Type Location A Location B Ants Aphids Beetles Bees Butterflies / moths Caterpillars Crickets / grasshoppers Flies Ladybugs Larva / grubs Praying mantids Spiders Stinkbugs Weevils Other insects Total insects 3 B. Plant Species Checklist – count the number of plants of each type that are touching the string on your quadrat frame. You do not need to identify species, but you do need to keep track of which plant type is which letter. Type Location A Location B A B C D E F Total plant species C. Flower Tally – count each flower within your quadrat frame. Simply record the total number of flowers. Location A Location B # of flowers D. Plant Height – perform a measurement of plant height to the plant that touches the string crossing your quadrat frame every 20 cm. Record all measurements in cm rounded to the nearest tenth. Location A Location B Plant height (cm) Average plant height Class Results – pool results from all lab groups and calculate total class averages for Locations A and B. Location A Location B Insect Richness Tally Plant Species Checklist Flower Tally Plant Height 4